TY - JOUR
T1 - Intraintestinal fermentation of fructo- and galacto-oligosaccharides and the fate of short-chain fatty acids in humans
AU - van Trijp, Mara P.H.
AU - Rios-Morales, Melany
AU - Witteman, Ben
AU - Abegaz, Fentaw
AU - Gerding, Albert
AU - An, Ran
AU - Koehorst, Martijn
AU - Evers, Bernard
AU - van Dongen, Katja C.V.
AU - Zoetendal, Erwin G.
AU - Schols, Henk
AU - Afman, Lydia A.
AU - Reijngoud, Dirk Jan
AU - Bakker, Barbara M.
AU - Hooiveld, Guido J.
N1 - Publisher Copyright:
© 2024 The Author(s)
PY - 2024/3/15
Y1 - 2024/3/15
N2 - Consumption of fructo- (FOS) and galacto-oligosaccharides (GOS) has health benefits which have been linked in part to short-chain fatty acids (SCFA) production by the gut microbiota. However, detailed knowledge of this process in the human intestine is lacking. We aimed to determine the acute fermentation kinetics of a FOS:GOS mixture in healthy males using a naso-intestinal catheter for sampling directly in the ileum or colon. We studied the fate of SCFA as substrates for glucose and lipid metabolism by the host after infusion of 13C-SCFA. In the human distal ileum, no fermentation of FOS:GOS, nor SCFA production, or bacterial cross-feeding was observed. The relative composition of intestinal microbiota changed rapidly during the test day, which demonstrates the relevance of postprandial intestinal sampling to track acute responses of the microbial community toward interventions. SCFA were vividly taken up and metabolized by the host as shown by incorporation of 13C in various host metabolites.
AB - Consumption of fructo- (FOS) and galacto-oligosaccharides (GOS) has health benefits which have been linked in part to short-chain fatty acids (SCFA) production by the gut microbiota. However, detailed knowledge of this process in the human intestine is lacking. We aimed to determine the acute fermentation kinetics of a FOS:GOS mixture in healthy males using a naso-intestinal catheter for sampling directly in the ileum or colon. We studied the fate of SCFA as substrates for glucose and lipid metabolism by the host after infusion of 13C-SCFA. In the human distal ileum, no fermentation of FOS:GOS, nor SCFA production, or bacterial cross-feeding was observed. The relative composition of intestinal microbiota changed rapidly during the test day, which demonstrates the relevance of postprandial intestinal sampling to track acute responses of the microbial community toward interventions. SCFA were vividly taken up and metabolized by the host as shown by incorporation of 13C in various host metabolites.
KW - Human metabolism
UR - http://www.scopus.com/inward/record.url?scp=85188218294&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85188218294&partnerID=8YFLogxK
U2 - 10.1016/j.isci.2024.109208
DO - 10.1016/j.isci.2024.109208
M3 - Article
AN - SCOPUS:85188218294
SN - 2589-0042
VL - 27
SP - 1
EP - 23
JO - iScience
JF - iScience
IS - 3
M1 - 109208
ER -