Localization of Fatty Acyl and Double Bond Positions in Phosphatidylcholines Using a Dual Stage CID Fragmentation Coupled with Ion Mobility Mass Spectrometry

J. Castro-Perez, T.P. Roddy, N.M.M. Nibbering, V. Shah, D.G. McLaren, S. Previs, A.B. Attygalle, K. Herath, Z. Chen, S.P. Wang, L. Mitnaul, B.K. Hubbard, R.J. Vreeken, D.G. Johns, Th. Hankemeier

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Abstract

A high content molecular fragmentation for the analysis of phosphatidylcholines (PC) was achieved utilizing a two-stage [trap (first generation fragmentation) and transfer (second generation fragmentation)] collision-induced dissociation (CID) in combination with travelling-wave ion mobility spectrometry (TWIMS). The novel aspects of this work reside in the fact that a TWIMS arrangement was used to obtain a high level structural information including location of fatty acyl substituents and double bonds for PCs in plasma, and the presence of alkali metal adduct ions such as [M + Li]
Original languageEnglish
Pages (from-to)1552-1567
JournalJournal of the American Society for Mass Spectrometry
Volume22
Issue number9
DOIs
Publication statusPublished - 2011

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