Interstrain variability of human vaginal lactobacillus crispatus for metabolism of biogenic amines and antimicrobial activity against urogenital pathogens

S. Puebla-Barragan; E. Watson; C. van der Veer; J.A. Chmiel; C. Carr; J.P. Burton; M. Sumarah; R. Kort; G. Reid

doi:10.3390/molecules26154538

Interstrain variability of human vaginal lactobacillus crispatus for metabolism of biogenic amines and antimicrobial activity against urogenital pathogens

S. Puebla-Barragan, E. Watson, C. van der Veer, J.A. Chmiel, C. Carr, J.P. Burton, M. Sumarah, R. Kort, G. Reid

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Research output: Contribution to Journal › Article › Academic › peer-review

Abstract

© 2021 by the authors. Licensee MDPI, Basel, Switzerland.Lactobacillus crispatus is the dominant species in the vagina of many women. With the potential for strains of this species to be used as a probiotic to help prevent and treat dysbiosis, we investigated isolates from vaginal swabs with Lactobacillus-dominated and a dysbiotic microbiota. A comparative genome analysis led to the identification of metabolic pathways for synthesis and degradation of three major biogenic amines in most strains. However, targeted metabolomic analysis of the production and degradation of biogenic amines showed that certain strains have either the ability to produce or to degrade these compounds. Notably, six strains produced cadaverine, one produced putrescine, and two produced tyramine. These biogenic amines are known to raise vaginal pH, cause malodour, and make the environment more favourable to vaginal pathogens. In vitro experiments confirmed that strains isolated from women with a dysbiotic vaginal microbiota have higher antimicrobial effects against the common urogenital pathogens Escherichia coli and En-terococcus faecium. The results indicate that not all L. crispatus vaginal strains appear suitable for probiotic application and the basis for selection should not be only the overall composition of the vaginal microbiota of the host from which they came, but specific biochemical and genetic traits.

Original language	English
Article number	4538
Journal	Molecules
Volume	26
Issue number	15
DOIs	https://doi.org/10.3390/molecules26154538
Publication status	Published - 1 Aug 2021

Funding

Acknowledgments: This work was supported by an anonymous donor through St. Joseph’s Health Care Foundation. S.P.-B is funded by the Mexican Council of Science and Technology (CONACyT). We acknowledge the invaluable assistance from Shannon Senney, as well as the undergraduate students, Pranav Tandon and Danielle Luskind, who assisted throughout this project, the members of the Reid and Burton labs for their support, Justin Renaud and Stephanie Collins for their metabo-lomics guidance, and collaborators in Amsterdam. S.P.-B is funded by the Mexican Council of Science and Technology (CVU 692895). This work was supported by an anonymous donor through St. Joseph?s Health Care Foundation. S.P.-B is funded by the Mexican Council of Science and Technology (CONACyT). We acknowledge the invaluable assistance from Shannon Senney, as well as the undergraduate stu-dents, Pranav Tandon and Danielle Luskind, who assisted throughout this project, the members of the Reid and Burton labs for their support, Justin Renaud and Stephanie Collins for their metabo-lomics guidance, and collaborators in Amsterdam. Funding: S.P.-B is funded by the Mexican Council of Science and Technology (CVU 692895).

Funders	Funder number
St. Joseph?s Health Care Foundation
St. Joseph’s Health Care Foundation
Consejo Nacional de Ciencia y Tecnología	CVU 692895

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title = "Interstrain variability of human vaginal lactobacillus crispatus for metabolism of biogenic amines and antimicrobial activity against urogenital pathogens",

abstract = "{\textcopyright} 2021 by the authors. Licensee MDPI, Basel, Switzerland.Lactobacillus crispatus is the dominant species in the vagina of many women. With the potential for strains of this species to be used as a probiotic to help prevent and treat dysbiosis, we investigated isolates from vaginal swabs with Lactobacillus-dominated and a dysbiotic microbiota. A comparative genome analysis led to the identification of metabolic pathways for synthesis and degradation of three major biogenic amines in most strains. However, targeted metabolomic analysis of the production and degradation of biogenic amines showed that certain strains have either the ability to produce or to degrade these compounds. Notably, six strains produced cadaverine, one produced putrescine, and two produced tyramine. These biogenic amines are known to raise vaginal pH, cause malodour, and make the environment more favourable to vaginal pathogens. In vitro experiments confirmed that strains isolated from women with a dysbiotic vaginal microbiota have higher antimicrobial effects against the common urogenital pathogens Escherichia coli and En-terococcus faecium. The results indicate that not all L. crispatus vaginal strains appear suitable for probiotic application and the basis for selection should not be only the overall composition of the vaginal microbiota of the host from which they came, but specific biochemical and genetic traits.",

author = "S. Puebla-Barragan and E. Watson and {van der Veer}, C. and J.A. Chmiel and C. Carr and J.P. Burton and M. Sumarah and R. Kort and G. Reid",

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T1 - Interstrain variability of human vaginal lactobacillus crispatus for metabolism of biogenic amines and antimicrobial activity against urogenital pathogens

AU - Puebla-Barragan, S.

AU - Watson, E.

AU - van der Veer, C.

AU - Chmiel, J.A.

AU - Carr, C.

AU - Burton, J.P.

AU - Sumarah, M.

AU - Kort, R.

AU - Reid, G.

PY - 2021/8/1

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N2 - © 2021 by the authors. Licensee MDPI, Basel, Switzerland.Lactobacillus crispatus is the dominant species in the vagina of many women. With the potential for strains of this species to be used as a probiotic to help prevent and treat dysbiosis, we investigated isolates from vaginal swabs with Lactobacillus-dominated and a dysbiotic microbiota. A comparative genome analysis led to the identification of metabolic pathways for synthesis and degradation of three major biogenic amines in most strains. However, targeted metabolomic analysis of the production and degradation of biogenic amines showed that certain strains have either the ability to produce or to degrade these compounds. Notably, six strains produced cadaverine, one produced putrescine, and two produced tyramine. These biogenic amines are known to raise vaginal pH, cause malodour, and make the environment more favourable to vaginal pathogens. In vitro experiments confirmed that strains isolated from women with a dysbiotic vaginal microbiota have higher antimicrobial effects against the common urogenital pathogens Escherichia coli and En-terococcus faecium. The results indicate that not all L. crispatus vaginal strains appear suitable for probiotic application and the basis for selection should not be only the overall composition of the vaginal microbiota of the host from which they came, but specific biochemical and genetic traits.

AB - © 2021 by the authors. Licensee MDPI, Basel, Switzerland.Lactobacillus crispatus is the dominant species in the vagina of many women. With the potential for strains of this species to be used as a probiotic to help prevent and treat dysbiosis, we investigated isolates from vaginal swabs with Lactobacillus-dominated and a dysbiotic microbiota. A comparative genome analysis led to the identification of metabolic pathways for synthesis and degradation of three major biogenic amines in most strains. However, targeted metabolomic analysis of the production and degradation of biogenic amines showed that certain strains have either the ability to produce or to degrade these compounds. Notably, six strains produced cadaverine, one produced putrescine, and two produced tyramine. These biogenic amines are known to raise vaginal pH, cause malodour, and make the environment more favourable to vaginal pathogens. In vitro experiments confirmed that strains isolated from women with a dysbiotic vaginal microbiota have higher antimicrobial effects against the common urogenital pathogens Escherichia coli and En-terococcus faecium. The results indicate that not all L. crispatus vaginal strains appear suitable for probiotic application and the basis for selection should not be only the overall composition of the vaginal microbiota of the host from which they came, but specific biochemical and genetic traits.

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DO - 10.3390/molecules26154538

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JO - Molecules

JF - Molecules

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M1 - 4538

ER -

Interstrain variability of human vaginal lactobacillus crispatus for metabolism of biogenic amines and antimicrobial activity against urogenital pathogens

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