Identification and quantitative determination of a carboxylic and a mercapturic acid metabolite of etridiazole in urine of rat and man. Potential tools for biological monitoring

R.T.H. van Welie, R Mensert, P van Duyn, N P Vermeulen

Research output: Contribution to JournalArticleAcademicpeer-review

Abstract

Etridiazole, 5-ethoxy-3-trichloromethyl-1,2,4-thiadiazole, was orally administered to rats and human volunteers. Two metabolites of etridiazole were synthesized: 5-ethoxy-1,2,4-thiadiazole-3-carboxylic acid (ET-CA) and N-acetyl-S-(5-ethoxy-1,2,4-thiadiazol-3-yl-methyl)-L-cysteine (ET-MA). Selective and sensitive analytical procedures to determine etridiazole, the carboxylic acid ET-CA and the mercapturic acid ET-MA in urine were developed. The detection limit of etridiazole, applying GC with nitrogen selective detection (GC-NPD), was 36 micrograms/l urine (CV = 15.4%, n = 3). The detection limit of ET-CA, applying GC with sulphur selective detection (GC-FPD), was 100 micrograms/l urine (CV = 9.8%, n = 3). In urine of rats orally treated with etridiazole, ET-CA and ET-MA were identified as metabolites of etridiazole, whereas in urine of humans given oral etridiazole, only ET-CA was identified. Unmetabolized etridiazole was excreted for less than 0.1% of the administered dose in rats. ET-CA, however, accounted for 22 +/- 9% of the administered dose of etridiazole in rats and for 13 +/- 6% in humans. ET-MA appeared to be a minor urinary metabolite of etridiazole. ET-CA is proposed as a possible biomarker for the biological monitoring of etridiazole.

Original languageEnglish
Pages (from-to)625-32
Number of pages8
JournalArchives of Toxicology
Volume65
Issue number8
DOIs
Publication statusPublished - 1991

Keywords

  • Acetylcysteine
  • Adult
  • Animals
  • Carboxylic Acids
  • Environmental Monitoring
  • Fungicides, Industrial
  • Humans
  • Male
  • Rats
  • Rats, Inbred Strains
  • Thiadiazoles
  • Journal Article
  • Research Support, Non-U.S. Gov't

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