Lymphoblast-derived hiPS cell lines generated from four individuals of a family of genetically unrelated parents and their female monozygotic twins

Marga J. Bouma; Christian Freund; Adriaan P. IJzerman; Dorret I. Boomsma; Christine L. Mummery; Karine Raymond

doi:10.1016/j.scr.2019.101654

Lymphoblast-derived hiPS cell lines generated from four individuals of a family of genetically unrelated parents and their female monozygotic twins

Marga J. Bouma, Christian Freund, Adriaan P. IJzerman, Dorret I. Boomsma, Christine L. Mummery, Karine Raymond^*

^*Corresponding author for this work

Research output: Contribution to Journal › Article › Academic › peer-review

Abstract

Lymphoblast cells from four individuals of a family of two genetically unrelated parents and their monozygotic twins were used to generate integration-free human induced pluripotent stem cells (hiPSCs). Reprogramming factors were delivered by co-electroporation of three episomal-based plasmids expressing OCT3/4, SOX2, KLF4, L-MYC and LIN28. The hiPSCs showed a normal karyotype, expressed pluripotency-associated markers, displayed the capacity for in vitro differentiation into the three germ layers and were Epstein Barr virus-free. These hiPSC lines offer the possibility to compare genetically unrelated and genetically identical tissues from different individuals and to study genotype-specific effects, which are particularly relevant for toxicology testing.

Original language	English
Article number	101654
Pages (from-to)	1-5
Number of pages	5
Journal	Stem Cell Research
Volume	41
Early online date	8 Nov 2019
DOIs	https://doi.org/10.1016/j.scr.2019.101654
Publication status	Published - Dec 2019

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title = "Lymphoblast-derived hiPS cell lines generated from four individuals of a family of genetically unrelated parents and their female monozygotic twins",

abstract = "Lymphoblast cells from four individuals of a family of two genetically unrelated parents and their monozygotic twins were used to generate integration-free human induced pluripotent stem cells (hiPSCs). Reprogramming factors were delivered by co-electroporation of three episomal-based plasmids expressing OCT3/4, SOX2, KLF4, L-MYC and LIN28. The hiPSCs showed a normal karyotype, expressed pluripotency-associated markers, displayed the capacity for in vitro differentiation into the three germ layers and were Epstein Barr virus-free. These hiPSC lines offer the possibility to compare genetically unrelated and genetically identical tissues from different individuals and to study genotype-specific effects, which are particularly relevant for toxicology testing.",

author = "Bouma, {Marga J.} and Christian Freund and IJzerman, {Adriaan P.} and Boomsma, {Dorret I.} and Mummery, {Christine L.} and Karine Raymond",

year = "2019",

month = dec,

doi = "10.1016/j.scr.2019.101654",

language = "English",

volume = "41",

pages = "1--5",

journal = "Stem Cell Research",

issn = "1873-5061",

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Lymphoblast-derived hiPS cell lines generated from four individuals of a family of genetically unrelated parents and their female monozygotic twins. / Bouma, Marga J.; Freund, Christian; IJzerman, Adriaan P.; Boomsma, Dorret I.; Mummery, Christine L.; Raymond, Karine.

In: Stem Cell Research, Vol. 41, 101654, 12.2019, p. 1-5.

Research output: Contribution to Journal › Article › Academic › peer-review

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T1 - Lymphoblast-derived hiPS cell lines generated from four individuals of a family of genetically unrelated parents and their female monozygotic twins

AU - Bouma, Marga J.

AU - Freund, Christian

AU - IJzerman, Adriaan P.

AU - Boomsma, Dorret I.

AU - Mummery, Christine L.

AU - Raymond, Karine

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AB - Lymphoblast cells from four individuals of a family of two genetically unrelated parents and their monozygotic twins were used to generate integration-free human induced pluripotent stem cells (hiPSCs). Reprogramming factors were delivered by co-electroporation of three episomal-based plasmids expressing OCT3/4, SOX2, KLF4, L-MYC and LIN28. The hiPSCs showed a normal karyotype, expressed pluripotency-associated markers, displayed the capacity for in vitro differentiation into the three germ layers and were Epstein Barr virus-free. These hiPSC lines offer the possibility to compare genetically unrelated and genetically identical tissues from different individuals and to study genotype-specific effects, which are particularly relevant for toxicology testing.

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Lymphoblast-derived hiPS cell lines generated from four individuals of a family of genetically unrelated parents and their female monozygotic twins

Abstract

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