Identification of novel PANDAR protein interaction partners involved in splicing regulation

N. Pospiech, H. Cibis, L. Dietrich, F. Müller, T. Bange, S. Hennig

Research output: Contribution to JournalArticleAcademicpeer-review

Abstract

Interactions of long non-coding RNAs (lncRNA) with proteins play important roles in the regulation of many cellular processes. PANDAR (Promotor of CDKN1A Antisense DNA damage Activated RNA) is a lncRNA that is transcribed in a p53-dependent manner from the CDKN1A promoter and is involved in the regulation of proliferation and senescence. Overexpression of PANDAR has been observed in several tumor species and correlated with a poor prognosis for patient survival rate. Depending on the cellular state, PANDAR is known to interact with proteins such as the nuclear transcription factor Y subunit A (NF-YA) and the scaffold attachment factor A (SAF-A). However, a comprehensive analysis of the PANDAR interactome was missing so far. Therefore, we applied peptide nucleic acid (PNA)-based pull-downs combined with quantitative mass spectrometry to identify new protein binding partners. We confirmed potential candidates like U2AF65 and PTBP1, known to be involved in RNA processing. Furthermore, we observed that overexpression of PANDAR leads to a reduced level of the short pro-apoptotic BCL-X splice variant (BCL-XS) which is regulated by PTBP1. Simultaneous overexpression of PTBP1 was able to rescue this effect. Overall, our data suggest a role for PANDAR in the regulation of splicing events via its interaction partner PTBP1.

Original languageEnglish
Article number2798
Pages (from-to)1-9
Number of pages9
JournalScientific Reports
Volume8
Issue number1
DOIs
Publication statusPublished - 12 Feb 2018

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Antisense DNA
DNA Damage
RNA
Long Noncoding RNA
Proteins
Heterogeneous-Nuclear Ribonucleoprotein U
Peptide Nucleic Acids
Protein Binding
Mass Spectrometry
Transcription Factors
Survival Rate

Cite this

Pospiech, N. ; Cibis, H. ; Dietrich, L. ; Müller, F. ; Bange, T. ; Hennig, S. / Identification of novel PANDAR protein interaction partners involved in splicing regulation. In: Scientific Reports. 2018 ; Vol. 8, No. 1. pp. 1-9.
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Identification of novel PANDAR protein interaction partners involved in splicing regulation. / Pospiech, N.; Cibis, H.; Dietrich, L.; Müller, F.; Bange, T.; Hennig, S.

In: Scientific Reports, Vol. 8, No. 1, 2798, 12.02.2018, p. 1-9.

Research output: Contribution to JournalArticleAcademicpeer-review

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