Intraintestinal fermentation of fructo- and galacto-oligosaccharides and the fate of short-chain fatty acids in humans

Mara P.H. van Trijp; Melany Rios-Morales; Ben Witteman; Fentaw Abegaz; Albert Gerding; Ran An; Martijn Koehorst; Bernard Evers; Katja C.V. van Dongen; Erwin G. Zoetendal; Henk Schols; Lydia A. Afman; Dirk Jan Reijngoud; Barbara M. Bakker; Guido J. Hooiveld

doi:10.1016/j.isci.2024.109208

Intraintestinal fermentation of fructo- and galacto-oligosaccharides and the fate of short-chain fatty acids in humans

Mara P.H. van Trijp, Melany Rios-Morales, Ben Witteman, Fentaw Abegaz, Albert Gerding, Ran An, Martijn Koehorst, Bernard Evers, Katja C.V. van Dongen, Erwin G. Zoetendal, Henk Schols, Lydia A. Afman, Dirk Jan Reijngoud, Barbara M. Bakker^*, Guido J. Hooiveld^*

^*Corresponding author for this work

Research output: Contribution to Journal › Article › Academic › peer-review

Abstract

Consumption of fructo- (FOS) and galacto-oligosaccharides (GOS) has health benefits which have been linked in part to short-chain fatty acids (SCFA) production by the gut microbiota. However, detailed knowledge of this process in the human intestine is lacking. We aimed to determine the acute fermentation kinetics of a FOS:GOS mixture in healthy males using a naso-intestinal catheter for sampling directly in the ileum or colon. We studied the fate of SCFA as substrates for glucose and lipid metabolism by the host after infusion of ¹³C-SCFA. In the human distal ileum, no fermentation of FOS:GOS, nor SCFA production, or bacterial cross-feeding was observed. The relative composition of intestinal microbiota changed rapidly during the test day, which demonstrates the relevance of postprandial intestinal sampling to track acute responses of the microbial community toward interventions. SCFA were vividly taken up and metabolized by the host as shown by incorporation of ¹³C in various host metabolites.

Original language	English
Article number	109208
Pages (from-to)	1-23
Number of pages	23
Journal	iScience
Volume	27
Issue number	3
Early online date	10 Feb 2024
DOIs	https://doi.org/10.1016/j.isci.2024.109208
Publication status	Published - 15 Mar 2024

Bibliographical note

Publisher Copyright:
© 2024 The Author(s)

Funding

Funders	Funder number
FrieslandCampina
Nutrition Sciences N.V.
Agrifirm Innovation Center B.V.
Universitair Medisch Centrum Groningen
DSM Food Specialties B.V.
Agrifirm Innovation Center B.V. Cooperatie
DSM Food Specialties B.V. FrieslandCampina Nederland B.V. Nutrition Sciences N.V. VanDrie Holding N.V.
KWF Kankerbestrijding	KWF 10913/2017-1
DSM Nutritional	645.001.001/3501
Not added	10913

Keywords

Human metabolism

Access to Document

10.1016/j.isci.2024.109208

Persistent URL (handle)

Persistent link

Cite this

van Trijp, M. P. H., Rios-Morales, M., Witteman, B., Abegaz, F., Gerding, A., An, R., Koehorst, M., Evers, B., van Dongen, K. C. V., Zoetendal, E. G., Schols, H., Afman, L. A., Reijngoud, D. J., Bakker, B. M., & Hooiveld, G. J. (2024). Intraintestinal fermentation of fructo- and galacto-oligosaccharides and the fate of short-chain fatty acids in humans. iScience, 27(3), 1-23. Article 109208. https://doi.org/10.1016/j.isci.2024.109208

@article{892856c089704ce7bbe0908c68bab099,

title = "Intraintestinal fermentation of fructo- and galacto-oligosaccharides and the fate of short-chain fatty acids in humans",

abstract = "Consumption of fructo- (FOS) and galacto-oligosaccharides (GOS) has health benefits which have been linked in part to short-chain fatty acids (SCFA) production by the gut microbiota. However, detailed knowledge of this process in the human intestine is lacking. We aimed to determine the acute fermentation kinetics of a FOS:GOS mixture in healthy males using a naso-intestinal catheter for sampling directly in the ileum or colon. We studied the fate of SCFA as substrates for glucose and lipid metabolism by the host after infusion of 13C-SCFA. In the human distal ileum, no fermentation of FOS:GOS, nor SCFA production, or bacterial cross-feeding was observed. The relative composition of intestinal microbiota changed rapidly during the test day, which demonstrates the relevance of postprandial intestinal sampling to track acute responses of the microbial community toward interventions. SCFA were vividly taken up and metabolized by the host as shown by incorporation of 13C in various host metabolites.",

keywords = "Human metabolism",

author = "\{van Trijp\}, \{Mara P.H.\} and Melany Rios-Morales and Ben Witteman and Fentaw Abegaz and Albert Gerding and Ran An and Martijn Koehorst and Bernard Evers and \{van Dongen\}, \{Katja C.V.\} and Zoetendal, \{Erwin G.\} and Henk Schols and Afman, \{Lydia A.\} and Reijngoud, \{Dirk Jan\} and Bakker, \{Barbara M.\} and Hooiveld, \{Guido J.\}",

note = "Publisher Copyright: {\textcopyright} 2024 The Author(s)",

year = "2024",

month = mar,

day = "15",

doi = "10.1016/j.isci.2024.109208",

language = "English",

volume = "27",

pages = "1--23",

journal = "iScience",

issn = "2589-0042",

publisher = "Elsevier Inc.",

number = "3",

}

van Trijp, MPH, Rios-Morales, M, Witteman, B, Abegaz, F, Gerding, A, An, R, Koehorst, M, Evers, B, van Dongen, KCV, Zoetendal, EG, Schols, H, Afman, LA, Reijngoud, DJ, Bakker, BM & Hooiveld, GJ 2024, 'Intraintestinal fermentation of fructo- and galacto-oligosaccharides and the fate of short-chain fatty acids in humans', iScience, vol. 27, no. 3, 109208, pp. 1-23. https://doi.org/10.1016/j.isci.2024.109208

TY - JOUR

T1 - Intraintestinal fermentation of fructo- and galacto-oligosaccharides and the fate of short-chain fatty acids in humans

AU - van Trijp, Mara P.H.

AU - Rios-Morales, Melany

AU - Witteman, Ben

AU - Abegaz, Fentaw

AU - Gerding, Albert

AU - An, Ran

AU - Koehorst, Martijn

AU - Evers, Bernard

AU - van Dongen, Katja C.V.

AU - Zoetendal, Erwin G.

AU - Schols, Henk

AU - Afman, Lydia A.

AU - Reijngoud, Dirk Jan

AU - Bakker, Barbara M.

AU - Hooiveld, Guido J.

PY - 2024/3/15

Y1 - 2024/3/15

N2 - Consumption of fructo- (FOS) and galacto-oligosaccharides (GOS) has health benefits which have been linked in part to short-chain fatty acids (SCFA) production by the gut microbiota. However, detailed knowledge of this process in the human intestine is lacking. We aimed to determine the acute fermentation kinetics of a FOS:GOS mixture in healthy males using a naso-intestinal catheter for sampling directly in the ileum or colon. We studied the fate of SCFA as substrates for glucose and lipid metabolism by the host after infusion of 13C-SCFA. In the human distal ileum, no fermentation of FOS:GOS, nor SCFA production, or bacterial cross-feeding was observed. The relative composition of intestinal microbiota changed rapidly during the test day, which demonstrates the relevance of postprandial intestinal sampling to track acute responses of the microbial community toward interventions. SCFA were vividly taken up and metabolized by the host as shown by incorporation of 13C in various host metabolites.

AB - Consumption of fructo- (FOS) and galacto-oligosaccharides (GOS) has health benefits which have been linked in part to short-chain fatty acids (SCFA) production by the gut microbiota. However, detailed knowledge of this process in the human intestine is lacking. We aimed to determine the acute fermentation kinetics of a FOS:GOS mixture in healthy males using a naso-intestinal catheter for sampling directly in the ileum or colon. We studied the fate of SCFA as substrates for glucose and lipid metabolism by the host after infusion of 13C-SCFA. In the human distal ileum, no fermentation of FOS:GOS, nor SCFA production, or bacterial cross-feeding was observed. The relative composition of intestinal microbiota changed rapidly during the test day, which demonstrates the relevance of postprandial intestinal sampling to track acute responses of the microbial community toward interventions. SCFA were vividly taken up and metabolized by the host as shown by incorporation of 13C in various host metabolites.

KW - Human metabolism

UR - https://www.scopus.com/pages/publications/85188218294

UR - https://www.scopus.com/inward/citedby.url?scp=85188218294&partnerID=8YFLogxK

U2 - 10.1016/j.isci.2024.109208

DO - 10.1016/j.isci.2024.109208

M3 - Article

AN - SCOPUS:85188218294

SN - 2589-0042

VL - 27

SP - 1

EP - 23

JO - iScience

JF - iScience

IS - 3

M1 - 109208

ER -

Intraintestinal fermentation of fructo- and galacto-oligosaccharides and the fate of short-chain fatty acids in humans

Abstract

Bibliographical note

Funding

Keywords

Access to Document

Persistent URL (handle)

Other files and links

Fingerprint

Cite this