Interdependence of PKC-dependent and PKC-independent pathways for presynaptic plasticity

K.D.B. Wierda, R.F.G. Toonen, H. de Wit, A.B. Brussaard, M. Verhage

Research output: Contribution to JournalArticleAcademicpeer-review

Abstract

Diacylglycerol (DAG) is a prominent endogenous modulator of synaptic transmission. Recent studies proposed two apparently incompatible pathways, via protein kinase C (PKC) and via Munc13. Here we show how these two pathways converge. First, we confirm that DAG analogs indeed continue to potentiate transmission after PKC inhibition (the Munc13 pathway), but only in neurons that previously experienced DAG analogs, before PKC inhibition started. Second, we identify an essential PKC pathway by expressing a PKC-insensitive Munc18-1 mutant in munc18-1 null mutant neurons. This mutant supported basic transmission, but not DAG-induced potentiation and vesicle redistribution. Moreover, synaptic depression was increased, but not Ca
Original languageEnglish
Pages (from-to)275-290
JournalNeuron
Volume54
Issue number2
DOIs
Publication statusPublished - 2007

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