Interaction of extracellular vesicles with Si surface studied by nanomechanical microcantilever sensors

S. Federici; A. Ridolfi; A. Zendrini; A. Radeghieri; E. Bontempi; L.E. Depero; P. Bergese

doi:10.3390/app8030404

Interaction of extracellular vesicles with Si surface studied by nanomechanical microcantilever sensors

S. Federici, A. Ridolfi, A. Zendrini, A. Radeghieri, E. Bontempi, L.E. Depero, P. Bergese

Research output: Contribution to Journal › Article › Academic › peer-review

Abstract

© 2018 by the authors.We report on the interaction of small (> 150 nm) extracellular vesicles (EVs) with silicon surface. The study is conducted by leveraging Si nanomechanical microcantilever sensors actuated in static and dynamic modes, that allow tracking of EV collective adsorption energy and adsorbed mass. Upon incubation for 30 min at about 10 nM concentration, EVs isolated from human vascular endothelial cell (HVEC) lines form a patchy layer that partially covers the Si total surface. Formation of this layer releases a surface energy equal to (8 ± 1) mJ/m2, typical of weak electrostatic interactions. These findings give a first insight into the EV-Si interface and proof the possibility to realize new hybrid biointerphases that can be exploited as advanced models to investigate properties of biological membranes and/or biosensing platforms that take advantage of biomolecules embedded/supported in membranes.

Original language	English
Article number	404
Journal	Applied Sciences (Switzerland)
Volume	8
Issue number	3
DOIs	https://doi.org/10.3390/app8030404
Publication status	Published - 9 Mar 2018
Externally published	Yes

Funding

Acknowledgments: The authors acknowledge the funding of the Italian MIUR (Ministero dell’Istruzione, dell’Università e della Ricerca) for financial support of ex 60%. They also wish to thank Debora Berti and Costanza Montis for fruitful discussions, and Orietta Salvucci for supplying HVEC cell lines.

Funders	Funder number
Ministero dell’Istruzione, dell’Università e della Ricerca

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title = "Interaction of extracellular vesicles with Si surface studied by nanomechanical microcantilever sensors",

abstract = "{\textcopyright} 2018 by the authors.We report on the interaction of small (> 150 nm) extracellular vesicles (EVs) with silicon surface. The study is conducted by leveraging Si nanomechanical microcantilever sensors actuated in static and dynamic modes, that allow tracking of EV collective adsorption energy and adsorbed mass. Upon incubation for 30 min at about 10 nM concentration, EVs isolated from human vascular endothelial cell (HVEC) lines form a patchy layer that partially covers the Si total surface. Formation of this layer releases a surface energy equal to (8 ± 1) mJ/m2, typical of weak electrostatic interactions. These findings give a first insight into the EV-Si interface and proof the possibility to realize new hybrid biointerphases that can be exploited as advanced models to investigate properties of biological membranes and/or biosensing platforms that take advantage of biomolecules embedded/supported in membranes.",

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AU - Federici, S.

AU - Ridolfi, A.

AU - Zendrini, A.

AU - Radeghieri, A.

AU - Bontempi, E.

AU - Depero, L.E.

AU - Bergese, P.

PY - 2018/3/9

Y1 - 2018/3/9

N2 - © 2018 by the authors.We report on the interaction of small (> 150 nm) extracellular vesicles (EVs) with silicon surface. The study is conducted by leveraging Si nanomechanical microcantilever sensors actuated in static and dynamic modes, that allow tracking of EV collective adsorption energy and adsorbed mass. Upon incubation for 30 min at about 10 nM concentration, EVs isolated from human vascular endothelial cell (HVEC) lines form a patchy layer that partially covers the Si total surface. Formation of this layer releases a surface energy equal to (8 ± 1) mJ/m2, typical of weak electrostatic interactions. These findings give a first insight into the EV-Si interface and proof the possibility to realize new hybrid biointerphases that can be exploited as advanced models to investigate properties of biological membranes and/or biosensing platforms that take advantage of biomolecules embedded/supported in membranes.

AB - © 2018 by the authors.We report on the interaction of small (> 150 nm) extracellular vesicles (EVs) with silicon surface. The study is conducted by leveraging Si nanomechanical microcantilever sensors actuated in static and dynamic modes, that allow tracking of EV collective adsorption energy and adsorbed mass. Upon incubation for 30 min at about 10 nM concentration, EVs isolated from human vascular endothelial cell (HVEC) lines form a patchy layer that partially covers the Si total surface. Formation of this layer releases a surface energy equal to (8 ± 1) mJ/m2, typical of weak electrostatic interactions. These findings give a first insight into the EV-Si interface and proof the possibility to realize new hybrid biointerphases that can be exploited as advanced models to investigate properties of biological membranes and/or biosensing platforms that take advantage of biomolecules embedded/supported in membranes.

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DO - 10.3390/app8030404

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JO - Applied Sciences (Switzerland)

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Interaction of extracellular vesicles with Si surface studied by nanomechanical microcantilever sensors

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