Isolation of primary avian osteocytes

C.M. Semeins; A.D. Bakker; J. Klein-Nulend

doi:10.1007/978-1-61779-415-5_4

Isolation of primary avian osteocytes

C.M. Semeins
, A.D. Bakker
, J. Klein-Nulend

Oral Cell Biology

Research output: Contribution to Journal › Article › Academic › peer-review

Abstract

Osteocytes can be isolated from chicken calvaria using mild EDTA treatment alternating with collagenase treatment. The cell population obtained contains both osteoblasts and osteocytes. A pure population of osteocytes is obtained following immunomagnetic separation with the osteocyte-specific monoclonal antibody MAb OB7.3.

Original language	English
Pages (from-to)	43-53
Journal	Methods in Molecular Biology
Volume	816
DOIs	https://doi.org/10.1007/978-1-61779-415-5_4
Publication status	Published - 2012

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SDG 3 Good Health and Well-being

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10.1007/978-1-61779-415-5_4

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title = "Isolation of primary avian osteocytes",

abstract = "Osteocytes can be isolated from chicken calvaria using mild EDTA treatment alternating with collagenase treatment. The cell population obtained contains both osteoblasts and osteocytes. A pure population of osteocytes is obtained following immunomagnetic separation with the osteocyte-specific monoclonal antibody MAb OB7.3.",

author = "C.M. Semeins and A.D. Bakker and J. Klein-Nulend",

year = "2012",

doi = "10.1007/978-1-61779-415-5\_4",

language = "English",

volume = "816",

pages = "43--53",

journal = "Methods in Molecular Biology",

issn = "1064-3745",

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T1 - Isolation of primary avian osteocytes

AU - Semeins, C.M.

AU - Bakker, A.D.

AU - Klein-Nulend, J.

PY - 2012

Y1 - 2012

N2 - Osteocytes can be isolated from chicken calvaria using mild EDTA treatment alternating with collagenase treatment. The cell population obtained contains both osteoblasts and osteocytes. A pure population of osteocytes is obtained following immunomagnetic separation with the osteocyte-specific monoclonal antibody MAb OB7.3.

AB - Osteocytes can be isolated from chicken calvaria using mild EDTA treatment alternating with collagenase treatment. The cell population obtained contains both osteoblasts and osteocytes. A pure population of osteocytes is obtained following immunomagnetic separation with the osteocyte-specific monoclonal antibody MAb OB7.3.

U2 - 10.1007/978-1-61779-415-5_4

DO - 10.1007/978-1-61779-415-5_4

M3 - Article

SN - 1064-3745

VL - 816

SP - 43

EP - 53

JO - Methods in Molecular Biology

JF - Methods in Molecular Biology

ER -

Isolation of primary avian osteocytes

Abstract

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