Interplay between Ciliary Ultrastructure and IFT-Train Dynamics Revealed by Single-Molecule Super-resolution Imaging

Felix Oswald, Bram Prevo, Seyda Acar, Erwin J.G. Peterman

Research output: Contribution to JournalArticleAcademicpeer-review

Abstract

Cilia are built and maintained by intraflagellar transport (IFT), driving IFT trains back and forth along the ciliary axoneme. How IFT brings about the intricate ciliary structure and how this structure affects IFT are not well understood. We identify, using single-molecule super-resolution imaging of IFT components in living C. elegans, ciliary subdomains, enabling correlation of IFT-train dynamics to ciliary ultra-structure. In the transition zone, IFT dynamics are impaired, resulting in frequent pauses. At the ciliary base and tip, IFT trains show intriguing turnaround dynamics. Surprisingly, deletion of IFT motor kinesin-II not only affects IFT-train dynamics but also alters ciliary structure. Super-resolution imaging in these mutant animals suggests that the arrangement of IFT trains with respect to the axonemal microtubules is different than in wild-type animals. Our results reveal a complex, mutual interplay between ciliary ultrastructure and IFT-train dynamics, highlighting the importance of physical cues in the control of IFT dynamics. Oswald et al. use trajectories of single intraflagellar transport proteins in the chemosensory cilia of C. elegans to generate super-resolution fluorescence maps. Local motility properties can be correlated to ciliary ultrastructure. They find that in the absence of kinesin-II, the ciliary ultrastructure is substantially altered.

Original languageEnglish
Pages (from-to)224-235
Number of pages12
JournalCell Reports
Volume25
Issue number1
Early online date2 Oct 2018
DOIs
Publication statusPublished - 2 Oct 2018

Fingerprint

Cilia
Axoneme
Imaging techniques
Molecules
Wild Animals
Microtubules
Cues
Carrier Proteins
Fluorescence
Animals
kinesin-II
Trajectories

Keywords

  • cilia
  • intracellular transport
  • intraflagellar transport
  • molecular motor proteins
  • motor cooperation
  • single-molecule fluorescence microscopy
  • super-resolution microscopy

Cite this

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abstract = "Cilia are built and maintained by intraflagellar transport (IFT), driving IFT trains back and forth along the ciliary axoneme. How IFT brings about the intricate ciliary structure and how this structure affects IFT are not well understood. We identify, using single-molecule super-resolution imaging of IFT components in living C. elegans, ciliary subdomains, enabling correlation of IFT-train dynamics to ciliary ultra-structure. In the transition zone, IFT dynamics are impaired, resulting in frequent pauses. At the ciliary base and tip, IFT trains show intriguing turnaround dynamics. Surprisingly, deletion of IFT motor kinesin-II not only affects IFT-train dynamics but also alters ciliary structure. Super-resolution imaging in these mutant animals suggests that the arrangement of IFT trains with respect to the axonemal microtubules is different than in wild-type animals. Our results reveal a complex, mutual interplay between ciliary ultrastructure and IFT-train dynamics, highlighting the importance of physical cues in the control of IFT dynamics. Oswald et al. use trajectories of single intraflagellar transport proteins in the chemosensory cilia of C. elegans to generate super-resolution fluorescence maps. Local motility properties can be correlated to ciliary ultrastructure. They find that in the absence of kinesin-II, the ciliary ultrastructure is substantially altered.",
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Interplay between Ciliary Ultrastructure and IFT-Train Dynamics Revealed by Single-Molecule Super-resolution Imaging. / Oswald, Felix; Prevo, Bram; Acar, Seyda; Peterman, Erwin J.G.

In: Cell Reports, Vol. 25, No. 1, 02.10.2018, p. 224-235.

Research output: Contribution to JournalArticleAcademicpeer-review

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