Abstract
In vitro three-dimensional models for metastatic ovarian cancer have been useful for recapitulating the human disease. These spheroidal tumor cultures, however, can grow in excess of 1 mm in diameter, which are difficult to visualize without suitable imaging technology. Optical coherence tomography (OCT) is an ideal live imaging method for non-perturbatively visualizing these complex systems. OCT enabled detailed observations of the model at both nodular and cellular levels, revealing growth dynamics not previously observed. The development ofa time-lapse OCT system, capable of automated, multidimensional acquisition, further provided insights into the growth and chemotherapeutic response of ovarian cancer.© 2009 Optical Society of America.
Original language | English |
---|---|
Pages (from-to) | 8892-8906 |
Number of pages | 15 |
Journal | Optics Express |
Volume | 17 |
Issue number | 11 |
DOIs | |
Publication status | Published - 2009 |