Lymphoblast-derived hiPS cell lines generated from four individuals of a family of genetically unrelated parents and their female monozygotic twins

Marga J. Bouma, Christian Freund, Adriaan P. IJzerman, Dorret I. Boomsma, Christine L. Mummery, Karine Raymond*

*Corresponding author for this work

Research output: Contribution to JournalArticleAcademicpeer-review

Abstract

Lymphoblast cells from four individuals of a family of two genetically unrelated parents and their monozygotic twins were used to generate integration-free human induced pluripotent stem cells (hiPSCs). Reprogramming factors were delivered by co-electroporation of three episomal-based plasmids expressing OCT3/4, SOX2, KLF4, L-MYC and LIN28. The hiPSCs showed a normal karyotype, expressed pluripotency-associated markers, displayed the capacity for in vitro differentiation into the three germ layers and were Epstein Barr virus-free. These hiPSC lines offer the possibility to compare genetically unrelated and genetically identical tissues from different individuals and to study genotype-specific effects, which are particularly relevant for toxicology testing.

Original languageEnglish
Article number101654
Pages (from-to)1-5
Number of pages5
JournalStem Cell Research
Volume41
Early online date8 Nov 2019
DOIs
Publication statusPublished - Dec 2019

Bibliographical note

Copyright © 2019 The Authors. Published by Elsevier B.V. All rights reserved.

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