Interaction between the photoprotective protein LHCSR3 and C2S2 Photosystem II supercomplex in Chlamydomonas reinhardtii

Dmitriy A. Semchonok, K. N. Sathish Yadav, Pengqi Xu, Bartlomiej Drop, Roberta Croce, Egbert J. Boekema

Research output: Contribution to JournalArticleAcademicpeer-review

Abstract

Photosynthetic organisms can thermally dissipate excess of absorbed energy in high-light conditions in a process known as non-photochemical quenching (NPQ). In the green alga Chlamydomonas reinhardtii this process depends on the presence of the light-harvesting protein LHCSR3, which is only expressed in high light. LHCSR3 has been shown to act as a quencher when associated with the Photosystem II supercomplex and to respond to pH changes, but the mechanism of quenching has not been elucidated yet. In this work we have studied the interaction between LHCSR3 and Photosystem II C2S2 supercomplexes by single particle electron microscopy. It was found that LHCSR3 predominantly binds at three different positions and that the CP26 subunit and the LHCII trimer of C2S2 supercomplexes are involved in binding, while we could not find evidences for a direct association of LHCSR3 with the PSII core. At all three locations LHCSR3 is present almost exclusively as a dimer.

Original languageEnglish
Pages (from-to)379-385
Number of pages7
JournalBiochimica et Biophysica Acta (BBA) - Bioenergetics
Volume1858
Issue number5
DOIs
Publication statusPublished - 1 May 2017

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Chlamydomonas reinhardtii
Photosystem II Protein Complex
Light
Quenching
Chlorophyta
Proteins
Algae
Dimers
Electron microscopy
Electron Microscopy

Keywords

  • Chlamydomonas reinhardtii
  • Electron
  • LHCSR3
  • Microscopy
  • Photosystem I

Cite this

Semchonok, Dmitriy A. ; Sathish Yadav, K. N. ; Xu, Pengqi ; Drop, Bartlomiej ; Croce, Roberta ; Boekema, Egbert J. / Interaction between the photoprotective protein LHCSR3 and C2S2 Photosystem II supercomplex in Chlamydomonas reinhardtii. In: Biochimica et Biophysica Acta (BBA) - Bioenergetics. 2017 ; Vol. 1858, No. 5. pp. 379-385.
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Interaction between the photoprotective protein LHCSR3 and C2S2 Photosystem II supercomplex in Chlamydomonas reinhardtii. / Semchonok, Dmitriy A.; Sathish Yadav, K. N.; Xu, Pengqi; Drop, Bartlomiej; Croce, Roberta; Boekema, Egbert J.

In: Biochimica et Biophysica Acta (BBA) - Bioenergetics, Vol. 1858, No. 5, 01.05.2017, p. 379-385.

Research output: Contribution to JournalArticleAcademicpeer-review

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AU - Drop, Bartlomiej

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