Intracellular manipulation of chromatin using magnetic nanoparticles

Johannes S Kanger; Vinod Subramaniam; Roel van Driel

doi:10.1007/s10577-008-1239-1

Intracellular manipulation of chromatin using magnetic nanoparticles

Johannes S Kanger, Vinod Subramaniam, Roel van Driel

Research output: Contribution to Journal › Review article › Academic › peer-review

Abstract

Magnetic tweezers are widely used for manipulating small magnetic beads inside the cell cytoplasm in order to gain insight into the structural and mechanical properties of the cytoskeleton. Here we discuss the use of magnetic tweezers for the study of nuclear architecture and the mechanical properties of chromatin in living cells. A custom-built, dedicated micro magnetic tweezer set-up is described. We review progress that has been made in applying this technology for the study of chromatin structure and discuss its prospects for the in situ analysis of nuclear architecture and chromatin function.

Original language	English
Pages (from-to)	511-22
Number of pages	12
Journal	Chromosome research : an international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology
Volume	16
Issue number	3
DOIs	https://doi.org/10.1007/s10577-008-1239-1
Publication status	Published - 2008

Keywords

Biomechanical Phenomena
Chromatin
HeLa Cells
Humans
Magnetics
Metal Nanoparticles
Microscopy, Atomic Force
Models, Biological
Nanotechnology
Optical Tweezers
Rheology
Journal Article
Review

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10.1007/s10577-008-1239-1

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title = "Intracellular manipulation of chromatin using magnetic nanoparticles",

abstract = "Magnetic tweezers are widely used for manipulating small magnetic beads inside the cell cytoplasm in order to gain insight into the structural and mechanical properties of the cytoskeleton. Here we discuss the use of magnetic tweezers for the study of nuclear architecture and the mechanical properties of chromatin in living cells. A custom-built, dedicated micro magnetic tweezer set-up is described. We review progress that has been made in applying this technology for the study of chromatin structure and discuss its prospects for the in situ analysis of nuclear architecture and chromatin function.",

keywords = "Biomechanical Phenomena, Chromatin, HeLa Cells, Humans, Magnetics, Metal Nanoparticles, Microscopy, Atomic Force, Models, Biological, Nanotechnology, Optical Tweezers, Rheology, Journal Article, Review",

author = "Kanger, {Johannes S} and Vinod Subramaniam and {van Driel}, Roel",

year = "2008",

doi = "10.1007/s10577-008-1239-1",

language = "English",

volume = "16",

pages = "511--22",

journal = "Chromosome research : an international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology",

issn = "0967-3849",

publisher = "Springer Verlag",

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Intracellular manipulation of chromatin using magnetic nanoparticles. / Kanger, Johannes S; Subramaniam, Vinod; van Driel, Roel.
In: Chromosome research : an international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology, Vol. 16, No. 3, 2008, p. 511-22.

Research output: Contribution to Journal › Review article › Academic › peer-review

TY - JOUR

T1 - Intracellular manipulation of chromatin using magnetic nanoparticles

AU - Kanger, Johannes S

AU - Subramaniam, Vinod

AU - van Driel, Roel

PY - 2008

Y1 - 2008

N2 - Magnetic tweezers are widely used for manipulating small magnetic beads inside the cell cytoplasm in order to gain insight into the structural and mechanical properties of the cytoskeleton. Here we discuss the use of magnetic tweezers for the study of nuclear architecture and the mechanical properties of chromatin in living cells. A custom-built, dedicated micro magnetic tweezer set-up is described. We review progress that has been made in applying this technology for the study of chromatin structure and discuss its prospects for the in situ analysis of nuclear architecture and chromatin function.

AB - Magnetic tweezers are widely used for manipulating small magnetic beads inside the cell cytoplasm in order to gain insight into the structural and mechanical properties of the cytoskeleton. Here we discuss the use of magnetic tweezers for the study of nuclear architecture and the mechanical properties of chromatin in living cells. A custom-built, dedicated micro magnetic tweezer set-up is described. We review progress that has been made in applying this technology for the study of chromatin structure and discuss its prospects for the in situ analysis of nuclear architecture and chromatin function.

KW - Biomechanical Phenomena

KW - Chromatin

KW - HeLa Cells

KW - Humans

KW - Magnetics

KW - Metal Nanoparticles

KW - Microscopy, Atomic Force

KW - Models, Biological

KW - Nanotechnology

KW - Optical Tweezers

KW - Rheology

KW - Journal Article

KW - Review

U2 - 10.1007/s10577-008-1239-1

DO - 10.1007/s10577-008-1239-1

M3 - Review article

C2 - 18461487

SN - 0967-3849

VL - 16

SP - 511

EP - 522

JO - Chromosome research : an international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology

JF - Chromosome research : an international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology

IS - 3

ER -

Intracellular manipulation of chromatin using magnetic nanoparticles

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Keywords

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