Last in, first out: the role of cofactor binding the flavodoxin folding.

Y.J.M. Bollen, S.M. Nabuurs, W.J.H. Berkel, C.P.M. van Mierlo

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Abstract

Although many proteins require the binding of a ligand to be functional, the role of ligand binding during folding is scarcely investigated. Here, we have reported the influence of the flavin mononucleotide (FMN) cofactor on the global stability and folding kinetics of Azotobacter vinelandii holoflavodoxin. Earlier studies have revealed that A. vinelandii apoflavodoxin kinetically folds according to the four-state mechanism: I
Original languageEnglish
Pages (from-to)7836-7844
JournalJournal of Biological Chemistry
Volume280
DOIs
Publication statusPublished - 2005

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