Identification of lactobacillus plantarum genes modulating, the cytokine response of human peripheral blood mononuclear cells.

S. van Hemert, M. Meijerink, D. Molenaar, P.A. Bron, D.L. de Vos, M. Kleerebezem, J.M. Wells, M.L. Marco

Research output: Contribution to JournalArticleAcademicpeer-review

Abstract

Background. Modulation of the immune system is one of the most plausible mechanisms underlying the beneficial effects of probiotic bacteria on human health. Presently, the specific probiotic cell products responsible for immunomodulation are largely unknown. In this study, the genetic and phenotypic diversity of strains of the Lactobacillus plantarum species were investigated to identify genes of L. plantarum with the potential to influence the amounts of cytokines interleukin 10 (IL-10) and IL-12 and the ratio of IL-10/IL-12 produced by peripheral blood mononuclear cells (PBMCs). Results. A total of 42 Lactobacillus plantarum strains isolated from diverse environmental and human sources were evaluated for their capacity to stimulate cytokine production in PBMCs. The L. plantarum strains induced the secretion of the anti-inflammatory cytokine IL-10 over an average 14-fold range and secretion of the pro-inflammatory cytokine IL-12 over an average 16-fold range. Comparisons of the strain-specific cytokine responses of PBMCs to comparative genome hybridization profiles obtained with L. plantarum WCFS1 DNA microarrays (also termed gene-trait matching) resulted in the identification of 6 candidate genetic loci with immunomodulatory capacities. These loci included genes encoding an N-acetyl-glucosamine/galactosamine phosphotransferase system, the LamBDCA quorum sensing system, and components of the plantaricin (bacteriocin) biosynthesis and transport pathway. Deletion of these genes in L. plantarum WCFS1 resulted in growth phase-dependent changes in the PBMC IL-10 and IL-12 cytokine profiles compared with wild-type cells. Conclusions. The altered PBMC cytokine profiles obtained with the L. plantarum WCFS1 mutants were in good agreement with the predictions made by gene-trait matching for the 42 L. plantarum strains. This study therefore resulted in the identification of genes present in certain strains of L. plantarum which might be responsible for the stimulation of anti- or pro-inflammatory immune responses in the gut. © 2010 van Hemert et al; licensee BioMed Central Ltd.
Original languageEnglish
Pages (from-to)293
JournalBMC Microbiology
Volume10
DOIs
Publication statusPublished - 2011

Fingerprint

Lactobacillus plantarum
Blood Cells
Cytokines
Interleukin-12
Genes
Interleukin-10
Probiotics
Anti-Inflammatory Agents
Quorum Sensing
Galactosamine
Bacteriocins
Genetic Loci
Comparative Genomic Hybridization
Immunomodulation
Glucosamine
Gene Deletion
Oligonucleotide Array Sequence Analysis
Immune System
Phosphotransferases
Bacteria

Cite this

van Hemert, S. ; Meijerink, M. ; Molenaar, D. ; Bron, P.A. ; de Vos, D.L. ; Kleerebezem, M. ; Wells, J.M. ; Marco, M.L. / Identification of lactobacillus plantarum genes modulating, the cytokine response of human peripheral blood mononuclear cells. In: BMC Microbiology. 2011 ; Vol. 10. pp. 293.
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title = "Identification of lactobacillus plantarum genes modulating, the cytokine response of human peripheral blood mononuclear cells.",
abstract = "Background. Modulation of the immune system is one of the most plausible mechanisms underlying the beneficial effects of probiotic bacteria on human health. Presently, the specific probiotic cell products responsible for immunomodulation are largely unknown. In this study, the genetic and phenotypic diversity of strains of the Lactobacillus plantarum species were investigated to identify genes of L. plantarum with the potential to influence the amounts of cytokines interleukin 10 (IL-10) and IL-12 and the ratio of IL-10/IL-12 produced by peripheral blood mononuclear cells (PBMCs). Results. A total of 42 Lactobacillus plantarum strains isolated from diverse environmental and human sources were evaluated for their capacity to stimulate cytokine production in PBMCs. The L. plantarum strains induced the secretion of the anti-inflammatory cytokine IL-10 over an average 14-fold range and secretion of the pro-inflammatory cytokine IL-12 over an average 16-fold range. Comparisons of the strain-specific cytokine responses of PBMCs to comparative genome hybridization profiles obtained with L. plantarum WCFS1 DNA microarrays (also termed gene-trait matching) resulted in the identification of 6 candidate genetic loci with immunomodulatory capacities. These loci included genes encoding an N-acetyl-glucosamine/galactosamine phosphotransferase system, the LamBDCA quorum sensing system, and components of the plantaricin (bacteriocin) biosynthesis and transport pathway. Deletion of these genes in L. plantarum WCFS1 resulted in growth phase-dependent changes in the PBMC IL-10 and IL-12 cytokine profiles compared with wild-type cells. Conclusions. The altered PBMC cytokine profiles obtained with the L. plantarum WCFS1 mutants were in good agreement with the predictions made by gene-trait matching for the 42 L. plantarum strains. This study therefore resulted in the identification of genes present in certain strains of L. plantarum which might be responsible for the stimulation of anti- or pro-inflammatory immune responses in the gut. {\circledC} 2010 van Hemert et al; licensee BioMed Central Ltd.",
author = "{van Hemert}, S. and M. Meijerink and D. Molenaar and P.A. Bron and {de Vos}, D.L. and M. Kleerebezem and J.M. Wells and M.L. Marco",
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Identification of lactobacillus plantarum genes modulating, the cytokine response of human peripheral blood mononuclear cells. / van Hemert, S.; Meijerink, M.; Molenaar, D.; Bron, P.A.; de Vos, D.L.; Kleerebezem, M.; Wells, J.M.; Marco, M.L.

In: BMC Microbiology, Vol. 10, 2011, p. 293.

Research output: Contribution to JournalArticleAcademicpeer-review

TY - JOUR

T1 - Identification of lactobacillus plantarum genes modulating, the cytokine response of human peripheral blood mononuclear cells.

AU - van Hemert, S.

AU - Meijerink, M.

AU - Molenaar, D.

AU - Bron, P.A.

AU - de Vos, D.L.

AU - Kleerebezem, M.

AU - Wells, J.M.

AU - Marco, M.L.

PY - 2011

Y1 - 2011

N2 - Background. Modulation of the immune system is one of the most plausible mechanisms underlying the beneficial effects of probiotic bacteria on human health. Presently, the specific probiotic cell products responsible for immunomodulation are largely unknown. In this study, the genetic and phenotypic diversity of strains of the Lactobacillus plantarum species were investigated to identify genes of L. plantarum with the potential to influence the amounts of cytokines interleukin 10 (IL-10) and IL-12 and the ratio of IL-10/IL-12 produced by peripheral blood mononuclear cells (PBMCs). Results. A total of 42 Lactobacillus plantarum strains isolated from diverse environmental and human sources were evaluated for their capacity to stimulate cytokine production in PBMCs. The L. plantarum strains induced the secretion of the anti-inflammatory cytokine IL-10 over an average 14-fold range and secretion of the pro-inflammatory cytokine IL-12 over an average 16-fold range. Comparisons of the strain-specific cytokine responses of PBMCs to comparative genome hybridization profiles obtained with L. plantarum WCFS1 DNA microarrays (also termed gene-trait matching) resulted in the identification of 6 candidate genetic loci with immunomodulatory capacities. These loci included genes encoding an N-acetyl-glucosamine/galactosamine phosphotransferase system, the LamBDCA quorum sensing system, and components of the plantaricin (bacteriocin) biosynthesis and transport pathway. Deletion of these genes in L. plantarum WCFS1 resulted in growth phase-dependent changes in the PBMC IL-10 and IL-12 cytokine profiles compared with wild-type cells. Conclusions. The altered PBMC cytokine profiles obtained with the L. plantarum WCFS1 mutants were in good agreement with the predictions made by gene-trait matching for the 42 L. plantarum strains. This study therefore resulted in the identification of genes present in certain strains of L. plantarum which might be responsible for the stimulation of anti- or pro-inflammatory immune responses in the gut. © 2010 van Hemert et al; licensee BioMed Central Ltd.

AB - Background. Modulation of the immune system is one of the most plausible mechanisms underlying the beneficial effects of probiotic bacteria on human health. Presently, the specific probiotic cell products responsible for immunomodulation are largely unknown. In this study, the genetic and phenotypic diversity of strains of the Lactobacillus plantarum species were investigated to identify genes of L. plantarum with the potential to influence the amounts of cytokines interleukin 10 (IL-10) and IL-12 and the ratio of IL-10/IL-12 produced by peripheral blood mononuclear cells (PBMCs). Results. A total of 42 Lactobacillus plantarum strains isolated from diverse environmental and human sources were evaluated for their capacity to stimulate cytokine production in PBMCs. The L. plantarum strains induced the secretion of the anti-inflammatory cytokine IL-10 over an average 14-fold range and secretion of the pro-inflammatory cytokine IL-12 over an average 16-fold range. Comparisons of the strain-specific cytokine responses of PBMCs to comparative genome hybridization profiles obtained with L. plantarum WCFS1 DNA microarrays (also termed gene-trait matching) resulted in the identification of 6 candidate genetic loci with immunomodulatory capacities. These loci included genes encoding an N-acetyl-glucosamine/galactosamine phosphotransferase system, the LamBDCA quorum sensing system, and components of the plantaricin (bacteriocin) biosynthesis and transport pathway. Deletion of these genes in L. plantarum WCFS1 resulted in growth phase-dependent changes in the PBMC IL-10 and IL-12 cytokine profiles compared with wild-type cells. Conclusions. The altered PBMC cytokine profiles obtained with the L. plantarum WCFS1 mutants were in good agreement with the predictions made by gene-trait matching for the 42 L. plantarum strains. This study therefore resulted in the identification of genes present in certain strains of L. plantarum which might be responsible for the stimulation of anti- or pro-inflammatory immune responses in the gut. © 2010 van Hemert et al; licensee BioMed Central Ltd.

U2 - 10.1186/1471-2180-10-293

DO - 10.1186/1471-2180-10-293

M3 - Article

VL - 10

SP - 293

JO - BMC Microbiology

JF - BMC Microbiology

SN - 1471-2180

ER -