Immunocytochemical detection of prostaglandin E2 in microvasculature and in neurons of rat brain after administration of bacterial endotoxin

A M Van Dam; M Brouns; W Man-A-Hing; F Berkenbosch

doi:10.1016/0006-8993(93)90922-A

Immunocytochemical detection of prostaglandin E2 in microvasculature and in neurons of rat brain after administration of bacterial endotoxin

A M Van Dam, M Brouns, W Man-A-Hing, F Berkenbosch

Research output: Contribution to Journal › Article › Academic › peer-review

Abstract

The aim of the present study was to identify the site of prostaglandin E2 (PGE2) production in the brain in response to a pyrogenic dose of endotoxin. The presence of PGE2 was detected using immunocytochemistry on Bouin's fixed vibratome sections of control and endotoxin-treated rats. Peripheral administration of endotoxin caused a time-related stimulation of PGE2 immunoreactivity (irPGE2) in the choroid plexus and in the microvasculature of the brain. In addition to these sites, hypophysiotrophic neurons of the paraventricular nucleus (PVN) and supraopticus nucleus (SON) responded with induction of irPGE2 to endotoxin administration. Our data demonstrate that alterations in brain function in response to endotoxin may involve arachidonic metabolites such as PGE2 that are induced at the blood-brain barrier (microvasculature) and blood-liquor barrier (choroid plexus) and in hypophysiotrophic neurons of the rat brain.

Original language	English
Pages (from-to)	331-6
Number of pages	6
Journal	Brain Research
Volume	613
Issue number	2
DOIs	https://doi.org/10.1016/0006-8993(93)90922-A https://doi.org/10.1016/0006-8993(93)90922-A
Publication status	Published - 11 Jun 1993

Keywords

Animals
Brain/drug effects
Cerebrovascular Circulation
Dinoprostone/analysis
Endotoxins/toxicity
Immunohistochemistry/methods
Male
Microcirculation/drug effects
Neurons/drug effects
Organ Specificity
Rats
Rats, Wistar
Reference Values

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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title = "Immunocytochemical detection of prostaglandin E2 in microvasculature and in neurons of rat brain after administration of bacterial endotoxin",

abstract = "The aim of the present study was to identify the site of prostaglandin E2 (PGE2) production in the brain in response to a pyrogenic dose of endotoxin. The presence of PGE2 was detected using immunocytochemistry on Bouin's fixed vibratome sections of control and endotoxin-treated rats. Peripheral administration of endotoxin caused a time-related stimulation of PGE2 immunoreactivity (irPGE2) in the choroid plexus and in the microvasculature of the brain. In addition to these sites, hypophysiotrophic neurons of the paraventricular nucleus (PVN) and supraopticus nucleus (SON) responded with induction of irPGE2 to endotoxin administration. Our data demonstrate that alterations in brain function in response to endotoxin may involve arachidonic metabolites such as PGE2 that are induced at the blood-brain barrier (microvasculature) and blood-liquor barrier (choroid plexus) and in hypophysiotrophic neurons of the rat brain.",

keywords = "Animals, Brain/drug effects, Cerebrovascular Circulation, Dinoprostone/analysis, Endotoxins/toxicity, Immunohistochemistry/methods, Male, Microcirculation/drug effects, Neurons/drug effects, Organ Specificity, Rats, Rats, Wistar, Reference Values",

author = "{Van Dam}, {A M} and M Brouns and W Man-A-Hing and F Berkenbosch",

year = "1993",

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doi = "10.1016/0006-8993(93)90922-A",

language = "English",

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T1 - Immunocytochemical detection of prostaglandin E2 in microvasculature and in neurons of rat brain after administration of bacterial endotoxin

AU - Van Dam, A M

AU - Brouns, M

AU - Man-A-Hing, W

AU - Berkenbosch, F

PY - 1993/6/11

Y1 - 1993/6/11

N2 - The aim of the present study was to identify the site of prostaglandin E2 (PGE2) production in the brain in response to a pyrogenic dose of endotoxin. The presence of PGE2 was detected using immunocytochemistry on Bouin's fixed vibratome sections of control and endotoxin-treated rats. Peripheral administration of endotoxin caused a time-related stimulation of PGE2 immunoreactivity (irPGE2) in the choroid plexus and in the microvasculature of the brain. In addition to these sites, hypophysiotrophic neurons of the paraventricular nucleus (PVN) and supraopticus nucleus (SON) responded with induction of irPGE2 to endotoxin administration. Our data demonstrate that alterations in brain function in response to endotoxin may involve arachidonic metabolites such as PGE2 that are induced at the blood-brain barrier (microvasculature) and blood-liquor barrier (choroid plexus) and in hypophysiotrophic neurons of the rat brain.

AB - The aim of the present study was to identify the site of prostaglandin E2 (PGE2) production in the brain in response to a pyrogenic dose of endotoxin. The presence of PGE2 was detected using immunocytochemistry on Bouin's fixed vibratome sections of control and endotoxin-treated rats. Peripheral administration of endotoxin caused a time-related stimulation of PGE2 immunoreactivity (irPGE2) in the choroid plexus and in the microvasculature of the brain. In addition to these sites, hypophysiotrophic neurons of the paraventricular nucleus (PVN) and supraopticus nucleus (SON) responded with induction of irPGE2 to endotoxin administration. Our data demonstrate that alterations in brain function in response to endotoxin may involve arachidonic metabolites such as PGE2 that are induced at the blood-brain barrier (microvasculature) and blood-liquor barrier (choroid plexus) and in hypophysiotrophic neurons of the rat brain.

KW - Animals

KW - Brain/drug effects

KW - Cerebrovascular Circulation

KW - Dinoprostone/analysis

KW - Endotoxins/toxicity

KW - Immunohistochemistry/methods

KW - Male

KW - Microcirculation/drug effects

KW - Neurons/drug effects

KW - Organ Specificity

KW - Rats

KW - Rats, Wistar

KW - Reference Values

U2 - 10.1016/0006-8993(93)90922-A

DO - 10.1016/0006-8993(93)90922-A

M3 - Article

C2 - 8186987

SN - 0006-8993

VL - 613

SP - 331

EP - 336

JO - Brain Research

JF - Brain Research

IS - 2

ER -

Immunocytochemical detection of prostaglandin E2 in microvasculature and in neurons of rat brain after administration of bacterial endotoxin

Abstract

Keywords

UN SDGs

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