Luciferase detection during stationary phase in Lactococcus lactis

Herwig Bachmann, Filipe Branco dos Santos, Michiel Kleerebezem, Johan E T Van Hylckama Vlieg

Research output: Contribution to JournalArticleAcademicpeer-review


The luminescence signal of luxAB-encoded bacterial luciferase strongly depends on the metabolic state of the host cell, which restricts the use of this reporter system to metabolically active bacteria. Here we show that in stationary-phase cells of Lactococcus lactis, detection of luciferase is significantly improved by the addition of riboflavin or flavin mononucleotide to whole-cell assay systems.
Original languageEnglish
Pages (from-to)4704-4706
Number of pages3
JournalApplied and Environmental Microbiology
Publication statusPublished - Jul 2007


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