Knowledge on DNA success rates to optimize the DNA analysis process: from crime scene to laboratory

Anna A.  Mapes; Ate D. Kloosterman; Vincent van Marion; Christianne de Poot

doi:10.1111/1556-4029.13102

Knowledge on DNA success rates to optimize the DNA analysis process: from crime scene to laboratory

Anna A. Mapes, Ate D. Kloosterman, Vincent van Marion, Christianne de Poot

Research output: Contribution to Journal › Article › Academic › peer-review

Abstract

DNA analysis has become an essential intelligence tool in the criminal justice system for the identification of possible offenders. However, it appears that about half of the processed DNA samples contains too little DNA for analysis. This study looks at DNA success rates within 28 different categories of trace exhibits and relates the DNA concentration to the characteristics of the DNA profile. Data from 2260 analyzed crime samples show that cigarettes, bloodstains, and headwear have relatively high success rates. Cartridge cases, crowbars, and tie-wraps are on the other end of the spectrum. These objective data can assist forensics in their selection process.The DNA success probability shows a positive relation with the DNA concentration. This finding enables the laboratory to set an evidence-based threshold value in the DNA analysis process. For instance, 958 DNA extracts had a concentration value of 6 pg/μL or less. Only 46 of the 958 low-level extracts provided meaningful DNA profiling data.

Original language	English
Pages (from-to)	1055
Number of pages	1061
Journal	Journal of Forensic Sciences
Volume	61
Issue number	4
DOIs	https://doi.org/10.1111/1556-4029.13102
Publication status	Published - 7 Apr 2016
Externally published	Yes

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title = "Knowledge on DNA success rates to optimize the DNA analysis process: from crime scene to laboratory",

abstract = "DNA analysis has become an essential intelligence tool in the criminal justice system for the identification of possible offenders. However, it appears that about half of the processed DNA samples contains too little DNA for analysis. This study looks at DNA success rates within 28 different categories of trace exhibits and relates the DNA concentration to the characteristics of the DNA profile. Data from 2260 analyzed crime samples show that cigarettes, bloodstains, and headwear have relatively high success rates. Cartridge cases, crowbars, and tie-wraps are on the other end of the spectrum. These objective data can assist forensics in their selection process.The DNA success probability shows a positive relation with the DNA concentration. This finding enables the laboratory to set an evidence-based threshold value in the DNA analysis process. For instance, 958 DNA extracts had a concentration value of 6 pg/μL or less. Only 46 of the 958 low-level extracts provided meaningful DNA profiling data.",

author = "Mapes, {Anna A.} and Kloosterman, {Ate D.} and {van Marion}, Vincent and {de Poot}, Christianne",

year = "2016",

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T1 - Knowledge on DNA success rates to optimize the DNA analysis process: from crime scene to laboratory

AU - Mapes, Anna A.

AU - Kloosterman, Ate D.

AU - van Marion, Vincent

AU - de Poot, Christianne

PY - 2016/4/7

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N2 - DNA analysis has become an essential intelligence tool in the criminal justice system for the identification of possible offenders. However, it appears that about half of the processed DNA samples contains too little DNA for analysis. This study looks at DNA success rates within 28 different categories of trace exhibits and relates the DNA concentration to the characteristics of the DNA profile. Data from 2260 analyzed crime samples show that cigarettes, bloodstains, and headwear have relatively high success rates. Cartridge cases, crowbars, and tie-wraps are on the other end of the spectrum. These objective data can assist forensics in their selection process.The DNA success probability shows a positive relation with the DNA concentration. This finding enables the laboratory to set an evidence-based threshold value in the DNA analysis process. For instance, 958 DNA extracts had a concentration value of 6 pg/μL or less. Only 46 of the 958 low-level extracts provided meaningful DNA profiling data.

AB - DNA analysis has become an essential intelligence tool in the criminal justice system for the identification of possible offenders. However, it appears that about half of the processed DNA samples contains too little DNA for analysis. This study looks at DNA success rates within 28 different categories of trace exhibits and relates the DNA concentration to the characteristics of the DNA profile. Data from 2260 analyzed crime samples show that cigarettes, bloodstains, and headwear have relatively high success rates. Cartridge cases, crowbars, and tie-wraps are on the other end of the spectrum. These objective data can assist forensics in their selection process.The DNA success probability shows a positive relation with the DNA concentration. This finding enables the laboratory to set an evidence-based threshold value in the DNA analysis process. For instance, 958 DNA extracts had a concentration value of 6 pg/μL or less. Only 46 of the 958 low-level extracts provided meaningful DNA profiling data.

U2 - 10.1111/1556-4029.13102

DO - 10.1111/1556-4029.13102

M3 - Article

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JF - Journal of Forensic Sciences

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Knowledge on DNA success rates to optimize the DNA analysis process: from crime scene to laboratory

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