Load-dependent release limits the processive stepping of the tetrameric Eg5 motor

M. Korneev, S. Lakamper, C. Schmidt

Research output: Contribution to JournalArticleAcademicpeer-review

Abstract

Tetrameric motor proteins of the Kinesin-5 family are essential for eukaryotic cell division. The microscopic mechanism by which Eg5, the vertebrate Kinesin-5, drives bipolar mitotic spindle formation remains unknown. Here we show in optical trapping experiments that full-length Eg5 moves processively and stepwise along microtubule bundles. Interestingly, the force produced by individual Eg5 motors typically reached only ∼2 pN, one-third of the stall force of Kinesin-1. Eg5 typically detached from microtubules before stalling. This behavior may reflect a regulatory mechanism important for the role of Eg5 in the mitotic spindle. © 2007 EBSA.
Original languageEnglish
Pages (from-to)675-681
JournalEuropean Biophysics Journal
Volume36
Issue number6
DOIs
Publication statusPublished - 2007

Bibliographical note

Load-dependent release limits the processive stepping of the tetrameric Eg5 motor

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