Lymphoblast-derived hiPS cell lines generated from four individuals of a family of genetically unrelated parents and their female monozygotic twins

Marga J. Bouma, Christian Freund, Adriaan P. IJzerman, Dorret I. Boomsma, Christine L. Mummery, Karine Raymond*

*Corresponding author for this work

Research output: Contribution to JournalArticleAcademicpeer-review

Abstract

Lymphoblast cells from four individuals of a family of two genetically unrelated parents and their monozygotic twins were used to generate integration-free human induced pluripotent stem cells (hiPSCs). Reprogramming factors were delivered by co-electroporation of three episomal-based plasmids expressing OCT3/4, SOX2, KLF4, L-MYC and LIN28. The hiPSCs showed a normal karyotype, expressed pluripotency-associated markers, displayed the capacity for in vitro differentiation into the three germ layers and were Epstein Barr virus-free. These hiPSC lines offer the possibility to compare genetically unrelated and genetically identical tissues from different individuals and to study genotype-specific effects, which are particularly relevant for toxicology testing.

Original languageEnglish
Article number101654
Pages (from-to)1-5
Number of pages5
JournalStem Cell Research
Volume41
Early online date8 Nov 2019
DOIs
Publication statusPublished - Dec 2019

Bibliographical note

Copyright © 2019 The Authors. Published by Elsevier B.V. All rights reserved.

Funding

We would like to thank the Department of Human Genetics, LUMC, for the STR DNA analysis and the Laboratorium voor Diagnostische Genoomanalyse (LGDA), LUMC, for the karyotyping analysis. This work was supported by the Netherlands Organ-on-Chip Initiative, an NOW Gravitation project funded by the Ministry of Education , Culture and Science of the government of the Netherlands ( 024.003.001 ).

FundersFunder number
Department of Human Genetics
LGDA
Laboratorium voor Diagnostische Genoomanalyse
Ministry of Education , Culture and Science024.003.001
Netherlands Organ-on-Chip Initiative
Ministerie van Onderwijs, Cultuur en Wetenschap
Leids Universitair Medisch Centrum

    Cohort Studies

    • Netherlands Twin Register (NTR)

    Fingerprint

    Dive into the research topics of 'Lymphoblast-derived hiPS cell lines generated from four individuals of a family of genetically unrelated parents and their female monozygotic twins'. Together they form a unique fingerprint.

    Cite this