Abstract
Lymphoblast cells from four individuals of a family of two genetically unrelated parents and their monozygotic twins were used to generate integration-free human induced pluripotent stem cells (hiPSCs). Reprogramming factors were delivered by co-electroporation of three episomal-based plasmids expressing OCT3/4, SOX2, KLF4, L-MYC and LIN28. The hiPSCs showed a normal karyotype, expressed pluripotency-associated markers, displayed the capacity for in vitro differentiation into the three germ layers and were Epstein Barr virus-free. These hiPSC lines offer the possibility to compare genetically unrelated and genetically identical tissues from different individuals and to study genotype-specific effects, which are particularly relevant for toxicology testing.
Original language | English |
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Article number | 101654 |
Pages (from-to) | 1-5 |
Number of pages | 5 |
Journal | Stem Cell Research |
Volume | 41 |
Early online date | 8 Nov 2019 |
DOIs | |
Publication status | Published - Dec 2019 |
Bibliographical note
Copyright © 2019 The Authors. Published by Elsevier B.V. All rights reserved.Funding
We would like to thank the Department of Human Genetics, LUMC, for the STR DNA analysis and the Laboratorium voor Diagnostische Genoomanalyse (LGDA), LUMC, for the karyotyping analysis. This work was supported by the Netherlands Organ-on-Chip Initiative, an NOW Gravitation project funded by the Ministry of Education , Culture and Science of the government of the Netherlands ( 024.003.001 ).
Funders | Funder number |
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Department of Human Genetics | |
LGDA | |
Laboratorium voor Diagnostische Genoomanalyse | |
Ministry of Education , Culture and Science | 024.003.001 |
Netherlands Organ-on-Chip Initiative | |
Ministerie van Onderwijs, Cultuur en Wetenschap | |
Leids Universitair Medisch Centrum |
Cohort Studies
- Netherlands Twin Register (NTR)