Isolation and characterization of gallium resistant Pseudomonas aeruginosa mutants

R García-Contreras, E Lira-Silva, R Jasso-Chávez, I.L. Hernández-González, T. Maeda, T. Hashimoto, F.C. Boogerd, L Sheng, TK Wood, R Moreno-Sánchez

Research output: Contribution to JournalArticleAcademicpeer-review

Abstract

Pseudomonas aeruginosa PA14 cells resistant to the novel antimicrobial gallium nitrate (Ga) were developed using transposon mutagenesis and by selecting spontaneous mutants. The mutants showing the highest growth in the presence of Ga were selected for further characterization. These mutants showed 4- to 12-fold higher Ga minimal inhibitory growth concentrations and a greater than 8-fold increase in the minimum biofilm eliminating Ga concentration. Both types of mutants produced Ga resistant biofilms whereas the formation of wild-type biofilms was strongly inhibited by Ga. The gene interrupted in the transposon mutant was hitA, which encodes a periplasmic iron binding protein that delivers Fe
Original languageEnglish
Pages (from-to)574-582
JournalInternational Journal of Medical Microbiology
Volume303
DOIs
Publication statusPublished - 2013

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