Measuring intracellular viscosity in conditions of hypergravity

E.M. Woodcock; P. Girvan; J. Eckert; I. Lopez-Duarte; M. Kubánková; J.J.W.A. van Loon; N.J. Brooks; M.K. Kuimova

doi:10.1016/j.bpj.2019.03.038

Measuring intracellular viscosity in conditions of hypergravity

E.M. Woodcock, P. Girvan, J. Eckert, I. Lopez-Duarte, M. Kubánková, J.J.W.A. van Loon, N.J. Brooks, M.K. Kuimova

Maxillofacial Surgery (VUmc)

Research output: Contribution to Journal › Article › Academic › peer-review

Abstract

Gravity-sensitive cellular responses are regularly observed in both specialized and nonspecialized cells. One potential mechanism for this sensitivity is a changing viscosity of the intracellular organelles. Here, we report a novel, to our knowledge, viscosity-sensitive molecular rotor based on mesosubstituted boron-dipyrrin used to investigate the response of viscosity of cellular membranes to hypergravity conditions created at the large diameter centrifuge at the European Space Agency Technology Centre. Mouse osteoblastic (MC3T3-E1) and endothelial (human umbilical vein endothelial cell) cell lines were tested, and an increase in viscosity was found with increasing hypergravity loading. This response is thought to be primarily biologically driven, with the potential for a small, instantaneous physical mechanism also contributing to the observed effect. This work provides the first, to our knowledge, quantitative data for cellular viscosity changes under hypergravity, up to 15 × g.

Original language	English
Pages (from-to)	1984-1993
Number of pages	10
Journal	Biophysical Journal
Volume	116
Issue number	10
DOIs	https://doi.org/10.1016/j.bpj.2019.03.038
Publication status	Published - 21 May 2019

Funding

The authors thank Alan Dowson (European Space Agency Technology Centre, Noordwijk, the Netherlands) for technical support. J.E. thanks Jens-Uwe Sommer (Leibniz Institute of Polymer Research Dresden). The authors acknowledge the Spin Your Thesis! 2017 campaign, organized by European Space Agency Education, for supporting this research and European Space Agency grant/contract 4000107455/12/NL/PA awarded to J.J.W.A.v.L. M.K.K. is grateful to the Engineering and Physical Sciences Research Council for the Career Acceleration Fellowship (EP/I003983/1). N.J.B. is grateful to the Engineering and Physical Sciences Research Council for the support of program grant EP/J017566/1. The authors acknowledge the Spin Your Thesis! 2017 campaign, organized by European Space Agency Education , for supporting this research and European Space Agency grant/contract 4000107455/12/NL/PA awarded to J.J.W.A.v.L. M.K.K. is grateful to the Engineering and Physical Sciences Research Council for the Career Acceleration Fellowship ( EP/I003983/1 ). N.J.B. is grateful to the Engineering and Physical Sciences Research Council for the support of program grant EP/J017566/1 .

Funders	Funder number
Alan Dowson
European Space Agency Education
European Space Agency Technology Centre
Engineering and Physical Sciences Research Council	EP/J017566/1, EP/I003983/1
European Space Agency	4000107455/12/NL/PA
Leibniz-Institut fÃ¼r Polymerforschung Dresden

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title = "Measuring intracellular viscosity in conditions of hypergravity",

abstract = "Gravity-sensitive cellular responses are regularly observed in both specialized and nonspecialized cells. One potential mechanism for this sensitivity is a changing viscosity of the intracellular organelles. Here, we report a novel, to our knowledge, viscosity-sensitive molecular rotor based on mesosubstituted boron-dipyrrin used to investigate the response of viscosity of cellular membranes to hypergravity conditions created at the large diameter centrifuge at the European Space Agency Technology Centre. Mouse osteoblastic (MC3T3-E1) and endothelial (human umbilical vein endothelial cell) cell lines were tested, and an increase in viscosity was found with increasing hypergravity loading. This response is thought to be primarily biologically driven, with the potential for a small, instantaneous physical mechanism also contributing to the observed effect. This work provides the first, to our knowledge, quantitative data for cellular viscosity changes under hypergravity, up to 15 × g.",

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AU - Woodcock, E.M.

AU - Girvan, P.

AU - Eckert, J.

AU - Lopez-Duarte, I.

AU - Kubánková, M.

AU - van Loon, J.J.W.A.

AU - Brooks, N.J.

AU - Kuimova, M.K.

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AB - Gravity-sensitive cellular responses are regularly observed in both specialized and nonspecialized cells. One potential mechanism for this sensitivity is a changing viscosity of the intracellular organelles. Here, we report a novel, to our knowledge, viscosity-sensitive molecular rotor based on mesosubstituted boron-dipyrrin used to investigate the response of viscosity of cellular membranes to hypergravity conditions created at the large diameter centrifuge at the European Space Agency Technology Centre. Mouse osteoblastic (MC3T3-E1) and endothelial (human umbilical vein endothelial cell) cell lines were tested, and an increase in viscosity was found with increasing hypergravity loading. This response is thought to be primarily biologically driven, with the potential for a small, instantaneous physical mechanism also contributing to the observed effect. This work provides the first, to our knowledge, quantitative data for cellular viscosity changes under hypergravity, up to 15 × g.

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Measuring intracellular viscosity in conditions of hypergravity

Abstract

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