Liver DNA methylation analysis in adult female C57BL/6JxFVB mice following perinatal exposure to bisphenol A.

C.J. van Esterik; A.P. Vitens; H.M. Hodemaeker; J.H. Kamstra; J. Legler; L. Pennings; W.T. Steegenga; C. Lute; J. Jelinek; J.P.J. Issa; M.E.T. Dollé; L.T. van der Ven

doi:10.1016/j.toxlet.2014.10.021

Liver DNA methylation analysis in adult female C57BL/6JxFVB mice following perinatal exposure to bisphenol A.

C.J. van Esterik, A.P. Vitens, H.M. Hodemaeker, J.H. Kamstra, J. Legler, L. Pennings, W.T. Steegenga, C. Lute, J. Jelinek, J.P.J. Issa, M.E.T. Dollé, L.T. van der Ven

Chemistry and Biology

Research output: Contribution to Journal › Article › Academic › peer-review

Abstract

Bisphenol A (BPA) is a compound released from plastics and other consumer products used in everyday life. BPA exposure early in fetal development is proposed to contribute to programming of chronic diseases like obesity and diabetes, by affecting DNA methylation levels. Previously, we showed that in utero and lactational exposure of C57BL/6JxFVB hybrid mice via maternal feed using a dose range of 0-3000 μg/kg body weight/day resulted in a sex-dependent altered metabolic phenotype in offspring at 23 weeks of age. The most univocal effects were observed in females, with reduced body weights and related metabolic effects associated with perinatal BPA exposure. To identify whether the effects of BPA in females are associated with changes in DNA methylation, this was analyzed in liver, which is important in energy homeostasis. Measurement of global DNA methylation did not show any changes. Genome-wide DNA methylation analysis at specific CpG sites in control and 3000 μg/kg body weight/day females with the digital restriction enzyme analysis of methylation (DREAM) assay revealed potential differences, that could, however, not be confirmed by bisulfite pyrosequencing. Overall, we demonstrated that the observed altered metabolic phenotype in female offspring after maternal exposure to BPA was not detectably associated with liver DNA methylation changes. Still, other tissues may be more informative.

Original language	English
Pages (from-to)	293-300
Journal	Toxicology Letters
Volume	232
Issue number	1
Early online date	17 Oct 2014
DOIs	https://doi.org/10.1016/j.toxlet.2014.10.021
Publication status	Published - 2015

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van Esterik, C. J., Vitens, A. P., Hodemaeker, H. M., Kamstra, J. H., Legler, J., Pennings, L., Steegenga, W. T., Lute, C., Jelinek, J., Issa, J. P. J., Dollé, M. E. T., & van der Ven, L. T. (2015). Liver DNA methylation analysis in adult female C57BL/6JxFVB mice following perinatal exposure to bisphenol A. Toxicology Letters, 232(1), 293-300. https://doi.org/10.1016/j.toxlet.2014.10.021

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title = "Liver DNA methylation analysis in adult female C57BL/6JxFVB mice following perinatal exposure to bisphenol A.",

abstract = "Bisphenol A (BPA) is a compound released from plastics and other consumer products used in everyday life. BPA exposure early in fetal development is proposed to contribute to programming of chronic diseases like obesity and diabetes, by affecting DNA methylation levels. Previously, we showed that in utero and lactational exposure of C57BL/6JxFVB hybrid mice via maternal feed using a dose range of 0-3000 μg/kg body weight/day resulted in a sex-dependent altered metabolic phenotype in offspring at 23 weeks of age. The most univocal effects were observed in females, with reduced body weights and related metabolic effects associated with perinatal BPA exposure. To identify whether the effects of BPA in females are associated with changes in DNA methylation, this was analyzed in liver, which is important in energy homeostasis. Measurement of global DNA methylation did not show any changes. Genome-wide DNA methylation analysis at specific CpG sites in control and 3000 μg/kg body weight/day females with the digital restriction enzyme analysis of methylation (DREAM) assay revealed potential differences, that could, however, not be confirmed by bisulfite pyrosequencing. Overall, we demonstrated that the observed altered metabolic phenotype in female offspring after maternal exposure to BPA was not detectably associated with liver DNA methylation changes. Still, other tissues may be more informative.",

author = "{van Esterik}, C.J. and A.P. Vitens and H.M. Hodemaeker and J.H. Kamstra and J. Legler and L. Pennings and W.T. Steegenga and C. Lute and J. Jelinek and J.P.J. Issa and M.E.T. Doll{\'e} and {van der Ven}, L.T.",

year = "2015",

doi = "10.1016/j.toxlet.2014.10.021",

language = "English",

volume = "232",

pages = "293--300",

journal = "Toxicology Letters",

issn = "0378-4274",

publisher = "Elsevier BV",

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van Esterik, CJ, Vitens, AP, Hodemaeker, HM, Kamstra, JH, Legler, J, Pennings, L, Steegenga, WT, Lute, C, Jelinek, J, Issa, JPJ, Dollé, MET & van der Ven, LT 2015, 'Liver DNA methylation analysis in adult female C57BL/6JxFVB mice following perinatal exposure to bisphenol A.', Toxicology Letters, vol. 232, no. 1, pp. 293-300. https://doi.org/10.1016/j.toxlet.2014.10.021

TY - JOUR

T1 - Liver DNA methylation analysis in adult female C57BL/6JxFVB mice following perinatal exposure to bisphenol A.

AU - van Esterik, C.J.

AU - Vitens, A.P.

AU - Hodemaeker, H.M.

AU - Kamstra, J.H.

AU - Legler, J.

AU - Pennings, L.

AU - Steegenga, W.T.

AU - Lute, C.

AU - Jelinek, J.

AU - Issa, J.P.J.

AU - Dollé, M.E.T.

AU - van der Ven, L.T.

PY - 2015

Y1 - 2015

N2 - Bisphenol A (BPA) is a compound released from plastics and other consumer products used in everyday life. BPA exposure early in fetal development is proposed to contribute to programming of chronic diseases like obesity and diabetes, by affecting DNA methylation levels. Previously, we showed that in utero and lactational exposure of C57BL/6JxFVB hybrid mice via maternal feed using a dose range of 0-3000 μg/kg body weight/day resulted in a sex-dependent altered metabolic phenotype in offspring at 23 weeks of age. The most univocal effects were observed in females, with reduced body weights and related metabolic effects associated with perinatal BPA exposure. To identify whether the effects of BPA in females are associated with changes in DNA methylation, this was analyzed in liver, which is important in energy homeostasis. Measurement of global DNA methylation did not show any changes. Genome-wide DNA methylation analysis at specific CpG sites in control and 3000 μg/kg body weight/day females with the digital restriction enzyme analysis of methylation (DREAM) assay revealed potential differences, that could, however, not be confirmed by bisulfite pyrosequencing. Overall, we demonstrated that the observed altered metabolic phenotype in female offspring after maternal exposure to BPA was not detectably associated with liver DNA methylation changes. Still, other tissues may be more informative.

AB - Bisphenol A (BPA) is a compound released from plastics and other consumer products used in everyday life. BPA exposure early in fetal development is proposed to contribute to programming of chronic diseases like obesity and diabetes, by affecting DNA methylation levels. Previously, we showed that in utero and lactational exposure of C57BL/6JxFVB hybrid mice via maternal feed using a dose range of 0-3000 μg/kg body weight/day resulted in a sex-dependent altered metabolic phenotype in offspring at 23 weeks of age. The most univocal effects were observed in females, with reduced body weights and related metabolic effects associated with perinatal BPA exposure. To identify whether the effects of BPA in females are associated with changes in DNA methylation, this was analyzed in liver, which is important in energy homeostasis. Measurement of global DNA methylation did not show any changes. Genome-wide DNA methylation analysis at specific CpG sites in control and 3000 μg/kg body weight/day females with the digital restriction enzyme analysis of methylation (DREAM) assay revealed potential differences, that could, however, not be confirmed by bisulfite pyrosequencing. Overall, we demonstrated that the observed altered metabolic phenotype in female offspring after maternal exposure to BPA was not detectably associated with liver DNA methylation changes. Still, other tissues may be more informative.

U2 - 10.1016/j.toxlet.2014.10.021

DO - 10.1016/j.toxlet.2014.10.021

M3 - Article

SN - 0378-4274

VL - 232

SP - 293

EP - 300

JO - Toxicology Letters

JF - Toxicology Letters

IS - 1

ER -

Liver DNA methylation analysis in adult female C57BL/6JxFVB mice following perinatal exposure to bisphenol A.

Abstract

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