Isolation of primary avian osteocytes

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Osteocytes can be isolated from chicken calvaria using mild EDTA treatment alternating with collagenase treatment. The cell population obtained contains both osteoblasts and osteocytes. A pure population of osteocytes is obtained following immunomagnetic separation with the osteocyte-specific monoclonal antibody MAb OB7.3.
Original languageEnglish
Pages (from-to)43-53
JournalMethods in Molecular Biology
Publication statusPublished - 2012


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