Imatinib mesylate does not counteract ovarian tissue fibrosis in postnatal rat ovary

Babak Asadi-Azarbaijani, Saskia Braber, Majorie van Duursen, Kirsi Jahnukainen, Regiane Santos, Irma Oskam

Research output: Contribution to JournalArticleAcademicpeer-review

Abstract

Chemotherapy may result in ovarian atrophy, a depletion of the primordial follicle pool, diminished ovarian weight, cortical and stromal fibrosis. Imatinib mesylate is an anticancer agent that inhibits competitively several receptor tyrosine kinases (RTKs). RTKs play important roles in cell metabolism, proliferation, and apoptosis. In clinic, imatinib mesylate is also known as an anti-fibrotic medicine. In the present study, the impact of imatinib on the ovarian tissue was investigated by assessing ovarian tissue fibrosis in postnatal rat administered with or without imatinib for three days. Fibrosis in the ovarian tissue was determined by histology (Picrosirius and Masson's trichrome staining) and the protein expression of vimentin and alpha-smooth muscle actin (α-SMA). Furthermore, mRNA expression of Forkhead box transcription factor O1 and O3 (FOXO1 and FOXO3), which are markers of cell proliferation was quantified. A short-term exposure to imatinib showed to increase tissue fibrosis in ovaries. This was observed by Masson's trichrome staining. Exposure to imatinib led also to a down-regulation of vimentin protein expression and up-regulation mRNA expression of FOXO3. This may indicate a role of FOXO3 in ovarian tissue fibrosis in postnatal rat ovaries.

Original languageEnglish
Pages (from-to)133-138
Number of pages6
JournalReproductive Biology
Volume19
Issue number2
Early online date10 May 2019
DOIs
Publication statusPublished - Jun 2019

Fingerprint

fibrosis
Ovary
Fibrosis
rats
vimentin
Receptor Protein-Tyrosine Kinases
Vimentin
tyrosine
phosphotransferases (kinases)
protein synthesis
receptors
Cell Proliferation
Staining and Labeling
antineoplastic agents
Forkhead Transcription Factors
Messenger RNA
atrophy
smooth muscle
histology
drug therapy

Keywords

  • FOXO1
  • FOXO3
  • Imatinib
  • Ovary
  • Tissue fibrosis

Cite this

Asadi-Azarbaijani, Babak ; Braber, Saskia ; van Duursen, Majorie ; Jahnukainen, Kirsi ; Santos, Regiane ; Oskam, Irma. / Imatinib mesylate does not counteract ovarian tissue fibrosis in postnatal rat ovary. In: Reproductive Biology. 2019 ; Vol. 19, No. 2. pp. 133-138.
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abstract = "Chemotherapy may result in ovarian atrophy, a depletion of the primordial follicle pool, diminished ovarian weight, cortical and stromal fibrosis. Imatinib mesylate is an anticancer agent that inhibits competitively several receptor tyrosine kinases (RTKs). RTKs play important roles in cell metabolism, proliferation, and apoptosis. In clinic, imatinib mesylate is also known as an anti-fibrotic medicine. In the present study, the impact of imatinib on the ovarian tissue was investigated by assessing ovarian tissue fibrosis in postnatal rat administered with or without imatinib for three days. Fibrosis in the ovarian tissue was determined by histology (Picrosirius and Masson's trichrome staining) and the protein expression of vimentin and alpha-smooth muscle actin (α-SMA). Furthermore, mRNA expression of Forkhead box transcription factor O1 and O3 (FOXO1 and FOXO3), which are markers of cell proliferation was quantified. A short-term exposure to imatinib showed to increase tissue fibrosis in ovaries. This was observed by Masson's trichrome staining. Exposure to imatinib led also to a down-regulation of vimentin protein expression and up-regulation mRNA expression of FOXO3. This may indicate a role of FOXO3 in ovarian tissue fibrosis in postnatal rat ovaries.",
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Asadi-Azarbaijani, B, Braber, S, van Duursen, M, Jahnukainen, K, Santos, R & Oskam, I 2019, 'Imatinib mesylate does not counteract ovarian tissue fibrosis in postnatal rat ovary' Reproductive Biology, vol. 19, no. 2, pp. 133-138. https://doi.org/10.1016/j.repbio.2019.03.003

Imatinib mesylate does not counteract ovarian tissue fibrosis in postnatal rat ovary. / Asadi-Azarbaijani, Babak; Braber, Saskia; van Duursen, Majorie; Jahnukainen, Kirsi; Santos, Regiane; Oskam, Irma.

In: Reproductive Biology, Vol. 19, No. 2, 06.2019, p. 133-138.

Research output: Contribution to JournalArticleAcademicpeer-review

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