Isolation of primary avian osteocytes

C.M. Semeins; A.D. Bakker; J. Klein-Nulend

doi:10.1007/978-1-61779-415-5_4

Isolation of primary avian osteocytes

C.M. Semeins, A.D. Bakker, J. Klein-Nulend

Oral Cell Biology

Research output: Contribution to Journal › Article › Academic › peer-review

Abstract

Osteocytes can be isolated from chicken calvaria using mild EDTA treatment alternating with collagenase treatment. The cell population obtained contains both osteoblasts and osteocytes. A pure population of osteocytes is obtained following immunomagnetic separation with the osteocyte-specific monoclonal antibody MAb OB7.3.

Original language	English
Pages (from-to)	43-53
Journal	Methods in Molecular Biology
Volume	816
DOIs	https://doi.org/10.1007/978-1-61779-415-5_4
Publication status	Published - 2012

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

Access to Document

10.1007/978-1-61779-415-5_4

Handle.net

Persistent link

Cite this

@article{7463f205979144e592bc659a1e397cca,

title = "Isolation of primary avian osteocytes",

abstract = "Osteocytes can be isolated from chicken calvaria using mild EDTA treatment alternating with collagenase treatment. The cell population obtained contains both osteoblasts and osteocytes. A pure population of osteocytes is obtained following immunomagnetic separation with the osteocyte-specific monoclonal antibody MAb OB7.3.",

author = "C.M. Semeins and A.D. Bakker and J. Klein-Nulend",

year = "2012",

doi = "10.1007/978-1-61779-415-5_4",

language = "English",

volume = "816",

pages = "43--53",

journal = "Methods in molecular biology (Clifton, N.J.)",

issn = "1064-3745",

publisher = "Humana Press",

}

TY - JOUR

T1 - Isolation of primary avian osteocytes

AU - Semeins, C.M.

AU - Bakker, A.D.

AU - Klein-Nulend, J.

PY - 2012

Y1 - 2012

N2 - Osteocytes can be isolated from chicken calvaria using mild EDTA treatment alternating with collagenase treatment. The cell population obtained contains both osteoblasts and osteocytes. A pure population of osteocytes is obtained following immunomagnetic separation with the osteocyte-specific monoclonal antibody MAb OB7.3.

AB - Osteocytes can be isolated from chicken calvaria using mild EDTA treatment alternating with collagenase treatment. The cell population obtained contains both osteoblasts and osteocytes. A pure population of osteocytes is obtained following immunomagnetic separation with the osteocyte-specific monoclonal antibody MAb OB7.3.

U2 - 10.1007/978-1-61779-415-5_4

DO - 10.1007/978-1-61779-415-5_4

M3 - Article

SN - 1064-3745

VL - 816

SP - 43

EP - 53

JO - Methods in molecular biology (Clifton, N.J.)

JF - Methods in molecular biology (Clifton, N.J.)

ER -

Isolation of primary avian osteocytes

Abstract

UN SDGs

Access to Document

Handle.net

Fingerprint

Cite this