Identification of candidate transcriptional modulators involved in successful regeneration after nerve injury

F.J. Stam, H.D. MacGillavry, N.J. Armstrong, M.C.M. de Gunst, Y. Zhang, R.E. van Kesteren, A.B. Smit, J. Verhaagen

Research output: Contribution to JournalArticleAcademicpeer-review

Abstract

Successful regeneration of injured neurons requires a complex molecular response that involves the expression, modification and transport of a large number of proteins. The identity of neuronal proteins responsible for the initiation of regenerative neurite outgrowth is largely unknown. Dorsal root ganglion (DRG) neurons display robust and successful regeneration following lesion of their peripheral neurite, whereas outgrowth of central neurites is weak and does not lead to functional recovery. We have utilized this differential response to gain insight in the early transcriptional events associated with successful regeneration. Surprisingly, our study shows that peripheral and central nerve crushes elicit very distinct transcriptional activation, revealing a large set of novel genes that are differentially regulated within the first 24 h after the lesion. Here we show that Ankrd1, a gene known to act as a transcriptional modulator, is involved in neurite outgrowth of a DRG neuron-derived cell line as well as in cultured adult DRG neurons. This gene, and others identified in this study, may be part of the transcriptional regulatory module that orchestrates the onset of successful regeneration. © The Authors (2007).
Original languageEnglish
Pages (from-to)3629-3637
JournalEuropean Journal of Neuroscience
Volume25
Issue number12
DOIs
Publication statusPublished - 2007

Fingerprint

Nerve Regeneration
Regeneration
Spinal Ganglia
Neurons
Wounds and Injuries
Nerve Crush
Genes
Neurites
Peripheral Nerves
Transcriptional Activation
Proteins
Cell Line
Neuronal Outgrowth

Cite this

@article{a1704dafb49948c08d5c41231c1bfaa7,
title = "Identification of candidate transcriptional modulators involved in successful regeneration after nerve injury",
abstract = "Successful regeneration of injured neurons requires a complex molecular response that involves the expression, modification and transport of a large number of proteins. The identity of neuronal proteins responsible for the initiation of regenerative neurite outgrowth is largely unknown. Dorsal root ganglion (DRG) neurons display robust and successful regeneration following lesion of their peripheral neurite, whereas outgrowth of central neurites is weak and does not lead to functional recovery. We have utilized this differential response to gain insight in the early transcriptional events associated with successful regeneration. Surprisingly, our study shows that peripheral and central nerve crushes elicit very distinct transcriptional activation, revealing a large set of novel genes that are differentially regulated within the first 24 h after the lesion. Here we show that Ankrd1, a gene known to act as a transcriptional modulator, is involved in neurite outgrowth of a DRG neuron-derived cell line as well as in cultured adult DRG neurons. This gene, and others identified in this study, may be part of the transcriptional regulatory module that orchestrates the onset of successful regeneration. {\circledC} The Authors (2007).",
author = "F.J. Stam and H.D. MacGillavry and N.J. Armstrong and {de Gunst}, M.C.M. and Y. Zhang and {van Kesteren}, R.E. and A.B. Smit and J. Verhaagen",
year = "2007",
doi = "10.1111/j.1460-9568.2007.05597.x",
language = "English",
volume = "25",
pages = "3629--3637",
journal = "European Journal of Neuroscience",
issn = "0953-816X",
publisher = "Wiley-Blackwell",
number = "12",

}

Identification of candidate transcriptional modulators involved in successful regeneration after nerve injury. / Stam, F.J.; MacGillavry, H.D.; Armstrong, N.J.; de Gunst, M.C.M.; Zhang, Y.; van Kesteren, R.E.; Smit, A.B.; Verhaagen, J.

In: European Journal of Neuroscience, Vol. 25, No. 12, 2007, p. 3629-3637.

Research output: Contribution to JournalArticleAcademicpeer-review

TY - JOUR

T1 - Identification of candidate transcriptional modulators involved in successful regeneration after nerve injury

AU - Stam, F.J.

AU - MacGillavry, H.D.

AU - Armstrong, N.J.

AU - de Gunst, M.C.M.

AU - Zhang, Y.

AU - van Kesteren, R.E.

AU - Smit, A.B.

AU - Verhaagen, J.

PY - 2007

Y1 - 2007

N2 - Successful regeneration of injured neurons requires a complex molecular response that involves the expression, modification and transport of a large number of proteins. The identity of neuronal proteins responsible for the initiation of regenerative neurite outgrowth is largely unknown. Dorsal root ganglion (DRG) neurons display robust and successful regeneration following lesion of their peripheral neurite, whereas outgrowth of central neurites is weak and does not lead to functional recovery. We have utilized this differential response to gain insight in the early transcriptional events associated with successful regeneration. Surprisingly, our study shows that peripheral and central nerve crushes elicit very distinct transcriptional activation, revealing a large set of novel genes that are differentially regulated within the first 24 h after the lesion. Here we show that Ankrd1, a gene known to act as a transcriptional modulator, is involved in neurite outgrowth of a DRG neuron-derived cell line as well as in cultured adult DRG neurons. This gene, and others identified in this study, may be part of the transcriptional regulatory module that orchestrates the onset of successful regeneration. © The Authors (2007).

AB - Successful regeneration of injured neurons requires a complex molecular response that involves the expression, modification and transport of a large number of proteins. The identity of neuronal proteins responsible for the initiation of regenerative neurite outgrowth is largely unknown. Dorsal root ganglion (DRG) neurons display robust and successful regeneration following lesion of their peripheral neurite, whereas outgrowth of central neurites is weak and does not lead to functional recovery. We have utilized this differential response to gain insight in the early transcriptional events associated with successful regeneration. Surprisingly, our study shows that peripheral and central nerve crushes elicit very distinct transcriptional activation, revealing a large set of novel genes that are differentially regulated within the first 24 h after the lesion. Here we show that Ankrd1, a gene known to act as a transcriptional modulator, is involved in neurite outgrowth of a DRG neuron-derived cell line as well as in cultured adult DRG neurons. This gene, and others identified in this study, may be part of the transcriptional regulatory module that orchestrates the onset of successful regeneration. © The Authors (2007).

U2 - 10.1111/j.1460-9568.2007.05597.x

DO - 10.1111/j.1460-9568.2007.05597.x

M3 - Article

VL - 25

SP - 3629

EP - 3637

JO - European Journal of Neuroscience

JF - European Journal of Neuroscience

SN - 0953-816X

IS - 12

ER -