Large-scale automated histology in the pursuit of Connectomes

David Kleinfeld; Arjun Bharioke; Pablo Blinder; Davi D. Bock; Kevin L. Briggman; Dmitri B. Chklovskii; Winfried Denk; Moritz Helmstaedter; John P. Kaufhold; Wei-Chung Allen Lee; Hanno S. Meyer; Kristina D. Micheva; Marcel Oberlaender; Steffen Prohaska; R. Clay Reid; Stephen J. Smith; Shinya Takemura; Philbert S. Tsai; Bert Sakmann

doi:10.1523/JNEUROSCI.4077-11.2011

Large-scale automated histology in the pursuit of Connectomes

David Kleinfeld, Arjun Bharioke, Pablo Blinder, Davi D. Bock, Kevin L. Briggman, Dmitri B. Chklovskii, Winfried Denk, Moritz Helmstaedter, John P. Kaufhold, Wei-Chung Allen Lee, Hanno S. Meyer, Kristina D. Micheva, Marcel Oberlaender, Steffen Prohaska, R. Clay Reid, Stephen J. Smith, Shinya Takemura, Philbert S. Tsai, Bert Sakmann

Research output: Contribution to Journal › Article › Academic › peer-review

Abstract

How does the brain compute? Answering this question necessitates neuronal connectomes, annotated graphs of all synaptic connections within defined brain areas. Further, understanding the energetics of the brain's computations requires vascular graphs. The assembly of a connectome requires sensitive hardware tools to measure neuronal and neurovascular features in all three dimensions, as well as software and machine learning for data analysis and visualization. We present the state of the art on the reconstruction of circuits and vasculature that link brain anatomy and function. Analysis at the scale of tens of nanometers yields connections between identified neurons, while analysis at the micrometer scale yields probabilistic rules of connection between neurons and exact vascular connectivity. © 2011 the authors.

Original language	English
Pages (from-to)	16125-16138
Journal	Journal of Neuroscience
Volume	31
Issue number	45
DOIs	https://doi.org/10.1523/JNEUROSCI.4077-11.2011
Publication status	Published - 9 Nov 2011
Externally published	Yes

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Kleinfeld, D., Bharioke, A., Blinder, P., Bock, D. D., Briggman, K. L., Chklovskii, D. B., Denk, W., Helmstaedter, M., Kaufhold, J. P., Lee, W.-C. A., Meyer, H. S., Micheva, K. D., Oberlaender, M., Prohaska, S., Reid, R. C., Smith, S. J., Takemura, S., Tsai, P. S., & Sakmann, B. (2011). Large-scale automated histology in the pursuit of Connectomes. Journal of Neuroscience, 31(45), 16125-16138. https://doi.org/10.1523/JNEUROSCI.4077-11.2011

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title = "Large-scale automated histology in the pursuit of Connectomes",

abstract = "How does the brain compute? Answering this question necessitates neuronal connectomes, annotated graphs of all synaptic connections within defined brain areas. Further, understanding the energetics of the brain's computations requires vascular graphs. The assembly of a connectome requires sensitive hardware tools to measure neuronal and neurovascular features in all three dimensions, as well as software and machine learning for data analysis and visualization. We present the state of the art on the reconstruction of circuits and vasculature that link brain anatomy and function. Analysis at the scale of tens of nanometers yields connections between identified neurons, while analysis at the micrometer scale yields probabilistic rules of connection between neurons and exact vascular connectivity. {\textcopyright} 2011 the authors.",

author = "David Kleinfeld and Arjun Bharioke and Pablo Blinder and Bock, \{Davi D.\} and Briggman, \{Kevin L.\} and Chklovskii, \{Dmitri B.\} and Winfried Denk and Moritz Helmstaedter and Kaufhold, \{John P.\} and Lee, \{Wei-Chung Allen\} and Meyer, \{Hanno S.\} and Micheva, \{Kristina D.\} and Marcel Oberlaender and Steffen Prohaska and Reid, \{R. Clay\} and Smith, \{Stephen J.\} and Shinya Takemura and Tsai, \{Philbert S.\} and Bert Sakmann",

year = "2011",

month = nov,

day = "9",

doi = "10.1523/JNEUROSCI.4077-11.2011",

language = "English",

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Kleinfeld, D, Bharioke, A, Blinder, P, Bock, DD, Briggman, KL, Chklovskii, DB, Denk, W, Helmstaedter, M, Kaufhold, JP, Lee, W-CA, Meyer, HS, Micheva, KD, Oberlaender, M, Prohaska, S, Reid, RC, Smith, SJ, Takemura, S, Tsai, PS & Sakmann, B 2011, 'Large-scale automated histology in the pursuit of Connectomes', Journal of Neuroscience, vol. 31, no. 45, pp. 16125-16138. https://doi.org/10.1523/JNEUROSCI.4077-11.2011

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AU - Chklovskii, Dmitri B.

AU - Denk, Winfried

AU - Helmstaedter, Moritz

AU - Kaufhold, John P.

AU - Lee, Wei-Chung Allen

AU - Meyer, Hanno S.

AU - Micheva, Kristina D.

AU - Oberlaender, Marcel

AU - Prohaska, Steffen

AU - Reid, R. Clay

AU - Smith, Stephen J.

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AU - Sakmann, Bert

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Large-scale automated histology in the pursuit of Connectomes

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