In vitro recognition of an orf virus early promoter in a vaccinia virus extract

J C Vos, A. A. Mercer, S B Fleming, A J Robinson

Research output: Contribution to JournalArticleAcademicpeer-review

Abstract

DNA fragments containing varying lengths of the 5' end of an orf virus early gene (ORF3) and its associated promoter were introduced into sodium deoxycholate-solubilized vaccinia virus extracts capable of initiating transcription in vitro from vaccinia virus early promoters. After separation of the radiolabelled products of the reactions on a 5% polyacrylamide/7 M urea gel, discrete transcripts were detected the sizes of which were consistent with initiation of transcription from the orf virus early promoter. This is the first demonstration in a functional assay of the conservation of early transcriptional promoters between an orthopoxvirus and a parapoxvirus.

Original languageEnglish
Pages (from-to)223-8
Number of pages6
JournalArchives of Virology
Volume123
Issue number1-2
DOIs
Publication statusPublished - 1992

Keywords

  • Base Sequence
  • Cloning, Molecular
  • DNA Restriction Enzymes
  • DNA, Viral
  • DNA-Directed DNA Polymerase
  • Molecular Sequence Data
  • Orf virus
  • Promoter Regions, Genetic
  • Transcription, Genetic
  • Vaccinia virus
  • Journal Article

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