Large-scale overproduction, functional purification and ligand affinities of the His-tagged human histamine H1 receptor

V.R. Ratnala; H.J. Swarts; J. VanOostrum; R. Leurs; H.J. DeGroot; R.A. Bakker; W.J. DeGrip

doi:10.1111/j.1432-1033.2004.04192.x

Large-scale overproduction, functional purification and ligand affinities of the His-tagged human histamine H1 receptor

V.R. Ratnala, H.J. Swarts, J. VanOostrum, R. Leurs, H.J. DeGroot, R.A. Bakker, W.J. DeGrip

Medicinal chemistry

Research output: Contribution to Journal › Article › Academic › peer-review

Abstract

This report describes an efficient strategy for amplified functional purification of the human H1 receptor after heterologous expression in Sf9 cells. The cDNA encoding a C-terminally histidine-tagged (10xHis) human histamine H1 receptor was used to generate recombinant baculovirus in a Spodoptera frugiperda-derived cell line (IPLB-Sf9). As judged from its ligand affinity profile, functional receptor could be expressed at high levels (30-40 pmol per 10

Original language	English
Pages (from-to)	2636-46
Journal	European Journal of Biochemistry
Volume	271
Issue number	13
DOIs	https://doi.org/10.1111/j.1432-1033.2004.04192.x
Publication status	Published - 2004

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abstract = "This report describes an efficient strategy for amplified functional purification of the human H1 receptor after heterologous expression in Sf9 cells. The cDNA encoding a C-terminally histidine-tagged (10xHis) human histamine H1 receptor was used to generate recombinant baculovirus in a Spodoptera frugiperda-derived cell line (IPLB-Sf9). As judged from its ligand affinity profile, functional receptor could be expressed at high levels (30-40 pmol per 10",

author = "V.R. Ratnala and H.J. Swarts and J. VanOostrum and R. Leurs and H.J. DeGroot and R.A. Bakker and W.J. DeGrip",

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AU - Ratnala, V.R.

AU - Swarts, H.J.

AU - VanOostrum, J.

AU - Leurs, R.

AU - DeGroot, H.J.

AU - Bakker, R.A.

AU - DeGrip, W.J.

PY - 2004

Y1 - 2004

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AB - This report describes an efficient strategy for amplified functional purification of the human H1 receptor after heterologous expression in Sf9 cells. The cDNA encoding a C-terminally histidine-tagged (10xHis) human histamine H1 receptor was used to generate recombinant baculovirus in a Spodoptera frugiperda-derived cell line (IPLB-Sf9). As judged from its ligand affinity profile, functional receptor could be expressed at high levels (30-40 pmol per 10

U2 - 10.1111/j.1432-1033.2004.04192.x

DO - 10.1111/j.1432-1033.2004.04192.x

M3 - Article

SN - 0014-2956

VL - 271

SP - 2636

EP - 2646

JO - European Journal of Biochemistry

JF - European Journal of Biochemistry

IS - 13

ER -

Large-scale overproduction, functional purification and ligand affinities of the His-tagged human histamine H1 receptor

Abstract

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