Abstract
© 2019 Samantha HughesCaenorhabditis elegans is an invertebrate model organism used in many areas of biology including developmental biology and the identification of molecular mechanisms and pathways. However, several experimental approaches require large quantities of worms, which is limiting and time-consuming. We present a protocol that uses modern fermentation methodology to effectively produce large numbers of C. elegans using a 7-l bioreactor in a fed-batch cultivation procedure. The production is modular and flexible as well as being a self-controlled system, thus not much labor is required until harvesting C. elegans. The high-yield worm cultivation is flexible and simple to amend, and now allows for the extended application of C. elegans as a model organism and expression system, including large-scale protein production.
| Original language | English |
|---|---|
| Pages (from-to) | 33-39 |
| Journal | BioTechniques |
| Volume | 67 |
| Issue number | 1 |
| DOIs | |
| Publication status | Published - 2019 |
| Externally published | Yes |
Funding
This work was supported by the RAAK-Elegant! Project, funded by SiA 2014-01-07PRO and the German Research The authors would like to thank Nil van Hal (Han University of Applied Sciences, The Netherlands) for his help with the bacterial cultivation. Preliminary work was supported by Antje Müller (LeipzigUniversity,Germany), Eric van de Zilver and Rick van de Vonder-voort (both at HAN University of Applied Sciences, The Netherlands). Caenorhabditis elegans and bacterial strains were provided by the CGC, which is funded by ?IH O ?ce of Research Infrastructure Programs (P40 OD010440).
| Funders | Funder number |
|---|---|
| Antje Müller | |
| Eric van de Zilver | |
| HAN University of Applied Sciences | |
| Rick van de Vonder-voort | |
| NIH Office of the Director | P40OD010440 |
| Shenyang Institute of Automation | 2014-01-07PRO |