Intracellular manipulation of chromatin using magnetic nanoparticles

Johannes S Kanger, Vinod Subramaniam, Roel van Driel

    Research output: Contribution to JournalReview articleAcademicpeer-review

    Abstract

    Magnetic tweezers are widely used for manipulating small magnetic beads inside the cell cytoplasm in order to gain insight into the structural and mechanical properties of the cytoskeleton. Here we discuss the use of magnetic tweezers for the study of nuclear architecture and the mechanical properties of chromatin in living cells. A custom-built, dedicated micro magnetic tweezer set-up is described. We review progress that has been made in applying this technology for the study of chromatin structure and discuss its prospects for the in situ analysis of nuclear architecture and chromatin function.

    Original languageEnglish
    Pages (from-to)511-22
    Number of pages12
    JournalChromosome research : an international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology
    Volume16
    Issue number3
    DOIs
    Publication statusPublished - 2008

    Keywords

    • Biomechanical Phenomena
    • Chromatin
    • HeLa Cells
    • Humans
    • Magnetics
    • Metal Nanoparticles
    • Microscopy, Atomic Force
    • Models, Biological
    • Nanotechnology
    • Optical Tweezers
    • Rheology
    • Journal Article
    • Review

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