@inbook{8ca3981011ae4ff8afd290fd3d524a76,
title = "Integrated immunoprecipitation: Blue native gel electrophoresis—mass spectrometry for the identification of protein subcomplexes",
abstract = "Proteins typically act as components of poly-protein complexes, which may vary in composition and function, and may depend on subcellular localization and cellular state. To understand their biology, there is a need to identify the constituents in these various protein complexes. Conventional immunoprecipitation combined with proteomics analysis is nowadays routinely used to identify multiple interactors of a bait protein; however, it is unable to distinguish protein subcomplexes from one another. Blue native polyacrylamide gel electrophoresis is appropriate to separate individual protein complexes. Here, we describe a protocol that combines the specificity of immunoprecipitation, the size separating capacity of Blue native polyacrylamide gel electrophoresis, and subsequent mass spectrometry to delineate the protein constituents in subcomplexes of a targeted protein.",
keywords = "Blue native polyacrylamide gel electrophoresis, Immunoprecipitation, Mass spectrometry, Protein complexes, Proteomics",
author = "{van der Spek}, {Sophie J.F.} and Smit, {August B.} and Pandya, {Nikhil J.}",
year = "2019",
doi = "10.1007/978-1-4939-9662-9_8",
language = "English",
isbn = "9781493996612",
series = "Neuromethods book series (NM)",
publisher = "Humana Press Inc.",
pages = "85--94",
editor = "Li, {Ka Wan}",
booktitle = "Neuroproteomics",
edition = "2nd",
}