In vivo subdiffuse scanning laser oximetry of the human retina

Research output: Contribution to JournalArticleAcademicpeer-review

Abstract

Scanning laser ophthalmoscopes (SLOs) have the potential to perform high speed, high contrast, functional imaging of the human retina for diagnosis and follow-up of retinal diseases. Commercial SLOs typically use a monochromatic laser source or a superluminescent diode for imaging. Multispectral SLOs using an array of laser sources for spectral imaging have been demonstrated in research settings, with applications mainly aiming at retinal oxygenation measurements. Previous SLO-based oximetry techniques are predominantly based on wavelengths that depend on laser source availability. We describe an SLO system based on a supercontinuum (SC) source and a double-clad fiber using the single-mode core for illumination and the larger inner cladding for quasi-confocal detection to increase throughput and signal-to-noise ratio. A balanced detection scheme was implemented to suppress the relative intensity noise of the SC source. The SLO produced dual wavelength, high-quality images at 10  frames  /  s with a maximum 20 deg imaging field-of-view with any desired combination of wavelengths in the visible spectrum. We demonstrate SLO-based dual-wavelength oximetry in vessels down to 50  μm in diameter. Reproducibility was demonstrated by performing three different imaging sessions of the same volunteer, 8 min apart. Finally, by performing a wavelength sweep between 485 and 608 nm, we determined, for our SLO geometry, an approximately linear relationship between the effective path length of photons through the blood vessels and the vessel diameter.

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Original languageEnglish
Article number096009
Pages (from-to)1-14
Number of pages14
JournalJournal of biomedical optics
Volume24
Issue number9
DOIs
Publication statusPublished - 1 Sep 2019

Fingerprint

oximetry
retina
Scanning
scanning
Lasers
lasers
Imaging techniques
Wavelength
Light sources
wavelengths
vessels
Oxygenation
oxygenation
blood vessels
noise intensity
Blood vessels
visible spectrum
Image quality
field of view
availability

Keywords

  • hemoglobin
  • hyperspectral imaging
  • oxygen saturation
  • path length
  • retinal oximetry
  • scanning laser ophthalmoscope

Cite this

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title = "In vivo subdiffuse scanning laser oximetry of the human retina",
abstract = "Scanning laser ophthalmoscopes (SLOs) have the potential to perform high speed, high contrast, functional imaging of the human retina for diagnosis and follow-up of retinal diseases. Commercial SLOs typically use a monochromatic laser source or a superluminescent diode for imaging. Multispectral SLOs using an array of laser sources for spectral imaging have been demonstrated in research settings, with applications mainly aiming at retinal oxygenation measurements. Previous SLO-based oximetry techniques are predominantly based on wavelengths that depend on laser source availability. We describe an SLO system based on a supercontinuum (SC) source and a double-clad fiber using the single-mode core for illumination and the larger inner cladding for quasi-confocal detection to increase throughput and signal-to-noise ratio. A balanced detection scheme was implemented to suppress the relative intensity noise of the SC source. The SLO produced dual wavelength, high-quality images at 10  frames  /  s with a maximum 20 deg imaging field-of-view with any desired combination of wavelengths in the visible spectrum. We demonstrate SLO-based dual-wavelength oximetry in vessels down to 50  μm in diameter. Reproducibility was demonstrated by performing three different imaging sessions of the same volunteer, 8 min apart. Finally, by performing a wavelength sweep between 485 and 608 nm, we determined, for our SLO geometry, an approximately linear relationship between the effective path length of photons through the blood vessels and the vessel diameter..",
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In vivo subdiffuse scanning laser oximetry of the human retina. / Damodaran, Mathi; Amelink, Arjen; Feroldi, Fabio; Lochocki, Benjamin; Davidoiu, Valentina; de Boer, Johannes F.

In: Journal of biomedical optics, Vol. 24, No. 9, 096009, 01.09.2019, p. 1-14.

Research output: Contribution to JournalArticleAcademicpeer-review

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T1 - In vivo subdiffuse scanning laser oximetry of the human retina

AU - Damodaran, Mathi

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AU - Feroldi, Fabio

AU - Lochocki, Benjamin

AU - Davidoiu, Valentina

AU - de Boer, Johannes F.

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