Abstract
DNA fragments containing varying lengths of the 5' end of an orf virus early gene (ORF3) and its associated promoter were introduced into sodium deoxycholate-solubilized vaccinia virus extracts capable of initiating transcription in vitro from vaccinia virus early promoters. After separation of the radiolabelled products of the reactions on a 5% polyacrylamide/7 M urea gel, discrete transcripts were detected the sizes of which were consistent with initiation of transcription from the orf virus early promoter. This is the first demonstration in a functional assay of the conservation of early transcriptional promoters between an orthopoxvirus and a parapoxvirus.
| Original language | English |
|---|---|
| Pages (from-to) | 223-8 |
| Number of pages | 6 |
| Journal | Archives of Virology |
| Volume | 123 |
| Issue number | 1-2 |
| DOIs | |
| Publication status | Published - 1992 |
UN SDGs
This output contributes to the following UN Sustainable Development Goals (SDGs)
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SDG 3 Good Health and Well-being
Keywords
- Base Sequence
- Cloning, Molecular
- DNA Restriction Enzymes
- DNA, Viral
- DNA-Directed DNA Polymerase
- Molecular Sequence Data
- Orf virus
- Promoter Regions, Genetic
- Transcription, Genetic
- Vaccinia virus
- Journal Article
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