Abstract
DNA fragments containing varying lengths of the 5' end of an orf virus early gene (ORF3) and its associated promoter were introduced into sodium deoxycholate-solubilized vaccinia virus extracts capable of initiating transcription in vitro from vaccinia virus early promoters. After separation of the radiolabelled products of the reactions on a 5% polyacrylamide/7 M urea gel, discrete transcripts were detected the sizes of which were consistent with initiation of transcription from the orf virus early promoter. This is the first demonstration in a functional assay of the conservation of early transcriptional promoters between an orthopoxvirus and a parapoxvirus.
Original language | English |
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Pages (from-to) | 223-8 |
Number of pages | 6 |
Journal | Archives of Virology |
Volume | 123 |
Issue number | 1-2 |
DOIs | |
Publication status | Published - 1992 |
Keywords
- Base Sequence
- Cloning, Molecular
- DNA Restriction Enzymes
- DNA, Viral
- DNA-Directed DNA Polymerase
- Molecular Sequence Data
- Orf virus
- Promoter Regions, Genetic
- Transcription, Genetic
- Vaccinia virus
- Journal Article