Incorporation of NGR1 promotes bone regeneration of injectable HA/nHAp hydrogels by anti-inflammation regulation via a MAPK/ERK signaling pathway

Yi Liu; Yifan Zhang; Zexiang Zheng; Wenchao Zhong; Haiyang Wang; Zhen Lin; Lihua Li; Gang Wu

doi:10.3389/fbioe.2022.992961

Incorporation of NGR1 promotes bone regeneration of injectable HA/nHAp hydrogels by anti-inflammation regulation via a MAPK/ERK signaling pathway

Yi Liu
, Yifan Zhang
, Zexiang Zheng
, Wenchao Zhong
, Haiyang Wang
, Zhen Lin
, Lihua Li^*
, Gang Wu

^*Corresponding author for this work

Research output: Contribution to Journal › Article › Academic › peer-review

Abstract

Suitable bone grafts are commonly required to achieve successful bone regeneration, wherein much effort has been spent to optimize their osteogenesis. Increasing evidence has demonstrated that reducing the levels of TNF-α can enhance bone regeneration at the injury site. Notoginsenoside R1 (NGR1) has been extensively studied in the field of anti-inflammation and regenerative medicine. Nanosized hydroxyapatite (nHAp) possesses excellent biocompatibility and osteoconductivity. In this study, we fabricated a thermoresponsive, injectable hyaluronic acid/nHAp (HA/nHAp) composite hydrogel incorporated with NGR1 to promote bone regeneration. Furthermore, NGR1-HA/nHAp hydrogel could enhance bone regeneration than those of HA and HA/nHAp hydrogels, profited by the underlying osteoblastic mechanism that NGR1 could facilitate activation of the MAPK/ERK signaling pathway and down-regulate the expression of TNF-α, ultimately upregulated expression of osteogenic genes. In summary, the NGR1-HA/nHAp composite hydrogel with controlled inflammation, and excellent osteogenic effect, will have great potential for use in bone regeneration applications.

Original language	English
Article number	992961
Pages (from-to)	1-14
Number of pages	14
Journal	Frontiers in Bioengineering and Biotechnology
Volume	10
DOIs	https://doi.org/10.3389/fbioe.2022.992961
Publication status	Published - 23 Sept 2022

Bibliographical note

Funding Information:
This study was financially supported by National Key Research and Development Project (Grant No. 2019YFD0901905), Medical Science and Technology Research fund of Guangdong Province (20181025144221250, 20191A011109, and 2021A1515011254), Scientific Research Project in Universities of Guangzhou Education Burea (201831814), the fund of Chinese Medicine of Guangdong Province (20191216), Guangzhou Health and Family Planning Technology Project (A2019500), Science and Technology Research fund of Guangzhou (202102020796 and 202102020797), Traditional Chinese medicine and integrated Medicine Project of Guangzhou (20222A010052).

Publisher Copyright:
Copyright © 2022 Liu, Zhang, Zheng, Zhong, Wang, Lin, Li and Wu.

Funding

This study was financially supported by National Key Research and Development Project (Grant No. 2019YFD0901905), Medical Science and Technology Research fund of Guangdong Province (20181025144221250, 20191A011109, and 2021A1515011254), Scientific Research Project in Universities of Guangzhou Education Burea (201831814), the fund of Chinese Medicine of Guangdong Province (20191216), Guangzhou Health and Family Planning Technology Project (A2019500), Science and Technology Research fund of Guangzhou (202102020796 and 202102020797), Traditional Chinese medicine and integrated Medicine Project of Guangzhou (20222A010052).

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

SDG 3 Good Health and Well-being

Keywords

bone regeneration
hyaluronic acid
injectable
nanosized hydroxyapatite
notoginsenoside R1

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10.3389/fbioe.2022.992961

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Cite this

@article{007046bd37704180997d089ecea45bc0,

title = "Incorporation of NGR1 promotes bone regeneration of injectable HA/nHAp hydrogels by anti-inflammation regulation via a MAPK/ERK signaling pathway",

abstract = "Suitable bone grafts are commonly required to achieve successful bone regeneration, wherein much effort has been spent to optimize their osteogenesis. Increasing evidence has demonstrated that reducing the levels of TNF-α can enhance bone regeneration at the injury site. Notoginsenoside R1 (NGR1) has been extensively studied in the field of anti-inflammation and regenerative medicine. Nanosized hydroxyapatite (nHAp) possesses excellent biocompatibility and osteoconductivity. In this study, we fabricated a thermoresponsive, injectable hyaluronic acid/nHAp (HA/nHAp) composite hydrogel incorporated with NGR1 to promote bone regeneration. Furthermore, NGR1-HA/nHAp hydrogel could enhance bone regeneration than those of HA and HA/nHAp hydrogels, profited by the underlying osteoblastic mechanism that NGR1 could facilitate activation of the MAPK/ERK signaling pathway and down-regulate the expression of TNF-α, ultimately upregulated expression of osteogenic genes. In summary, the NGR1-HA/nHAp composite hydrogel with controlled inflammation, and excellent osteogenic effect, will have great potential for use in bone regeneration applications.",

keywords = "bone regeneration, hyaluronic acid, injectable, nanosized hydroxyapatite, notoginsenoside R1",

author = "Yi Liu and Yifan Zhang and Zexiang Zheng and Wenchao Zhong and Haiyang Wang and Zhen Lin and Lihua Li and Gang Wu",

note = "Funding Information: This study was financially supported by National Key Research and Development Project (Grant No. 2019YFD0901905), Medical Science and Technology Research fund of Guangdong Province (20181025144221250, 20191A011109, and 2021A1515011254), Scientific Research Project in Universities of Guangzhou Education Burea (201831814), the fund of Chinese Medicine of Guangdong Province (20191216), Guangzhou Health and Family Planning Technology Project (A2019500), Science and Technology Research fund of Guangzhou (202102020796 and 202102020797), Traditional Chinese medicine and integrated Medicine Project of Guangzhou (20222A010052). Publisher Copyright: Copyright {\textcopyright} 2022 Liu, Zhang, Zheng, Zhong, Wang, Lin, Li and Wu.",

year = "2022",

month = sep,

day = "23",

doi = "10.3389/fbioe.2022.992961",

language = "English",

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T1 - Incorporation of NGR1 promotes bone regeneration of injectable HA/nHAp hydrogels by anti-inflammation regulation via a MAPK/ERK signaling pathway

AU - Liu, Yi

AU - Zhang, Yifan

AU - Zheng, Zexiang

AU - Zhong, Wenchao

AU - Wang, Haiyang

AU - Lin, Zhen

AU - Li, Lihua

AU - Wu, Gang

N1 - Funding Information: This study was financially supported by National Key Research and Development Project (Grant No. 2019YFD0901905), Medical Science and Technology Research fund of Guangdong Province (20181025144221250, 20191A011109, and 2021A1515011254), Scientific Research Project in Universities of Guangzhou Education Burea (201831814), the fund of Chinese Medicine of Guangdong Province (20191216), Guangzhou Health and Family Planning Technology Project (A2019500), Science and Technology Research fund of Guangzhou (202102020796 and 202102020797), Traditional Chinese medicine and integrated Medicine Project of Guangzhou (20222A010052). Publisher Copyright: Copyright © 2022 Liu, Zhang, Zheng, Zhong, Wang, Lin, Li and Wu.

PY - 2022/9/23

Y1 - 2022/9/23

N2 - Suitable bone grafts are commonly required to achieve successful bone regeneration, wherein much effort has been spent to optimize their osteogenesis. Increasing evidence has demonstrated that reducing the levels of TNF-α can enhance bone regeneration at the injury site. Notoginsenoside R1 (NGR1) has been extensively studied in the field of anti-inflammation and regenerative medicine. Nanosized hydroxyapatite (nHAp) possesses excellent biocompatibility and osteoconductivity. In this study, we fabricated a thermoresponsive, injectable hyaluronic acid/nHAp (HA/nHAp) composite hydrogel incorporated with NGR1 to promote bone regeneration. Furthermore, NGR1-HA/nHAp hydrogel could enhance bone regeneration than those of HA and HA/nHAp hydrogels, profited by the underlying osteoblastic mechanism that NGR1 could facilitate activation of the MAPK/ERK signaling pathway and down-regulate the expression of TNF-α, ultimately upregulated expression of osteogenic genes. In summary, the NGR1-HA/nHAp composite hydrogel with controlled inflammation, and excellent osteogenic effect, will have great potential for use in bone regeneration applications.

AB - Suitable bone grafts are commonly required to achieve successful bone regeneration, wherein much effort has been spent to optimize their osteogenesis. Increasing evidence has demonstrated that reducing the levels of TNF-α can enhance bone regeneration at the injury site. Notoginsenoside R1 (NGR1) has been extensively studied in the field of anti-inflammation and regenerative medicine. Nanosized hydroxyapatite (nHAp) possesses excellent biocompatibility and osteoconductivity. In this study, we fabricated a thermoresponsive, injectable hyaluronic acid/nHAp (HA/nHAp) composite hydrogel incorporated with NGR1 to promote bone regeneration. Furthermore, NGR1-HA/nHAp hydrogel could enhance bone regeneration than those of HA and HA/nHAp hydrogels, profited by the underlying osteoblastic mechanism that NGR1 could facilitate activation of the MAPK/ERK signaling pathway and down-regulate the expression of TNF-α, ultimately upregulated expression of osteogenic genes. In summary, the NGR1-HA/nHAp composite hydrogel with controlled inflammation, and excellent osteogenic effect, will have great potential for use in bone regeneration applications.

KW - bone regeneration

KW - hyaluronic acid

KW - injectable

KW - nanosized hydroxyapatite

KW - notoginsenoside R1

UR - https://www.scopus.com/pages/publications/85140054224

UR - https://www.scopus.com/pages/publications/85140054224#tab=citedBy

U2 - 10.3389/fbioe.2022.992961

DO - 10.3389/fbioe.2022.992961

M3 - Article

AN - SCOPUS:85140054224

SN - 2296-4185

VL - 10

SP - 1

EP - 14

JO - Frontiers in Bioengineering and Biotechnology

JF - Frontiers in Bioengineering and Biotechnology

M1 - 992961

ER -

Incorporation of NGR1 promotes bone regeneration of injectable HA/nHAp hydrogels by anti-inflammation regulation via a MAPK/ERK signaling pathway

Abstract

Bibliographical note

Funding

UN SDGs

Keywords

Access to Document

Persistent URL (handle)

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