Label-Free Analysis with Multiple Parameters Separates G Protein-Coupled Receptor Signaling Pathways

Teemu Suutari*, Sabrina N. Rahman, Henry F. Vischer, Dick Van Iperen, Arto Merivaara, Marjo Yliperttula, Rob Leurs, Jeroen Kool, Tapani Viitala

*Corresponding author for this work

Research output: Contribution to JournalArticleAcademicpeer-review

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Real-time label-free techniques are used to profile G protein-coupled receptor (GPCR) signaling pathways in living cells. However, interpreting the label-free signal responses is challenging, and previously reported methods do not reliably separate pathways from each other. In this study, a continuous angular-scanning surface plasmon resonance (SPR) technique is utilized for measuring label-free GPCR signal profiles. We show how the continuous angular-scanning ability, measuring up to nine real-time label-free parameters simultaneously, results in more information-rich label-free signal profiles for different GPCR pathways, providing a more accurate pathway separation. For this, we measured real-time full-angular SPR response curves for Gs, Gq, and Gi signaling pathways in living cells. By selecting two of the most prominent label-free parameters: the full SPR curve angular and intensity shifts, we present how this analysis approach can separate each of the three signaling pathways in a straightforward single-step analysis setup, without concurrent use of signal inhibitors or other response modulating compounds.

Original languageEnglish
Pages (from-to)14509-14516
Number of pages8
JournalAnalytical chemistry
Issue number21
Early online date15 Oct 2020
Publication statusPublished - 3 Nov 2020


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