TY - JOUR
T1 - Interdependence of PKC-dependent and PKC-independent pathways for presynaptic plasticity
AU - Wierda, K.D.B.
AU - Toonen, R.F.G.
AU - de Wit, H.
AU - Brussaard, A.B.
AU - Verhage, M.
PY - 2007
Y1 - 2007
N2 - Diacylglycerol (DAG) is a prominent endogenous modulator of synaptic transmission. Recent studies proposed two apparently incompatible pathways, via protein kinase C (PKC) and via Munc13. Here we show how these two pathways converge. First, we confirm that DAG analogs indeed continue to potentiate transmission after PKC inhibition (the Munc13 pathway), but only in neurons that previously experienced DAG analogs, before PKC inhibition started. Second, we identify an essential PKC pathway by expressing a PKC-insensitive Munc18-1 mutant in munc18-1 null mutant neurons. This mutant supported basic transmission, but not DAG-induced potentiation and vesicle redistribution. Moreover, synaptic depression was increased, but not Ca
AB - Diacylglycerol (DAG) is a prominent endogenous modulator of synaptic transmission. Recent studies proposed two apparently incompatible pathways, via protein kinase C (PKC) and via Munc13. Here we show how these two pathways converge. First, we confirm that DAG analogs indeed continue to potentiate transmission after PKC inhibition (the Munc13 pathway), but only in neurons that previously experienced DAG analogs, before PKC inhibition started. Second, we identify an essential PKC pathway by expressing a PKC-insensitive Munc18-1 mutant in munc18-1 null mutant neurons. This mutant supported basic transmission, but not DAG-induced potentiation and vesicle redistribution. Moreover, synaptic depression was increased, but not Ca
U2 - 10.1016/j.neuron.2007.04.001
DO - 10.1016/j.neuron.2007.04.001
M3 - Article
VL - 54
SP - 275
EP - 290
JO - Neuron
JF - Neuron
SN - 0896-6273
IS - 2
ER -
