Isolation and characterization of gallium resistant Pseudomonas aeruginosa mutants

R García-Contreras; E Lira-Silva; R Jasso-Chávez; I.L. Hernández-González; T. Maeda; T. Hashimoto; F.C. Boogerd; L Sheng; TK Wood; R Moreno-Sánchez

doi:10.1016/j.ijmm.2013.07.009

Isolation and characterization of gallium resistant Pseudomonas aeruginosa mutants

R García-Contreras, E Lira-Silva, R Jasso-Chávez, I.L. Hernández-González, T. Maeda, T. Hashimoto, F.C. Boogerd, L Sheng, TK Wood, R Moreno-Sánchez

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Research output: Contribution to Journal › Article › Academic › peer-review

Abstract

Pseudomonas aeruginosa PA14 cells resistant to the novel antimicrobial gallium nitrate (Ga) were developed using transposon mutagenesis and by selecting spontaneous mutants. The mutants showing the highest growth in the presence of Ga were selected for further characterization. These mutants showed 4- to 12-fold higher Ga minimal inhibitory growth concentrations and a greater than 8-fold increase in the minimum biofilm eliminating Ga concentration. Both types of mutants produced Ga resistant biofilms whereas the formation of wild-type biofilms was strongly inhibited by Ga. The gene interrupted in the transposon mutant was hitA, which encodes a periplasmic iron binding protein that delivers Fe

Original language	English
Pages (from-to)	574-582
Journal	International Journal of Medical Microbiology
Volume	303
DOIs	https://doi.org/10.1016/j.ijmm.2013.07.009
Publication status	Published - 2013

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10.1016/j.ijmm.2013.07.009

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title = "Isolation and characterization of gallium resistant Pseudomonas aeruginosa mutants",

abstract = "Pseudomonas aeruginosa PA14 cells resistant to the novel antimicrobial gallium nitrate (Ga) were developed using transposon mutagenesis and by selecting spontaneous mutants. The mutants showing the highest growth in the presence of Ga were selected for further characterization. These mutants showed 4- to 12-fold higher Ga minimal inhibitory growth concentrations and a greater than 8-fold increase in the minimum biofilm eliminating Ga concentration. Both types of mutants produced Ga resistant biofilms whereas the formation of wild-type biofilms was strongly inhibited by Ga. The gene interrupted in the transposon mutant was hitA, which encodes a periplasmic iron binding protein that delivers Fe",

author = "R Garc{\'i}a-Contreras and E Lira-Silva and R Jasso-Ch{\'a}vez and I.L. Hern{\'a}ndez-Gonz{\'a}lez and T. Maeda and T. Hashimoto and F.C. Boogerd and L Sheng and TK Wood and R Moreno-S{\'a}nchez",

year = "2013",

doi = "10.1016/j.ijmm.2013.07.009",

language = "English",

volume = "303",

pages = "574--582",

journal = "International Journal of Medical Microbiology",

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TY - JOUR

T1 - Isolation and characterization of gallium resistant Pseudomonas aeruginosa mutants

AU - García-Contreras, R

AU - Lira-Silva, E

AU - Jasso-Chávez, R

AU - Hernández-González, I.L.

AU - Maeda, T.

AU - Hashimoto, T.

AU - Boogerd, F.C.

AU - Sheng, L

AU - Wood, TK

AU - Moreno-Sánchez, R

PY - 2013

Y1 - 2013

N2 - Pseudomonas aeruginosa PA14 cells resistant to the novel antimicrobial gallium nitrate (Ga) were developed using transposon mutagenesis and by selecting spontaneous mutants. The mutants showing the highest growth in the presence of Ga were selected for further characterization. These mutants showed 4- to 12-fold higher Ga minimal inhibitory growth concentrations and a greater than 8-fold increase in the minimum biofilm eliminating Ga concentration. Both types of mutants produced Ga resistant biofilms whereas the formation of wild-type biofilms was strongly inhibited by Ga. The gene interrupted in the transposon mutant was hitA, which encodes a periplasmic iron binding protein that delivers Fe

AB - Pseudomonas aeruginosa PA14 cells resistant to the novel antimicrobial gallium nitrate (Ga) were developed using transposon mutagenesis and by selecting spontaneous mutants. The mutants showing the highest growth in the presence of Ga were selected for further characterization. These mutants showed 4- to 12-fold higher Ga minimal inhibitory growth concentrations and a greater than 8-fold increase in the minimum biofilm eliminating Ga concentration. Both types of mutants produced Ga resistant biofilms whereas the formation of wild-type biofilms was strongly inhibited by Ga. The gene interrupted in the transposon mutant was hitA, which encodes a periplasmic iron binding protein that delivers Fe

UR - https://www.scopus.com/pages/publications/84887611892

UR - https://www.scopus.com/inward/citedby.url?scp=84887611892&partnerID=8YFLogxK

U2 - 10.1016/j.ijmm.2013.07.009

DO - 10.1016/j.ijmm.2013.07.009

M3 - Article

SN - 1438-4221

VL - 303

SP - 574

EP - 582

JO - International Journal of Medical Microbiology

JF - International Journal of Medical Microbiology

ER -

Isolation and characterization of gallium resistant Pseudomonas aeruginosa mutants

Abstract

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