Isolation, sequencing, and mutagenesis of the gene encoding NAD- and glutathione-dependent formaldehyde dehydrogenase (GD-FALDH) from Paracoccus denitrificans, in which GD-FALDH is essential for methylotrophic growth

N.J.P. van Ras; P W Van Ophem; W N Reijnders; R J Van Spanning; J A Duine; A.H. Stouthamer; N Harms

Isolation, sequencing, and mutagenesis of the gene encoding NAD- and glutathione-dependent formaldehyde dehydrogenase (GD-FALDH) from Paracoccus denitrificans, in which GD-FALDH is essential for methylotrophic growth

N.J.P. van Ras, P W Van Ophem, W N Reijnders, R J Van Spanning, J A Duine, A.H. Stouthamer, N Harms

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Research output: Contribution to Journal › Article › Academic › peer-review

Abstract

NAD- and glutathione-dependent formaldehyde dehydrogenase (GD-FALDH) of Paracoccus denitrificans has been purified as a tetramer with a relative molecular mass of 150 kDa. The gene encoding GD-FALDH (flhA) has been isolated, sequenced, and mutated by insertion of a kanamycin resistance gene. The mutant strain is not able to grow on methanol, methylamine, or choline, while heterotrophic growth is not influenced by the mutation. This finding indicates that GD-FALDH of P. denitrificans is essential for the oxidation of formaldehyde produced during methylotrophic growth.

Original language	English
Pages (from-to)	247-51
Number of pages	5
Journal	Journal of Bacteriology
Volume	177
Issue number	1
Publication status	Published - Jan 1995

Keywords

Aldehyde Oxidoreductases
Amino Acid Sequence
Base Sequence
Cloning, Molecular
Formaldehyde
Genes, Bacterial
Methanol
Molecular Sequence Data
Mutagenesis
Paracoccus denitrificans
Sequence Analysis, DNA
Sequence Homology, Amino Acid
Comparative Study
Journal Article

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van Ras, N. J. P., Van Ophem, P. W., Reijnders, W. N., Van Spanning, R. J., Duine, J. A., Stouthamer, A. H., & Harms, N. (1995). Isolation, sequencing, and mutagenesis of the gene encoding NAD- and glutathione-dependent formaldehyde dehydrogenase (GD-FALDH) from Paracoccus denitrificans, in which GD-FALDH is essential for methylotrophic growth. Journal of Bacteriology, 177(1), 247-51.

@article{e1231ba6c3d44d11ade32f783348a954,

title = "Isolation, sequencing, and mutagenesis of the gene encoding NAD- and glutathione-dependent formaldehyde dehydrogenase (GD-FALDH) from Paracoccus denitrificans, in which GD-FALDH is essential for methylotrophic growth",

abstract = "NAD- and glutathione-dependent formaldehyde dehydrogenase (GD-FALDH) of Paracoccus denitrificans has been purified as a tetramer with a relative molecular mass of 150 kDa. The gene encoding GD-FALDH (flhA) has been isolated, sequenced, and mutated by insertion of a kanamycin resistance gene. The mutant strain is not able to grow on methanol, methylamine, or choline, while heterotrophic growth is not influenced by the mutation. This finding indicates that GD-FALDH of P. denitrificans is essential for the oxidation of formaldehyde produced during methylotrophic growth.",

keywords = "Aldehyde Oxidoreductases, Amino Acid Sequence, Base Sequence, Cloning, Molecular, Formaldehyde, Genes, Bacterial, Methanol, Molecular Sequence Data, Mutagenesis, Paracoccus denitrificans, Sequence Analysis, DNA, Sequence Homology, Amino Acid, Comparative Study, Journal Article",

author = "{van Ras}, N.J.P. and {Van Ophem}, {P W} and Reijnders, {W N} and {Van Spanning}, {R J} and Duine, {J A} and A.H. Stouthamer and N Harms",

year = "1995",

month = jan,

language = "English",

volume = "177",

pages = "247--51",

journal = "Journal of Bacteriology",

issn = "0021-9193",

publisher = "American Society for Microbiology",

number = "1",

}

van Ras, NJP, Van Ophem, PW, Reijnders, WN, Van Spanning, RJ, Duine, JA, Stouthamer, AH & Harms, N 1995, 'Isolation, sequencing, and mutagenesis of the gene encoding NAD- and glutathione-dependent formaldehyde dehydrogenase (GD-FALDH) from Paracoccus denitrificans, in which GD-FALDH is essential for methylotrophic growth', Journal of Bacteriology, vol. 177, no. 1, pp. 247-51.

Isolation, sequencing, and mutagenesis of the gene encoding NAD- and glutathione-dependent formaldehyde dehydrogenase (GD-FALDH) from Paracoccus denitrificans, in which GD-FALDH is essential for methylotrophic growth. / van Ras, N.J.P.; Van Ophem, P W; Reijnders, W N et al.
In: Journal of Bacteriology, Vol. 177, No. 1, 01.1995, p. 247-51.

Research output: Contribution to Journal › Article › Academic › peer-review

TY - JOUR

T1 - Isolation, sequencing, and mutagenesis of the gene encoding NAD- and glutathione-dependent formaldehyde dehydrogenase (GD-FALDH) from Paracoccus denitrificans, in which GD-FALDH is essential for methylotrophic growth

AU - van Ras, N.J.P.

AU - Van Ophem, P W

AU - Reijnders, W N

AU - Van Spanning, R J

AU - Duine, J A

AU - Stouthamer, A.H.

AU - Harms, N

PY - 1995/1

Y1 - 1995/1

N2 - NAD- and glutathione-dependent formaldehyde dehydrogenase (GD-FALDH) of Paracoccus denitrificans has been purified as a tetramer with a relative molecular mass of 150 kDa. The gene encoding GD-FALDH (flhA) has been isolated, sequenced, and mutated by insertion of a kanamycin resistance gene. The mutant strain is not able to grow on methanol, methylamine, or choline, while heterotrophic growth is not influenced by the mutation. This finding indicates that GD-FALDH of P. denitrificans is essential for the oxidation of formaldehyde produced during methylotrophic growth.

AB - NAD- and glutathione-dependent formaldehyde dehydrogenase (GD-FALDH) of Paracoccus denitrificans has been purified as a tetramer with a relative molecular mass of 150 kDa. The gene encoding GD-FALDH (flhA) has been isolated, sequenced, and mutated by insertion of a kanamycin resistance gene. The mutant strain is not able to grow on methanol, methylamine, or choline, while heterotrophic growth is not influenced by the mutation. This finding indicates that GD-FALDH of P. denitrificans is essential for the oxidation of formaldehyde produced during methylotrophic growth.

KW - Aldehyde Oxidoreductases

KW - Amino Acid Sequence

KW - Base Sequence

KW - Cloning, Molecular

KW - Formaldehyde

KW - Genes, Bacterial

KW - Methanol

KW - Molecular Sequence Data

KW - Mutagenesis

KW - Paracoccus denitrificans

KW - Sequence Analysis, DNA

KW - Sequence Homology, Amino Acid

KW - Comparative Study

KW - Journal Article

M3 - Article

C2 - 7798140

SN - 0021-9193

VL - 177

SP - 247

EP - 251

JO - Journal of Bacteriology

JF - Journal of Bacteriology

IS - 1

ER -