In vivo relevance of two critical levels for NAD(P)H:quinone oxidoreductase (NQO1)-mediated cellular protection against electrophile toxicity found in vitro.

L.H. de Haan, G.K. Pot, J.M. Aarts, I.M. Rietjens, G.M. Alink

Research output: Contribution to JournalArticleAcademicpeer-review

Abstract

NAD(P)H:quinone oxidoreductase (NQO1)-mediated detoxification of quinones is suggested to be involved in cancer prevention. In the present study, using transfected CHO cells, it was demonstrated that the relation between NQO1 activity and the resulting protection against the cytotoxicity of menadione shows a steep dose-response curve revealing a 'lower protection threshold' of 0.5 μmol DCPIP/min/mg protein and an 'upper protection threshold' at 1 μmol DCPIP/min/mg protein. In an additional in vivo experiment it was investigated how both in vitro critical activity levels of NQO1, relate to NQO1 activities in mice and man, either without or upon induction of the enzyme by butylated hydroxyanisol (BHA) or indole-3-carbinol (I
Original languageEnglish
Pages (from-to)594-600
JournalToxicology in Vitro
Volume20
Issue number5
DOIs
Publication statusPublished - 2006

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