Linking cytochrome P450 enzymes from Mycobacterium tuberculosis to their cognate ferredoxin partners

S. Ortega Ugalde, Coen P de Koning, K. Wallraven, Ben Bruyneel, N.P.E. Vermeulen, T.N. Grossmann, W. Bitter, J.N.M. Commandeur, J.C. Vos

Research output: Contribution to JournalArticleAcademicpeer-review

Abstract

Mycobacterium tuberculosis (Mtb) codes for 20 cytochrome P450 enzymes (CYPs), considered potential drug-targets due to their essential roles in bacterial viability and host infection. Catalytic activity of mycobacterial CYPs is dependent on electron transfer from a NAD (P)H-ferredoxin-reductase (FNR) and a ferredoxin (Fd). Two FNRs (FdrA and FprA) and five ferredoxins (Fdx, FdxA, FdxC, FdxD, and Rv1786) have been found in the Mtb genome. However, as of yet, the cognate redox partnerships have not been fully established. This is confounded by the fact that heterologous redox partners are routinely used to reconstitute Mtb CYP metabolism. To this end, this study aimed to biochemically characterize and identify cognate redox partnerships for Mtb CYPs. Interestingly, all combinations of FNRs and ferredoxins were active in the reduction of oxidized cytochrome c, but steady-state kinetic assays revealed FdxD as the most efficient redox partner for FdrA, whereas Fdx coupled preferably with FprA. CYP121A1, CYP124A1, CYP125A1, and CYP142A1 metabolism with the cognate redox partners was reconstituted in vitro showing an unanticipated selectivity in the requirement for electron transfer partnership, which did not necessarily correlate with proximity in the genome. This is the first description of microbial P450 metabolism in which multiple ferredoxins are functionally linked to multiple CYPs.

Original languageEnglish
Pages (from-to)9231-9242
Number of pages12
JournalApplied Microbiology and Biotechnology
Volume102
Issue number21
Early online date22 Aug 2018
DOIs
Publication statusPublished - Nov 2018

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Ferredoxins
Mycobacterium tuberculosis
Cytochrome P-450 Enzyme System
Oxidation-Reduction
Genome
Electrons
Microbial Viability
Cytochromes c
NAD
Oxidoreductases
Infection
Pharmaceutical Preparations

Keywords

  • Cytochrome P450
  • Ferredoxin
  • Mycobacterium tuberculosis
  • NAD (P) H ferredoxin reductase
  • Redox partners

Cite this

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title = "Linking cytochrome P450 enzymes from Mycobacterium tuberculosis to their cognate ferredoxin partners",
abstract = "Mycobacterium tuberculosis (Mtb) codes for 20 cytochrome P450 enzymes (CYPs), considered potential drug-targets due to their essential roles in bacterial viability and host infection. Catalytic activity of mycobacterial CYPs is dependent on electron transfer from a NAD (P)H-ferredoxin-reductase (FNR) and a ferredoxin (Fd). Two FNRs (FdrA and FprA) and five ferredoxins (Fdx, FdxA, FdxC, FdxD, and Rv1786) have been found in the Mtb genome. However, as of yet, the cognate redox partnerships have not been fully established. This is confounded by the fact that heterologous redox partners are routinely used to reconstitute Mtb CYP metabolism. To this end, this study aimed to biochemically characterize and identify cognate redox partnerships for Mtb CYPs. Interestingly, all combinations of FNRs and ferredoxins were active in the reduction of oxidized cytochrome c, but steady-state kinetic assays revealed FdxD as the most efficient redox partner for FdrA, whereas Fdx coupled preferably with FprA. CYP121A1, CYP124A1, CYP125A1, and CYP142A1 metabolism with the cognate redox partners was reconstituted in vitro showing an unanticipated selectivity in the requirement for electron transfer partnership, which did not necessarily correlate with proximity in the genome. This is the first description of microbial P450 metabolism in which multiple ferredoxins are functionally linked to multiple CYPs.",
keywords = "Cytochrome P450, Ferredoxin, Mycobacterium tuberculosis, NAD (P) H ferredoxin reductase, Redox partners",
author = "{Ortega Ugalde}, S. and {de Koning}, {Coen P} and K. Wallraven and Ben Bruyneel and N.P.E. Vermeulen and T.N. Grossmann and W. Bitter and J.N.M. Commandeur and J.C. Vos",
year = "2018",
month = "11",
doi = "10.1007/s00253-018-9299-4",
language = "English",
volume = "102",
pages = "9231--9242",
journal = "Applied Microbiology and Biotechnology",
issn = "0175-7598",
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Linking cytochrome P450 enzymes from Mycobacterium tuberculosis to their cognate ferredoxin partners. / Ortega Ugalde, S.; de Koning, Coen P; Wallraven, K.; Bruyneel, Ben; Vermeulen, N.P.E.; Grossmann, T.N.; Bitter, W.; Commandeur, J.N.M.; Vos, J.C.

In: Applied Microbiology and Biotechnology, Vol. 102, No. 21, 11.2018, p. 9231-9242.

Research output: Contribution to JournalArticleAcademicpeer-review

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T1 - Linking cytochrome P450 enzymes from Mycobacterium tuberculosis to their cognate ferredoxin partners

AU - Ortega Ugalde, S.

AU - de Koning, Coen P

AU - Wallraven, K.

AU - Bruyneel, Ben

AU - Vermeulen, N.P.E.

AU - Grossmann, T.N.

AU - Bitter, W.

AU - Commandeur, J.N.M.

AU - Vos, J.C.

PY - 2018/11

Y1 - 2018/11

N2 - Mycobacterium tuberculosis (Mtb) codes for 20 cytochrome P450 enzymes (CYPs), considered potential drug-targets due to their essential roles in bacterial viability and host infection. Catalytic activity of mycobacterial CYPs is dependent on electron transfer from a NAD (P)H-ferredoxin-reductase (FNR) and a ferredoxin (Fd). Two FNRs (FdrA and FprA) and five ferredoxins (Fdx, FdxA, FdxC, FdxD, and Rv1786) have been found in the Mtb genome. However, as of yet, the cognate redox partnerships have not been fully established. This is confounded by the fact that heterologous redox partners are routinely used to reconstitute Mtb CYP metabolism. To this end, this study aimed to biochemically characterize and identify cognate redox partnerships for Mtb CYPs. Interestingly, all combinations of FNRs and ferredoxins were active in the reduction of oxidized cytochrome c, but steady-state kinetic assays revealed FdxD as the most efficient redox partner for FdrA, whereas Fdx coupled preferably with FprA. CYP121A1, CYP124A1, CYP125A1, and CYP142A1 metabolism with the cognate redox partners was reconstituted in vitro showing an unanticipated selectivity in the requirement for electron transfer partnership, which did not necessarily correlate with proximity in the genome. This is the first description of microbial P450 metabolism in which multiple ferredoxins are functionally linked to multiple CYPs.

AB - Mycobacterium tuberculosis (Mtb) codes for 20 cytochrome P450 enzymes (CYPs), considered potential drug-targets due to their essential roles in bacterial viability and host infection. Catalytic activity of mycobacterial CYPs is dependent on electron transfer from a NAD (P)H-ferredoxin-reductase (FNR) and a ferredoxin (Fd). Two FNRs (FdrA and FprA) and five ferredoxins (Fdx, FdxA, FdxC, FdxD, and Rv1786) have been found in the Mtb genome. However, as of yet, the cognate redox partnerships have not been fully established. This is confounded by the fact that heterologous redox partners are routinely used to reconstitute Mtb CYP metabolism. To this end, this study aimed to biochemically characterize and identify cognate redox partnerships for Mtb CYPs. Interestingly, all combinations of FNRs and ferredoxins were active in the reduction of oxidized cytochrome c, but steady-state kinetic assays revealed FdxD as the most efficient redox partner for FdrA, whereas Fdx coupled preferably with FprA. CYP121A1, CYP124A1, CYP125A1, and CYP142A1 metabolism with the cognate redox partners was reconstituted in vitro showing an unanticipated selectivity in the requirement for electron transfer partnership, which did not necessarily correlate with proximity in the genome. This is the first description of microbial P450 metabolism in which multiple ferredoxins are functionally linked to multiple CYPs.

KW - Cytochrome P450

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