Mapping localization of 21 endogenous proteins in the Golgi apparatus of rodent neurons

Danique M. van Bommel; Ruud F. Toonen; Matthijs Verhage

doi:10.1038/s41598-023-29998-8

Mapping localization of 21 endogenous proteins in the Golgi apparatus of rodent neurons

Danique M. van Bommel, Ruud F. Toonen, Matthijs Verhage^*

^*Corresponding author for this work

Research output: Contribution to Journal › Article › Academic › peer-review

Abstract

The Golgi apparatus is the major sorting hub in the secretory pathway and particularly important for protein sorting in neurons. Knowledge about protein localization in Golgi compartments is largely based on work in cell lines. Here, we systematically compared protein localization of 21 endogenous proteins in the Golgi apparatus of mouse neurons using confocal microscopy and line scan analysis. We localized these proteins by measuring the distance relative to the canonical TGN marker TGN38. Based on this, proteins fell into three groups: upstream of, overlapping with or downstream of TGN38. Seven proteins showed complete overlap with TGN38, while proteins downstream of TGN38 were located at varying distances from TGN38. Proteins upstream of TGN38 were localized in between TGN38 and the cis-/medial Golgi markers Giantin and GM130. This localization was consistent with protein function. Our data provide an overview of the relative localization of endogenous proteins in the Golgi of primary mouse neurons.

Original language	English
Article number	2871
Pages (from-to)	1-9
Number of pages	9
Journal	Scientific Reports
Volume	13
DOIs	https://doi.org/10.1038/s41598-023-29998-8
Publication status	Published - 18 Feb 2023

Bibliographical note

Funding Information:
We thank Lisa Laan, Ingrid Saarloos and Desiree Schut for producing glia and for primary culture assistance and Joke Wortel for animal breeding. We acknowledge the Microscopy and Cytometry Core Facility at the Amsterdam UMC – Location VUmc for providing assistance in microscopy. This work is supported by a European Research Council Advanced Grant (322966) of the European Union (to M.V.).

Publisher Copyright:
© 2023, The Author(s).

Funding

We thank Lisa Laan, Ingrid Saarloos and Desiree Schut for producing glia and for primary culture assistance and Joke Wortel for animal breeding. We acknowledge the Microscopy and Cytometry Core Facility at the Amsterdam UMC – Location VUmc for providing assistance in microscopy. This work is supported by a European Research Council Advanced Grant (322966) of the European Union (to M.V.).

Funders	Funder number
European Research Council
European Commission
Seventh Framework Programme	322966

Access to Document

10.1038/s41598-023-29998-8

Persistent URL (handle)

Persistent link

Cite this

@article{c49c5a55ec3d4b60a2bbc2cb9568b373,

title = "Mapping localization of 21 endogenous proteins in the Golgi apparatus of rodent neurons",

abstract = "The Golgi apparatus is the major sorting hub in the secretory pathway and particularly important for protein sorting in neurons. Knowledge about protein localization in Golgi compartments is largely based on work in cell lines. Here, we systematically compared protein localization of 21 endogenous proteins in the Golgi apparatus of mouse neurons using confocal microscopy and line scan analysis. We localized these proteins by measuring the distance relative to the canonical TGN marker TGN38. Based on this, proteins fell into three groups: upstream of, overlapping with or downstream of TGN38. Seven proteins showed complete overlap with TGN38, while proteins downstream of TGN38 were located at varying distances from TGN38. Proteins upstream of TGN38 were localized in between TGN38 and the cis-/medial Golgi markers Giantin and GM130. This localization was consistent with protein function. Our data provide an overview of the relative localization of endogenous proteins in the Golgi of primary mouse neurons.",

author = "{van Bommel}, {Danique M.} and Toonen, {Ruud F.} and Matthijs Verhage",

note = "Funding Information: We thank Lisa Laan, Ingrid Saarloos and Desiree Schut for producing glia and for primary culture assistance and Joke Wortel for animal breeding. We acknowledge the Microscopy and Cytometry Core Facility at the Amsterdam UMC – Location VUmc for providing assistance in microscopy. This work is supported by a European Research Council Advanced Grant (322966) of the European Union (to M.V.). Publisher Copyright: {\textcopyright} 2023, The Author(s).",

year = "2023",

month = feb,

day = "18",

doi = "10.1038/s41598-023-29998-8",

language = "English",

volume = "13",

pages = "1--9",

journal = "Scientific Reports",

issn = "2045-2322",

publisher = "Nature Research",

}

TY - JOUR

T1 - Mapping localization of 21 endogenous proteins in the Golgi apparatus of rodent neurons

AU - van Bommel, Danique M.

AU - Toonen, Ruud F.

AU - Verhage, Matthijs

N1 - Funding Information: We thank Lisa Laan, Ingrid Saarloos and Desiree Schut for producing glia and for primary culture assistance and Joke Wortel for animal breeding. We acknowledge the Microscopy and Cytometry Core Facility at the Amsterdam UMC – Location VUmc for providing assistance in microscopy. This work is supported by a European Research Council Advanced Grant (322966) of the European Union (to M.V.). Publisher Copyright: © 2023, The Author(s).

PY - 2023/2/18

Y1 - 2023/2/18

N2 - The Golgi apparatus is the major sorting hub in the secretory pathway and particularly important for protein sorting in neurons. Knowledge about protein localization in Golgi compartments is largely based on work in cell lines. Here, we systematically compared protein localization of 21 endogenous proteins in the Golgi apparatus of mouse neurons using confocal microscopy and line scan analysis. We localized these proteins by measuring the distance relative to the canonical TGN marker TGN38. Based on this, proteins fell into three groups: upstream of, overlapping with or downstream of TGN38. Seven proteins showed complete overlap with TGN38, while proteins downstream of TGN38 were located at varying distances from TGN38. Proteins upstream of TGN38 were localized in between TGN38 and the cis-/medial Golgi markers Giantin and GM130. This localization was consistent with protein function. Our data provide an overview of the relative localization of endogenous proteins in the Golgi of primary mouse neurons.

AB - The Golgi apparatus is the major sorting hub in the secretory pathway and particularly important for protein sorting in neurons. Knowledge about protein localization in Golgi compartments is largely based on work in cell lines. Here, we systematically compared protein localization of 21 endogenous proteins in the Golgi apparatus of mouse neurons using confocal microscopy and line scan analysis. We localized these proteins by measuring the distance relative to the canonical TGN marker TGN38. Based on this, proteins fell into three groups: upstream of, overlapping with or downstream of TGN38. Seven proteins showed complete overlap with TGN38, while proteins downstream of TGN38 were located at varying distances from TGN38. Proteins upstream of TGN38 were localized in between TGN38 and the cis-/medial Golgi markers Giantin and GM130. This localization was consistent with protein function. Our data provide an overview of the relative localization of endogenous proteins in the Golgi of primary mouse neurons.

UR - http://www.scopus.com/inward/record.url?scp=85148380196&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85148380196&partnerID=8YFLogxK

U2 - 10.1038/s41598-023-29998-8

DO - 10.1038/s41598-023-29998-8

M3 - Article

C2 - 36806293

AN - SCOPUS:85148380196

SN - 2045-2322

VL - 13

SP - 1

EP - 9

JO - Scientific Reports

JF - Scientific Reports

M1 - 2871

ER -

Mapping localization of 21 endogenous proteins in the Golgi apparatus of rodent neurons

Abstract

Bibliographical note

Funding

Access to Document

Persistent URL (handle)

Other files and links

Fingerprint

Cite this