Interaction proteomics reveals brain region-specific AMPA receptor complexes

N. Chen; N.J. Pandya; F.T.W. Koopmans; V. Castelo-Szekelv; R.C. van der Schors; A.B. Smit; K.W. Li

doi:10.1021/pr500697b

Interaction proteomics reveals brain region-specific AMPA receptor complexes

N. Chen, N.J. Pandya, F.T.W. Koopmans, V. Castelo-Szekelv, R.C. van der Schors, A.B. Smit, K.W. Li

Research output: Contribution to Journal › Article › Academic › peer-review

Abstract

Fast excitatory synaptic transmission in the brain is mediated by glutamate acting on postsynaptic AMPA receptors. Recent studies have revealed a substantial number of AMPA receptor auxiliary proteins, which potentially contribute to the regulation of AMPA receptor trafficking, subcellular receptor localization, and receptor gating properties. Here we examined the AMPA receptor interactomes from cortex, hippocampus, and cerebellum by comprehensive interaction proteomics. The study reveals that AMPA receptor auxiliary proteins are engaged in distinct brain region-specific AMPA receptors subcomplexes, which might underlie brain region-specific differential regulation of AMPA receptor properties. Depending on the brain region, an interacting protein can be involved in an AMPA and a non-AMPA receptor complex.

Original language	English
Pages (from-to)	5695-5706
Journal	Journal of Proteome Research
Volume	13
Issue number	12
DOIs	https://doi.org/10.1021/pr500697b
Publication status	Published - 2014

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title = "Interaction proteomics reveals brain region-specific AMPA receptor complexes",

abstract = "Fast excitatory synaptic transmission in the brain is mediated by glutamate acting on postsynaptic AMPA receptors. Recent studies have revealed a substantial number of AMPA receptor auxiliary proteins, which potentially contribute to the regulation of AMPA receptor trafficking, subcellular receptor localization, and receptor gating properties. Here we examined the AMPA receptor interactomes from cortex, hippocampus, and cerebellum by comprehensive interaction proteomics. The study reveals that AMPA receptor auxiliary proteins are engaged in distinct brain region-specific AMPA receptors subcomplexes, which might underlie brain region-specific differential regulation of AMPA receptor properties. Depending on the brain region, an interacting protein can be involved in an AMPA and a non-AMPA receptor complex.",

author = "N. Chen and N.J. Pandya and F.T.W. Koopmans and V. Castelo-Szekelv and {van der Schors}, R.C. and A.B. Smit and K.W. Li",

year = "2014",

doi = "10.1021/pr500697b",

language = "English",

volume = "13",

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journal = "Journal of Proteome Research",

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T1 - Interaction proteomics reveals brain region-specific AMPA receptor complexes

AU - Chen, N.

AU - Pandya, N.J.

AU - Koopmans, F.T.W.

AU - Castelo-Szekelv, V.

AU - van der Schors, R.C.

AU - Smit, A.B.

AU - Li, K.W.

PY - 2014

Y1 - 2014

N2 - Fast excitatory synaptic transmission in the brain is mediated by glutamate acting on postsynaptic AMPA receptors. Recent studies have revealed a substantial number of AMPA receptor auxiliary proteins, which potentially contribute to the regulation of AMPA receptor trafficking, subcellular receptor localization, and receptor gating properties. Here we examined the AMPA receptor interactomes from cortex, hippocampus, and cerebellum by comprehensive interaction proteomics. The study reveals that AMPA receptor auxiliary proteins are engaged in distinct brain region-specific AMPA receptors subcomplexes, which might underlie brain region-specific differential regulation of AMPA receptor properties. Depending on the brain region, an interacting protein can be involved in an AMPA and a non-AMPA receptor complex.

AB - Fast excitatory synaptic transmission in the brain is mediated by glutamate acting on postsynaptic AMPA receptors. Recent studies have revealed a substantial number of AMPA receptor auxiliary proteins, which potentially contribute to the regulation of AMPA receptor trafficking, subcellular receptor localization, and receptor gating properties. Here we examined the AMPA receptor interactomes from cortex, hippocampus, and cerebellum by comprehensive interaction proteomics. The study reveals that AMPA receptor auxiliary proteins are engaged in distinct brain region-specific AMPA receptors subcomplexes, which might underlie brain region-specific differential regulation of AMPA receptor properties. Depending on the brain region, an interacting protein can be involved in an AMPA and a non-AMPA receptor complex.

U2 - 10.1021/pr500697b

DO - 10.1021/pr500697b

M3 - Article

SN - 1535-3893

VL - 13

SP - 5695

EP - 5706

JO - Journal of Proteome Research

JF - Journal of Proteome Research

IS - 12

ER -

Interaction proteomics reveals brain region-specific AMPA receptor complexes

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