TY - JOUR
T1 - Influence of adaptive evolution of cadmium tolerance on neutral and functional genetic variation in Orchesella cincta
AU - Costa, D.M.
AU - Mariën, Janine
AU - Janssens, Thierry K S
AU - van Gestel, Cornelis A M
AU - Driessen, Gerard
AU - Sousa, José Paulo
AU - van Straalen, Nico M
AU - Roelofs, Dick
PY - 2012/10
Y1 - 2012/10
N2 - Adaptation to environmental toxicants, such as metals, can affect population genetic diversity, both at neutral and selectable loci. At the transcriptional level, evolution of metal tolerance is possible due to the existence of polymorphisms in the cis-regulatory sequences of stressresponsive genes such as the metallothionein gene (mt). This study investigated the influence of cadmium adaptation on genetic diversity of soil-living Orchesella cincta (Collembola) populations in neutral (microsatellites and AFLP) and in functional (mt promoter) markers. Also, the influence of cis- and trans-acting factors on increased tolerance was addressed. No reduced genetic diversity was observed in two tolerant populations compared to five sensitive populations, either in neutral or in selectable markers. Extensive migration along with a large population size may explain the high genetic diversity measured. The metal-tolerant phenotype seems to be mostly influenced by genetic factors acting in cis on mt gene expression. The results suggest that certain promoter genotypes, which are found mainly or exclusively in Cd-tolerant populations, contribute to higher constitutive mt gene expression in individuals from these populations. However, more studies are needed to clearly unravel the influence of cis/transregulatory evolution in tolerant populations. © Springer Science+Business Media, LLC 2012.
AB - Adaptation to environmental toxicants, such as metals, can affect population genetic diversity, both at neutral and selectable loci. At the transcriptional level, evolution of metal tolerance is possible due to the existence of polymorphisms in the cis-regulatory sequences of stressresponsive genes such as the metallothionein gene (mt). This study investigated the influence of cadmium adaptation on genetic diversity of soil-living Orchesella cincta (Collembola) populations in neutral (microsatellites and AFLP) and in functional (mt promoter) markers. Also, the influence of cis- and trans-acting factors on increased tolerance was addressed. No reduced genetic diversity was observed in two tolerant populations compared to five sensitive populations, either in neutral or in selectable markers. Extensive migration along with a large population size may explain the high genetic diversity measured. The metal-tolerant phenotype seems to be mostly influenced by genetic factors acting in cis on mt gene expression. The results suggest that certain promoter genotypes, which are found mainly or exclusively in Cd-tolerant populations, contribute to higher constitutive mt gene expression in individuals from these populations. However, more studies are needed to clearly unravel the influence of cis/transregulatory evolution in tolerant populations. © Springer Science+Business Media, LLC 2012.
KW - Amplified Fragment Length Polymorphism Analysis
KW - Animals
KW - Arthropods
KW - Biological Evolution
KW - Cadmium
KW - Genetic Variation
KW - Genotype
KW - Metallothionein
KW - Microsatellite Repeats
KW - Phenotype
KW - Promoter Regions, Genetic
KW - RNA, Messenger
KW - Journal Article
KW - Research Support, Non-U.S. Gov't
UR - https://www.scopus.com/pages/publications/84868156565
UR - https://www.scopus.com/inward/citedby.url?scp=84868156565&partnerID=8YFLogxK
U2 - 10.1007/s10646-012-0961-9
DO - 10.1007/s10646-012-0961-9
M3 - Article
C2 - 22717685
SN - 0963-9292
VL - 21
SP - 2078
EP - 2087
JO - Ecotoxicology
JF - Ecotoxicology
IS - 7
ER -