Background: Internet-based screening for depression is becoming increasingly important. The aim of this study is to validate the Edinburgh Depression Scale (EDS) for internet administration. Methods: In 407 participants (64% women; 36% men) with subthreshold depression (mean age = 55 years; S.D. = 4.9) positive predictive values for a syndromal CIDI diagnosis of clinical depression were calculated and compared to those from paper and pencil validation studies. At one-year follow-up, internal consistency and convergent validity of the internet-based EDS were determined in 177 participants by Cronbach's alpha and correlations with the internet-administered BDI and SCL-90 subscales depression and anxiety. Results: Positive predictive values ranged between 29% and 33% at cut-off scores of 12 to 14. Cronbach's alpha for the internet-administered EDS was 0.87. The EDS correlated significantly with the internet-administered BDI (r = .75; p < .001) and two internet-administered subscales of the SCL-90: depression (r = .77; p < .001) and anxiety (r = .72; p < .001). A major limitation is that the study was conducted without a control group of healthy subjects. Conclusion: The psychometric properties of the internet-administered EDS are comparable to those of the paper and pencil EDS. © 2007 Elsevier B.V. All rights reserved.