TY - GEN
T1 - Iris yellow spot virus in the Netherlands
T2 - Occurrence in onion and confirmation of transmission by thrips tabaci
AU - Hoedjes, K.
AU - Verhoeven, J. T.J.
AU - Goldbach, R.
AU - Peters, D.
PY - 2011/7/31
Y1 - 2011/7/31
N2 - Since its first detection in the Netherlands in 1992 Iris yellow spot virus (IYSV, genus Tospovirus) has been reported worldwide in Allium crops, in a few ornamentals and in a small number of weeds. After recent findings of IYSV in Alstroemeria and Eustoma in the Netherlands, a number of neighbouring onion fields were surveyed. In 2005 and 2006, only few infected plants were found with obvious symptoms of IYSV. In 2007, after sampling and testing small leaf samples with various types of damage, including small brown-yellow colored spots and spots with thrips feeding damage, a high percentage of plants were found with positive IYSV scores in ELISA. Infection by IYSV could be confirmed in most ELISA-positive samples by RT-PCR. Under laboratory conditions, evidence was obtained that Thrips tabaci acts as a vector for this virus. Acquisition of the IYSV from infected Datura stramonium plants resulted in virus uptake and replication in over 60% of the thrips exposed, as determined by Western blotting and immunolocalisation of the virus in the foregut, and in epithelial and midgut muscle cells using antibodies against both the viral N and NSs proteins. Successful transmission of IYSV to seedlings of Emilia sonchifolia was observed, however, only at low frequency under the laboratory conditions used.
AB - Since its first detection in the Netherlands in 1992 Iris yellow spot virus (IYSV, genus Tospovirus) has been reported worldwide in Allium crops, in a few ornamentals and in a small number of weeds. After recent findings of IYSV in Alstroemeria and Eustoma in the Netherlands, a number of neighbouring onion fields were surveyed. In 2005 and 2006, only few infected plants were found with obvious symptoms of IYSV. In 2007, after sampling and testing small leaf samples with various types of damage, including small brown-yellow colored spots and spots with thrips feeding damage, a high percentage of plants were found with positive IYSV scores in ELISA. Infection by IYSV could be confirmed in most ELISA-positive samples by RT-PCR. Under laboratory conditions, evidence was obtained that Thrips tabaci acts as a vector for this virus. Acquisition of the IYSV from infected Datura stramonium plants resulted in virus uptake and replication in over 60% of the thrips exposed, as determined by Western blotting and immunolocalisation of the virus in the foregut, and in epithelial and midgut muscle cells using antibodies against both the viral N and NSs proteins. Successful transmission of IYSV to seedlings of Emilia sonchifolia was observed, however, only at low frequency under the laboratory conditions used.
KW - Immunodetection
KW - Onion thrips
KW - Tospovirus
KW - Whole mount immunofluorescent staining
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U2 - 10.17660/ActaHortic.2011.901.25
DO - 10.17660/ActaHortic.2011.901.25
M3 - Conference contribution
AN - SCOPUS:80053234327
SN - 9789066052536
T3 - Acta Horticulturae
SP - 199
EP - 206
BT - XII International Symposium on Virus Diseases of Ornamental Plants
PB - International Society for Horticultural Science
ER -