In vivo relevance of two critical levels for NAD(P)H:quinone oxidoreductase (NQO1)-mediated cellular protection against electrophile toxicity found in vitro.

L.H. de Haan; G.K. Pot; J.M. Aarts; I.M. Rietjens; G.M. Alink

doi:10.1016/j.tiv.2005.10.005

In vivo relevance of two critical levels for NAD(P)H:quinone oxidoreductase (NQO1)-mediated cellular protection against electrophile toxicity found in vitro.

L.H. de Haan, G.K. Pot, J.M. Aarts, I.M. Rietjens, G.M. Alink

Mathematics

Research output: Contribution to Journal › Article › Academic › peer-review

Abstract

NAD(P)H:quinone oxidoreductase (NQO1)-mediated detoxification of quinones is suggested to be involved in cancer prevention. In the present study, using transfected CHO cells, it was demonstrated that the relation between NQO1 activity and the resulting protection against the cytotoxicity of menadione shows a steep dose-response curve revealing a 'lower protection threshold' of 0.5 μmol DCPIP/min/mg protein and an 'upper protection threshold' at 1 μmol DCPIP/min/mg protein. In an additional in vivo experiment it was investigated how both in vitro critical activity levels of NQO1, relate to NQO1 activities in mice and man, either without or upon induction of the enzyme by butylated hydroxyanisol (BHA) or indole-3-carbinol (I

Original language	English
Pages (from-to)	594-600
Journal	Toxicology in Vitro
Volume	20
Issue number	5
DOIs	https://doi.org/10.1016/j.tiv.2005.10.005
Publication status	Published - 2006

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10.1016/j.tiv.2005.10.005

http://www.sciencedirect.com/science/article/pii/S0887233305002213

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title = "In vivo relevance of two critical levels for NAD(P)H:quinone oxidoreductase (NQO1)-mediated cellular protection against electrophile toxicity found in vitro.",

abstract = "NAD(P)H:quinone oxidoreductase (NQO1)-mediated detoxification of quinones is suggested to be involved in cancer prevention. In the present study, using transfected CHO cells, it was demonstrated that the relation between NQO1 activity and the resulting protection against the cytotoxicity of menadione shows a steep dose-response curve revealing a 'lower protection threshold' of 0.5 μmol DCPIP/min/mg protein and an 'upper protection threshold' at 1 μmol DCPIP/min/mg protein. In an additional in vivo experiment it was investigated how both in vitro critical activity levels of NQO1, relate to NQO1 activities in mice and man, either without or upon induction of the enzyme by butylated hydroxyanisol (BHA) or indole-3-carbinol (I",

author = "{de Haan}, L.H. and G.K. Pot and J.M. Aarts and I.M. Rietjens and G.M. Alink",

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T1 - In vivo relevance of two critical levels for NAD(P)H:quinone oxidoreductase (NQO1)-mediated cellular protection against electrophile toxicity found in vitro.

AU - de Haan, L.H.

AU - Pot, G.K.

AU - Aarts, J.M.

AU - Rietjens, I.M.

AU - Alink, G.M.

PY - 2006

Y1 - 2006

N2 - NAD(P)H:quinone oxidoreductase (NQO1)-mediated detoxification of quinones is suggested to be involved in cancer prevention. In the present study, using transfected CHO cells, it was demonstrated that the relation between NQO1 activity and the resulting protection against the cytotoxicity of menadione shows a steep dose-response curve revealing a 'lower protection threshold' of 0.5 μmol DCPIP/min/mg protein and an 'upper protection threshold' at 1 μmol DCPIP/min/mg protein. In an additional in vivo experiment it was investigated how both in vitro critical activity levels of NQO1, relate to NQO1 activities in mice and man, either without or upon induction of the enzyme by butylated hydroxyanisol (BHA) or indole-3-carbinol (I

AB - NAD(P)H:quinone oxidoreductase (NQO1)-mediated detoxification of quinones is suggested to be involved in cancer prevention. In the present study, using transfected CHO cells, it was demonstrated that the relation between NQO1 activity and the resulting protection against the cytotoxicity of menadione shows a steep dose-response curve revealing a 'lower protection threshold' of 0.5 μmol DCPIP/min/mg protein and an 'upper protection threshold' at 1 μmol DCPIP/min/mg protein. In an additional in vivo experiment it was investigated how both in vitro critical activity levels of NQO1, relate to NQO1 activities in mice and man, either without or upon induction of the enzyme by butylated hydroxyanisol (BHA) or indole-3-carbinol (I

U2 - 10.1016/j.tiv.2005.10.005

DO - 10.1016/j.tiv.2005.10.005

M3 - Article

SN - 0887-2333

VL - 20

SP - 594

EP - 600

JO - Toxicology in Vitro

JF - Toxicology in Vitro

IS - 5

ER -

In vivo relevance of two critical levels for NAD(P)H:quinone oxidoreductase (NQO1)-mediated cellular protection against electrophile toxicity found in vitro.

Abstract

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