TY - JOUR
T1 - Label-free quantitative protein profiling of vastus lateralis muscle during human aging
AU - Théron, Laëtitia
AU - Gueugneau, Marine
AU - Coudy, Cécile
AU - Viala, Didier
AU - Bijlsma, Astrid
AU - Butler-Browne, Gillian
AU - Maier, Andrea
AU - Béchet, Daniel
AU - Chambon, Christophe
PY - 2014/1
Y1 - 2014/1
N2 - Sarcopenia corresponds to the loss of muscle mass occurring during aging, and is associated with a loss of muscle functionality. Proteomic links the muscle functional changes with protein expression pattern. To better understand the mechanisms involved in muscle aging, we performed a proteomic analysis of Vastus lateralis muscle in mature and older women. For this, a shotgun proteomic method was applied to identify soluble proteins in muscle, using a combination of high performance liquid chromatography and mass spectrometry. A label-free protein profiling was then conducted to quantify proteins and compare profiles from mature and older women. This analysis showed that 35 of the 366 identified proteins were linked to aging in muscle. Most of the proteins were under-represented in older compared with mature women. We built a functional interaction network linking the proteins differentially expressed between mature and older women. The results revealed that the main differences between mature and older women were defined by proteins involved in energy metabolism and proteins from the myofilament and cytoskeleton. This is the first time that label-free quantitative proteomics has been applied to study of aging mechanisms in human skeletal muscle. This approach highlights new elements for elucidating the alterations observed during aging and may lead to novel sarcopenia biomarkers. Molecular & Cellular Proteomics 13: 10.1074/mcp.M113.032698, 283-294, 2014. © 2014 by The American Society for Biochemistry and Molecular Biology, Inc.
AB - Sarcopenia corresponds to the loss of muscle mass occurring during aging, and is associated with a loss of muscle functionality. Proteomic links the muscle functional changes with protein expression pattern. To better understand the mechanisms involved in muscle aging, we performed a proteomic analysis of Vastus lateralis muscle in mature and older women. For this, a shotgun proteomic method was applied to identify soluble proteins in muscle, using a combination of high performance liquid chromatography and mass spectrometry. A label-free protein profiling was then conducted to quantify proteins and compare profiles from mature and older women. This analysis showed that 35 of the 366 identified proteins were linked to aging in muscle. Most of the proteins were under-represented in older compared with mature women. We built a functional interaction network linking the proteins differentially expressed between mature and older women. The results revealed that the main differences between mature and older women were defined by proteins involved in energy metabolism and proteins from the myofilament and cytoskeleton. This is the first time that label-free quantitative proteomics has been applied to study of aging mechanisms in human skeletal muscle. This approach highlights new elements for elucidating the alterations observed during aging and may lead to novel sarcopenia biomarkers. Molecular & Cellular Proteomics 13: 10.1074/mcp.M113.032698, 283-294, 2014. © 2014 by The American Society for Biochemistry and Molecular Biology, Inc.
UR - http://www.scopus.com/inward/record.url?scp=84891759563&partnerID=8YFLogxK
U2 - 10.1074/mcp.M113.032698
DO - 10.1074/mcp.M113.032698
M3 - Article
SN - 1535-9476
VL - 13
SP - 283
EP - 294
JO - Molecular and Cellular Proteomics
JF - Molecular and Cellular Proteomics
IS - 1
ER -