@inproceedings{0d19c3c9e45847488913a8212723d956,
title = "Lattice light sheet microscopy and photo-stimulation in brain slices",
abstract = "Lattice light sheet (LLS) fluorescence microscopy is a powerful recent technique for in vivo imaging of single and multicellular samples at very high spatiooral resolutions. We built a LLS microscope in which we added a photostimulation path to perform all-optical neurophysiological studies in rodent hippocampal brain slices. Thanks to the photo-stimulation path we could achieve fluorescence recovery after photobleaching (FRAP) or glutamate uncaging at spatially and temporally controlled regions of interest. Several fluorescence labelling protocols were employed depending on the imaged structure. Sub-micrometric neuronal elements such as spines or dendritic vesicles could be imaged down to ∼20 μm below the surface. We demonstrate the performances of LLS in several ongoing studies: measurement of AMPA receptor surface diffusion at single spines, vesicular transport in dendrites, spontaneous and stimulated local calcium activity in neurons and astrocytes.",
author = "Mathieu Ducros and Angela Getz and Misa Arizono and Valeria Pecoraro and Monica Fernandez-Monreal and Mathieu Letellier and N{\"a}gerl, \{U. Valentin\} and Daniel Choquet",
year = "2019",
doi = "10.1117/12.2509467",
language = "English",
series = "Progress in Biomedical Optics and Imaging - Proceedings of SPIE",
publisher = "SPIE",
editor = "L. Fu and Q. Luo and J. Ding",
booktitle = "Neural Imaging and Sensing 2019",
note = "Neural Imaging and Sensing 2019 ; Conference date: 04-02-2019 Through 05-02-2019",
}