Localization of c-ERB A proteins in rat liver using monoclonal antibodies

E.D.L. Schmidt; H.C. van Beeren; H. Korfage; J.H. Dussault; W.M. Wiersinga; W.H. Lamers

doi:10.1016/0006-291X(89)91776-2

Localization of c-ERB A proteins in rat liver using monoclonal antibodies

E.D.L. Schmidt, H.C. van Beeren, H. Korfage, J.H. Dussault, W.M. Wiersinga, W.H. Lamers

Research output: Contribution to Journal › Article › Academic › peer-review

Abstract

Monoclonal antibodies were raised against the nuclear thyroid hormone receptors encoded by c-ERB A genes and against a purified nuclear receptor fraction. These antibodies recognize the c-ERB A protein in nuclear extracts from rat liver and are able to compete with thyroid hormone in Scatchard analyses. In sections of rat liver they react with all the hepatocyte nuclei as well as with the cells of the hepatic bile ducts. Comparison with another putative T3 receptor antibody, described previously, showed that distinct 57 kD proteins with a different cellular distribution were recognized. © 1989.

Original language	English
Pages (from-to)	1053-1059
Journal	Biochemical and Biophysical Research Communications
Volume	164
Issue number	3
DOIs	https://doi.org/10.1016/0006-291X(89)91776-2
Publication status	Published - 15 Nov 1989
Externally published	Yes

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10.1016/0006-291X(89)91776-2

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abstract = "Monoclonal antibodies were raised against the nuclear thyroid hormone receptors encoded by c-ERB A genes and against a purified nuclear receptor fraction. These antibodies recognize the c-ERB A protein in nuclear extracts from rat liver and are able to compete with thyroid hormone in Scatchard analyses. In sections of rat liver they react with all the hepatocyte nuclei as well as with the cells of the hepatic bile ducts. Comparison with another putative T3 receptor antibody, described previously, showed that distinct 57 kD proteins with a different cellular distribution were recognized. {\textcopyright} 1989.",

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AU - Wiersinga, W.M.

AU - Lamers, W.H.

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AB - Monoclonal antibodies were raised against the nuclear thyroid hormone receptors encoded by c-ERB A genes and against a purified nuclear receptor fraction. These antibodies recognize the c-ERB A protein in nuclear extracts from rat liver and are able to compete with thyroid hormone in Scatchard analyses. In sections of rat liver they react with all the hepatocyte nuclei as well as with the cells of the hepatic bile ducts. Comparison with another putative T3 receptor antibody, described previously, showed that distinct 57 kD proteins with a different cellular distribution were recognized. © 1989.

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JO - Biochemical and Biophysical Research Communications

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ER -

Localization of c-ERB A proteins in rat liver using monoclonal antibodies

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