In or out? On the tightness of glycosomal compartmentalizatin of metabolites and enzymes in Trypanosoma brucei.

J.R. Haanstra, B.M. Bakker, P.A.M. Michels

Research output: Contribution to JournalArticleAcademicpeer-review

Abstract

Trypanosomatids sequester large parts of glucose metabolism inside specialised peroxisomes, called glycosomes. Many studies have shown that correct glycosomal compartmentalization of glycolytic enzymes is essential for bloodstream-form Trypanosoma brucei. The recent finding of pore-forming activities in glycosomal membrane preparations and extensions of the trypanosome glycolysis computer model with size-selective pores sparked again an old debate on the extent of (im)permeability of the glycosomal membrane and whether glycosomally located glycolytic enzymes could and should also be present with some activity in the cytosol. This review presents a critical discussion of the experimental and theoretical evidence for and against the different hypotheses.
Original languageEnglish
Pages (from-to)18-28
JournalMolecular and Biochemical Parasitology
Volume198
DOIs
Publication statusPublished - 2014

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Trypanosoma brucei brucei
Microbodies
Peroxisomes
Membranes
Trypanosomiasis
Glycolysis
Enzymes
Computer Simulation
Cytosol
Permeability
Glucose

Cite this

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title = "In or out? On the tightness of glycosomal compartmentalizatin of metabolites and enzymes in Trypanosoma brucei.",
abstract = "Trypanosomatids sequester large parts of glucose metabolism inside specialised peroxisomes, called glycosomes. Many studies have shown that correct glycosomal compartmentalization of glycolytic enzymes is essential for bloodstream-form Trypanosoma brucei. The recent finding of pore-forming activities in glycosomal membrane preparations and extensions of the trypanosome glycolysis computer model with size-selective pores sparked again an old debate on the extent of (im)permeability of the glycosomal membrane and whether glycosomally located glycolytic enzymes could and should also be present with some activity in the cytosol. This review presents a critical discussion of the experimental and theoretical evidence for and against the different hypotheses.",
author = "J.R. Haanstra and B.M. Bakker and P.A.M. Michels",
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In or out? On the tightness of glycosomal compartmentalizatin of metabolites and enzymes in Trypanosoma brucei. / Haanstra, J.R.; Bakker, B.M.; Michels, P.A.M.

In: Molecular and Biochemical Parasitology, Vol. 198, 2014, p. 18-28.

Research output: Contribution to JournalArticleAcademicpeer-review

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AU - Bakker, B.M.

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PY - 2014

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U2 - 10.1016/j.molbiopara.2014.11.004

DO - 10.1016/j.molbiopara.2014.11.004

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