In vitro recognition of an orf virus early promoter in a vaccinia virus extract

J C Vos; A. A. Mercer; S B Fleming; A J Robinson

doi:10.1007/BF01317152

In vitro recognition of an orf virus early promoter in a vaccinia virus extract

J C Vos, A. A. Mercer, S B Fleming, A J Robinson

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Research output: Contribution to Journal › Article › Academic › peer-review

Abstract

DNA fragments containing varying lengths of the 5' end of an orf virus early gene (ORF3) and its associated promoter were introduced into sodium deoxycholate-solubilized vaccinia virus extracts capable of initiating transcription in vitro from vaccinia virus early promoters. After separation of the radiolabelled products of the reactions on a 5% polyacrylamide/7 M urea gel, discrete transcripts were detected the sizes of which were consistent with initiation of transcription from the orf virus early promoter. This is the first demonstration in a functional assay of the conservation of early transcriptional promoters between an orthopoxvirus and a parapoxvirus.

Original language	English
Pages (from-to)	223-8
Number of pages	6
Journal	Archives of Virology
Volume	123
Issue number	1-2
DOIs	https://doi.org/10.1007/BF01317152
Publication status	Published - 1992

Keywords

Base Sequence
Cloning, Molecular
DNA Restriction Enzymes
DNA, Viral
DNA-Directed DNA Polymerase
Molecular Sequence Data
Orf virus
Promoter Regions, Genetic
Transcription, Genetic
Vaccinia virus
Journal Article

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This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1007/BF01317152

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title = "In vitro recognition of an orf virus early promoter in a vaccinia virus extract",

abstract = "DNA fragments containing varying lengths of the 5' end of an orf virus early gene (ORF3) and its associated promoter were introduced into sodium deoxycholate-solubilized vaccinia virus extracts capable of initiating transcription in vitro from vaccinia virus early promoters. After separation of the radiolabelled products of the reactions on a 5% polyacrylamide/7 M urea gel, discrete transcripts were detected the sizes of which were consistent with initiation of transcription from the orf virus early promoter. This is the first demonstration in a functional assay of the conservation of early transcriptional promoters between an orthopoxvirus and a parapoxvirus.",

keywords = "Base Sequence, Cloning, Molecular, DNA Restriction Enzymes, DNA, Viral, DNA-Directed DNA Polymerase, Molecular Sequence Data, Orf virus, Promoter Regions, Genetic, Transcription, Genetic, Vaccinia virus, Journal Article",

author = "Vos, {J C} and Mercer, {A. A.} and Fleming, {S B} and Robinson, {A J}",

year = "1992",

doi = "10.1007/BF01317152",

language = "English",

volume = "123",

pages = "223--8",

journal = "Archives of Virology",

issn = "0304-8608",

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T1 - In vitro recognition of an orf virus early promoter in a vaccinia virus extract

AU - Vos, J C

AU - Mercer, A. A.

AU - Fleming, S B

AU - Robinson, A J

PY - 1992

Y1 - 1992

N2 - DNA fragments containing varying lengths of the 5' end of an orf virus early gene (ORF3) and its associated promoter were introduced into sodium deoxycholate-solubilized vaccinia virus extracts capable of initiating transcription in vitro from vaccinia virus early promoters. After separation of the radiolabelled products of the reactions on a 5% polyacrylamide/7 M urea gel, discrete transcripts were detected the sizes of which were consistent with initiation of transcription from the orf virus early promoter. This is the first demonstration in a functional assay of the conservation of early transcriptional promoters between an orthopoxvirus and a parapoxvirus.

AB - DNA fragments containing varying lengths of the 5' end of an orf virus early gene (ORF3) and its associated promoter were introduced into sodium deoxycholate-solubilized vaccinia virus extracts capable of initiating transcription in vitro from vaccinia virus early promoters. After separation of the radiolabelled products of the reactions on a 5% polyacrylamide/7 M urea gel, discrete transcripts were detected the sizes of which were consistent with initiation of transcription from the orf virus early promoter. This is the first demonstration in a functional assay of the conservation of early transcriptional promoters between an orthopoxvirus and a parapoxvirus.

KW - Base Sequence

KW - Cloning, Molecular

KW - DNA Restriction Enzymes

KW - DNA, Viral

KW - DNA-Directed DNA Polymerase

KW - Molecular Sequence Data

KW - Orf virus

KW - Promoter Regions, Genetic

KW - Transcription, Genetic

KW - Vaccinia virus

KW - Journal Article

U2 - 10.1007/BF01317152

DO - 10.1007/BF01317152

M3 - Article

C2 - 1312824

SN - 0304-8608

VL - 123

SP - 223

EP - 228

JO - Archives of Virology

JF - Archives of Virology

IS - 1-2

ER -

In vitro recognition of an orf virus early promoter in a vaccinia virus extract

Abstract

Keywords

UN SDGs

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