Incoherent manipulation of the photoactive yellow protein photocycle with dispersed pump-dump-probe spectroscopy

D.S. Larsen, I.H.M. van Stokkum, M. Vengris, M.A. Horst, F.L. de Weerd, K.J. Hellingwerf, R. van Grondelle

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Abstract

Photoactive yellow protein is the protein responsible for initiating the "blue-light vision" of Halorhodospira halophila. The dynamical processes responsible for triggering the photoactive yellow protein photocycle have been disentangled with the use of a novel application of dispersed ultrafast pump-dump-probe spectroscopy, where the photocycle can be started and interrupted with appropriately tuned and timed laser pulses. This "incoherent" manipulation of the photocycle allows for the detailed spectroscopic investigation of the underlying photocycle dynamics and the construction of a fully self-consistent dynamical model. This model requires three kinetically distinct excited-state intermediates, two (ground-state) photocycle intermediates, I
Original languageEnglish
Pages (from-to)1858-1872
JournalBiophysical Journal
Volume87
Issue number3
DOIs
Publication statusPublished - 2004

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Incoherent manipulation of the photoactive yellow protein photocycle with dispersed pump-dump-probe spectroscopy

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