TY - JOUR
T1 - In vitro inhibition of rat and human glutathione S-transferase isoenzymes by disulfiram and diethyldithiocarbamate.
AU - Ploemen, J.P.H.T.M.
AU - van Iersel, M.
AU - Wormhoudt, L.W.
AU - Commandeur, J.N.M.
AU - Vermeulen, N.P.E.
AU - van Bladeren, P.J.
PY - 1996
Y1 - 1996
N2 - The drug disulfiram (DSF, Antabuse®) has been used in the therapy of alcohol abuse. It is a potent inhibitor of aldehyde dehydrogenase. Its reduced form, diethyldithiocarbamate (DDTC), and further metabolites show similar activities. DSF and DDTC have also been widely used to inhibit mixed-function oxidases. In this study, the reversible inhibition and time-dependent inactivation of the major rat and human glutathione S-transferase (GST) isoenzymes by DSF and DDTC was investigated. Reversible inhibition, using 1-chloro-2,4-dinitrobenzene as substrate for the GST alpha-, mu-, and pi-class, expressed as I
AB - The drug disulfiram (DSF, Antabuse®) has been used in the therapy of alcohol abuse. It is a potent inhibitor of aldehyde dehydrogenase. Its reduced form, diethyldithiocarbamate (DDTC), and further metabolites show similar activities. DSF and DDTC have also been widely used to inhibit mixed-function oxidases. In this study, the reversible inhibition and time-dependent inactivation of the major rat and human glutathione S-transferase (GST) isoenzymes by DSF and DDTC was investigated. Reversible inhibition, using 1-chloro-2,4-dinitrobenzene as substrate for the GST alpha-, mu-, and pi-class, expressed as I
U2 - 10.1016/0006-2952(96)00142-6
DO - 10.1016/0006-2952(96)00142-6
M3 - Article
VL - 52
SP - 197
EP - 204
JO - Biochemical Pharmacology
JF - Biochemical Pharmacology
SN - 0006-2952
ER -
